Development of new culture techniques to reduce skin fluke infection in fish farms

• Project/Area Number: 25450284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Kinki University
• Principal Investigator: SHIRAKASHI Sho 近畿大学, 水産研究所, 講師 (70565936)
• Co-Investigator(Kenkyū-buntansha): OGAWA Kazuo 目黒寄生虫館, 館長 (20092174)
• Co-Investigator(Renkei-kenkyūsha): TSUDA Yuichi 元近畿大学, 水産研究所, 博士研究員 (50508322)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 魚病 / 寄生虫 / 生態 / 養殖 / 防除 / 単生類

Outline of Final Research Achievements

Skin fluke has been a chronic problem in aquaculture. The present study aims to understand the biology and ecology of Neobenedenia girellae and to develop practical methods to prevent infection in fish farms. Larval fluke are positively phototactic and the infection took place during mid-day near the water surface. Shading of culture cage successfully reduced the infection. An adult fluke laid over 700 eggs per day and the eggs accumulated on the culture cages at the maximum rate of over ten million eggs/day. Large proportions of eggs were attached on the upper part of the cages. Using these newly obtained information, we have proposed effective culture techniques to remove eggs from cage and to reduce infection in fish farms.

Research Products

• [Journal Article] Egg Laying and Hatching Rhythms of the Skin Fluke <i>Neobenedenia girellae</i> (2015)
  • Author(s): 平野千早, 石丸克也,白樫正
  • Journal Title: Fish Pathology Volume: 50 Issue: 1 Pages: 23-28
  • DOI: 10.3147/jsfp.50.23
  • NAID: 130005129563
  • ISSN: 0388-788X, 1881-7335
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Accumulation and distribution of skin fluke Neobenedenia girellae eggs on a culture cage (2015)
  • Author(s): Shirakashi, S., Hirano, C.
  • Journal Title: Aquaculture Volume: 443 Pages: 1-4
  • DOI: 10.1016/j.aquaculture.2015.02.036
  • Open Access / Acknowledgement Compliant
• [Journal Article] Shading of net cage as is an effective control measure against skin fluke Neobenedenia girellae infection in chub mackerel Scomber japonicus (2014)
  • Author(s): Yamamoto, S., Fukushima, A., Ishimaru, K.,Shirakashi, S.,
  • Journal Title: Fisheries Science Volume: 80 Issue: 5 Pages: 1021-1026
  • DOI: 10.1007/s12562-014-0781-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Insemination of the monogenean Neobenedenia girellae (Capsalidae, Benedeniinae) (2014)
  • Author(s): Ogawa, K. Shirakashi S., Ishitani H.
  • Journal Title: Parasitology International Volume: 63 Issue: 2 Pages: 473-478
  • DOI: 10.1016/j.parint.2013.10.009
  • Peer Reviewed
• [Journal Article] Diurnal pattern of skin fluke infection in cultured amberjack, Seriola dumerili, at different water depths (2013)
  • Author(s): Shirakashi S., Hirano C., Ishitani H., Ishimaru K.
  • Journal Title: Aquaculture Volume: 402-403 Pages: 19-23
  • DOI: 10.1016/j.aquaculture.2013.03.014
  • Peer Reviewed
• [Journal Article] Shading Reduces Neobenedenia girellae Infection on Cultured Greater Amberjack Seriola dumeri (2013)
  • Author(s): Shirakashi S., Hirano C., Asmara A, Noor N., Ishimaru K., Miyashita S.
  • Journal Title: Fish Patholog Volume: 48 Pages: 25-28
  • Peer Reviewed
• [Presentation] ハダムシNeobenedenia girellaeの産卵生態と養殖生簀網における虫卵分布 (2015)
  • Author(s): 平野千早, 石丸克也,白樫正
  • Organizer: 平成27年度日本魚病学会春期大会
  • Place of Presentation: 函館国際ホテル （北海道）
  • Year and Date: 2015-03-07 – 2015-03-08
• [Presentation] Shading of culture cage reduces the infection of skin fluke Neobenedenia girellae (2014)
  • Author(s): Shirakashi, S., Hirano, C., Fukushima, A.,Ogawa, K.
  • Organizer: The 13th meeting of International Congress of Parasitology
  • Place of Presentation: Camino Real Hotel Mexico City （メキシコ）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] ハダムシNeobenedenia girellaeの産卵とふ化の日周リズム (2014)
  • Author(s): 平野千早 白樫正 石丸克也
  • Organizer: 平成26年度日本魚病学会春期大会
  • Place of Presentation: 函館国際ホテル
• [Presentation] Development of a new method to reduce Neobenedenia girellae infection (2013)
  • Author(s): Hirano, Chihaya Shirakashi, Sho
  • Organizer: Aquaculture conference: To the Next 40 Years of Sustainable Global Aquaculture
  • Place of Presentation: Las Palmas, Gran Canaria
• [Presentation] ハダムシNeobenedenia girellaeの交接行動
  • Author(s): 小川和夫 白樫正 石谷浩江
  • Organizer: 平成25年度日本魚病学会秋期大会
  • Place of Presentation: 三重大学