• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Age-related changes in ovarian gene expression in Pacific bluefin tuna

Research Project

Project/Area Number 25450294
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionJapan Fisheries Research and Education Agency

Principal Investigator

GEN KOICHIRO  国立研究開発法人水産総合研究センター, その他部局等, 研究員 (80372051)

Co-Investigator(Kenkyū-buntansha) KAZETO Yukinori  水産総合研究センター, 増養殖研究所, 主任研究員 (60399996)
MEKUCHI Miyuki  水産総合研究センター, 中央水産研究所, 研究員 (40594007)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsクロマグロ / 初回成熟 / 成熟誘導遺伝子 / 卵巣 / 繁殖生理 / 水産増養殖
Outline of Final Research Achievements

An understanding of the mechanism underlying puberty is important for establishment of tuna farming. In this study, we aim to characterize reproductive-related genes in Pacific bluefin tuna and to examine changes in their expression levels during the pubertal to adulthood period in ovary. Using standard cloning technique, we have obtained 50 candidate genes for reproductive traits in bluefin tuna ovary. Comparative analysis of gene expression revealed that transcripts encoding steroid hormone synthase and receptors (PR, AR) of pubertal female were significantly higher than that of adult female. In contrast, adult fish showed significantly higher levels of ovary ARID and CREB mRNA and circulating estrogens. These results suggested that age-related changes in reproductive-related genes expression may result from alterations in ovarian development characteristics and serum estrogens in pubertal female.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 Other

All Presentation (2 results) Book (2 results)

  • [Presentation] 養殖クロマグロの加齢に伴う繁殖特性の変化2015

    • Author(s)
      玄浩一郎、馬久地みゆき、風藤行紀、二階堂英城
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Year and Date
      2015-03-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Evaluation of seasonal variation in reproduction-related gene expression in the pacific bluefin tuna Thunnus orientalis using nest-generation sequencing

    • Author(s)
      Mekuchi, M., Yasuike, M., Ojima, N., Gen, K., Sugaya, T., Nikaido, H., Shimizu, S.
    • Organizer
      International Plant and Animal Genomics
    • Place of Presentation
      San Diego, CA, USA
    • Related Report
      2013 Research-status Report
  • [Book] Biology and Ecology of Bluefin Tuna2015

    • Author(s)
      Gen K.
    • Total Pages
      30
    • Publisher
      CRC Press
    • Related Report
      2015 Annual Research Report
  • [Book] マグロの資源と生物学2014

    • Author(s)
      松原孝博、玄浩一郎、澤口小有美、持田和彦、二階堂英城
    • Total Pages
      13
    • Publisher
      成山堂
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi