Morphofunctional dynamics of metamorphosis in eel leptocephali and its application for aquaculture

• Project/Area Number: 25450270
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKI Mari 東京大学, 農学生命科学研究科, 助教 (00568800)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 回遊魚 / ウナギ / レプトセファルス / 変態 / 骨格形成 / 形態異常 / 浸透圧調節

Outline of Final Research Achievements

Morphofunctional dynamics of metamorphosis and occurrence of deformities in reared eel leptocephali were studied to contribute to establishing artificial production techniques of glass eels for eel aquaculture. It was revealed that 1) eight types of vertebral deformity (compression, luxation, fusion, brachyspina, modification, lordosis, kyphosis and scoliosis) that were seldom found in wild glass eels occured. 2) Ionocytes distributing all over the body surface of leptocephali showed immunoreaction of ion transporters such as NKA, NKCC1 and CFTR, and these transporters appeared to play an important role for keeping the ion balance of the leptocephalus body. 3) Leptocephali reared at the higher water velocities in the circulating rearing tank showed smaller body sizes and higher occurrence of notochord curvature suggesting importance of current condition in the tank.

Research Products

• [Journal Article] Geographic analyses of stable isotopic and fatty acid composition of three families of anguilliform leptocephali in the western South Pacific. (2016)
  • Author(s): Lienart Camilla, Eric Feunteun, Michael J. Miller, Jun Aoyama, Jean-Michel Mortillaro, Cedric Hubas, Mari Kuroki, Shun Watanabe, Christine Dupuy, Alexandre Carpentier, Tsuguo Otake, Katsumi Tsukamoto and Tarik Meziane
  • Journal Title: Marine Ecology Progress Series Volume: 544 Pages: 225-241
  • DOI: 10.3354/meps11575
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphofunctional features of ionocytes in Japanese eel Anguilla japonica leptocephali acclimated to half-diluted and full-strength seawater (2016)
  • Author(s): Kuroki, M., Seo M.Y., Okamura, A., Watanabe, S., Tsukamoto, K., Kaneko, T.
  • Journal Title: Ichthyological Research Volume: 63 Issue: 4 Pages: 487-495
  • DOI: 10.1007/s10228-016-0520-0
  • NAID: 40020979343
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Occurrence of larval and adult types of ion-secreting ionocytes in Japanese eel Anguilla japonica (2015)
  • Author(s): Seo Miyoung, Mari Kuroki, Akihiro Okamura, Katsumi Tsukamoto, Soichi Watanabe and Toyoji Kaneko
  • Journal Title: Ichthyological Research Volume: - Issue: 4 Pages: 000-000
  • DOI: 10.1007/s10228-015-0463-x
  • NAID: 40020644665
  • Peer Reviewed
• [Journal Article] Stable isotopic composition of anguilliform leptocephali and other food web components from west of the Mascarene Plateau (2015)
  • Author(s): Feunteun E, Miller MJ, Carpentier A, Aoyama J, Dupuy C, Kuroki M, Pagano M, Reveillac E, Sellos D, Watanabe S, Tsukamoto K, Otake T
  • Journal Title: Progress in Oceanography Volume: 137 Pages: 235-246
  • DOI: 10.1016/j.pocean.2015.05.024
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Biodiversity and distribution of leptocephali west of the Mascarene Plateau in the southwestern Indian Ocean (2015)
  • Author(s): Miller MJ, Feunteun E, Aoyama J, Watanabe S, Kuroki M, Lecomte-Finiger R, Minegishi Y, Robinet T, Reveillac E, Gagnaire PA, Berrebi P, Tsukamoto K, Otake T
  • Journal Title: Progress in Oceanography Volume: 137 Pages: 84-102
  • DOI: 10.1016/j.pocean.2015.05.026
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diversity of early life history traits in freshwater eels and the evolution of their oceanic migrations (2014)
  • Author(s): Kuroki, M., Miller, M.J., Tsukamoto, K.
  • Journal Title: Canadian Journal of Zoology Volume: 92(9) Issue: 9 Pages: 749-770
  • DOI: 10.1139/cjz-2013-0303
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Changes in the role of the thyroid axis during metamorphosis of the Japanese eel, Anguilla japonica (2014)
  • Author(s): Sudo, R., Okamura, A., Kuroki, M., Tsukamoto, K.
  • Journal Title: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Volume: 321(7) Issue: 7 Pages: 356-364
  • DOI: 10.1002/jez.1861
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Early larval growth and hatching time of Atlantic eels in the Sargasso Sea (2015)
  • Author(s): Kuroki, M., Marohn, L., Miller M.J., Wysujack, K., Tsukamoto, K., Hanel, R.
  • Organizer: 39th Annual Larval Fish Conference
  • Place of Presentation: ウィーン（オーストリア）
  • Year and Date: 2015-07-16
• [Presentation] Public education for eel conservation (2015)
  • Author(s): Kuroki, M.
  • Organizer: Eel Planet International Symposium
  • Place of Presentation: 日本大学会館（東京都千代田区）
  • Year and Date: 2015-06-28
  • Invited
• [Presentation] ニホンウナギ仔魚の飼育水槽の水流が脊椎骨異常の発症に及ぼす影響 (2015)
  • Author(s): 竹内綾, 黒木真理, 岡村明浩，塚本勝巳
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都品川区）
  • Year and Date: 2015-03-30
• [Presentation] ニホンウナギとAnguilla mossambicaの雑種は仔魚期間が短い (2015)
  • Author(s): 須藤竜介, 山田祥朗, 黒木真理, 塚本勝巳, 坂本崇
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都品川区）
  • Year and Date: 2015-03-30
• [Presentation] Status and trend of the Japanese eel resources－ecological research for eel conservation (2014)
  • Author(s): Kuroki, M.
  • Organizer: International symposium on conservation, management and trade of Anguilla bicolor eel in Indonesia
  • Place of Presentation: ジャカルタ（インドネシア）
  • Year and Date: 2014-11-25
  • Invited
• [Presentation] 亜熱帯循環系におけるウナギ目を中心とした魚類稚仔魚の分布特性 (2014)
  • Author(s): 恩田拓尭, 竹茂愛吾, 三宅陽一, Michael J. Miller, 黒木真理, 青山潤, 木村伸吾
  • Organizer: 水産海洋学会
  • Place of Presentation: 独立行政法人水産総合研究センター中央水産研究所（神奈川県横浜市）
  • Year and Date: 2014-11-16
• [Presentation] 北赤道海流域における物理・生物学的調査研究 (2) ウナギ目仔魚の分布 (2014)
  • Author(s): 恩田拓尭, 竹茂愛吾, 三宅陽一, Michael J. Miller, 黒木真理, 青山潤, 木村伸吾
  • Organizer: 日本水産学会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡県福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] 北赤道海流域における物理・生物学的調査研究 (2) ニホンウナギ仔魚の分布 (2014)
  • Author(s): 竹茂愛吾, 恩田拓尭, 三宅陽一, Michael J. Miller, 黒木真理, 青山潤, 木村伸吾
  • Organizer: 日本海洋学会
  • Place of Presentation: 長崎大学文教キャンパス（長崎県長崎市）
  • Year and Date: 2014-09-14 – 2014-09-16
• [Presentation] ニホンウナギ仔魚の塩類細胞 (2014)
  • Author(s): 徐美暎, 黒木真理, 岡村明浩, 塚本勝巳, 渡邊壮一, 金子豊二
  • Organizer: うな丼の未来II―人とうなぎの共存をめざして
  • Place of Presentation: 東京大学弥生キャンパス（東京都文京区）
  • Year and Date: 2014-07-27
• [Presentation] ニホンウナギ仔魚の塩類細胞に関する機能形態学的特性 (2014)
  • Author(s): 徐美暎・黒木真理・岡村明浩・塚本勝巳・渡邊壮一・金子豊二
  • Organizer: 日本水産学会
  • Place of Presentation: 北海道大学水産学部（北海道函館市）