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Identification of the biosignal factor induced by oral administration of recombinant WSSV proteins in kuruma shrimp.

Research Project

Project/Area Number 25450291
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionJapan Fisheries Research and Education Agency

Principal Investigator

Satoh Jun  国立研究開発法人水産総合研究センター, 増養殖研究所, 主任研究員 (10443350)

Co-Investigator(Renkei-kenkyūsha) MEKATA TOORU  国立研究開発法人 水産総合研究センター, 増養殖研究所, 研究員 (40597944)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsクルマエビ / ウイルス / 生体防御 / 水産増殖 / WSSV / PAV / ワクチン / ホワイトスポット病 / 魚病
Outline of Final Research Achievements

The 10 amino acid sequences of N-terminal were decided by proteomic analysis from vaccinated kuruma shrimp with recombinant WSSV proteins. Furthermore, amino acid sequences of this immune response factor were recognized by transcriptome analysis with gene expression of total RNA from some tissue of vaccinated shrimp, and it was estimated that belonged to the lectin family. And full sequence of this factor was decided by gene expression analysis according to each organization onset of vaccinated shrimp. These results were suggested that this factor controlled one end of quasi immune response in vaccinated recombinant WSSV protein kuruma shrimp.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report

Research Products

(3 results)

All 2016 2015 2014

All Presentation (2 results) Book (1 results)

  • [Presentation] クルマエビWSSV感染経路推定のためのウイルス遺伝子領域の特定2016

    • Author(s)
      浜野かおる・阿部 啓美・佐藤 純
    • Organizer
      平成28年度日本魚病学会春季大会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 天然クルマエビのWSSV(=PRDV)の保有状況とORF解析に基づく感染経路の推定2014

    • Author(s)
      佐藤 純
    • Organizer
      日本魚病学会
    • Place of Presentation
      函館国際ホテル
    • Related Report
      2013 Research-status Report
  • [Book] 水産学シリーズ181「ハタ科魚類の水産研究最前線」2015

    • Author(s)
      森 広一郎,佐藤 純,米加田 徹
    • Total Pages
      157
    • Publisher
      恒星社厚生閣
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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