Effect of marine foods on intetstinal environment -Analysis with FLX-pyrosequence method

• Project/Area Number: 25450300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Takashi Kuda 東京海洋大学, その他部局等, 准教授 (00290081)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 腸内フローラ / 海藻 / アルギン酸 / ラミナラン / サルモネラ / 魚肉 / 腸内腐敗産物 / 腸内発酵 / 褐藻類

Outline of Final Research Achievements

Effect of water soluble polysaccharide in brown algae, alginate and laminaran, on cecal environment in rats were examined.These polysaccharides fermented by the cecal microbiota and suppressed putrefactive compounds.
Differences of the effects of milk casein, soyprotein and fish-meal on the cecal environment were examined. Compared with milk casein, putrefactive compunds were increased by soyprotein and fish-meal. However, feeding of algae improved the cecal environment in the rats fed soyprotein and fish meal.
Furthermore, alginate in diet suppressed infection of Salmonella in rats.

Research Products

• [Journal Article] Effect of sodium-alginate and laminaran on Salmonella Typhimuriuminfection in human enterocyte-like HT-29-Luc cells and BALB/c mice (2015)
  • Author(s): Ｋｕｄａ， Ｔ．, Ｋｏｓａｋａ， Ｍ．, Hirano, S., Kawahara, M., Sato, M., Kaneshima, T., Nishizawa, M., Nishizawa, M., Takahashi, H., Kimura, B.
  • Journal Title: Carbohydrate Polymers Volume: 125 Pages: 113-119
  • DOI: 10.1016/j.carbpol.2015.01.078
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Caecal fermentation, putrefaction and microbiotas in rats fed milk casein, soy protein or fish meal (2014)
  • Author(s): Choa、A., Kuda, T., Yazaki, T., Takahashi, T., Kimura, B.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 98 Issue: 6 Pages: 2779-2787
  • DOI: 10.1007/s00253-013-5271-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Caecal environment of rats fed far East Asian-modelled diets (2014)
  • Author(s): Choa、A., Kuda, T., Yazaki, T., Takahashi, T., Kimura, B.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 98 Issue: 10 Pages: 4701-4709
  • DOI: 10.1007/s00253-014-5535-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FLX pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas (2013)
  • Author(s): An, C., Kuda, T., Yazaki, T., Takahashi, H., Kimura, B
  • Journal Title: Appled and Environmental Microbiology Volume: 79 Issue: 3 Pages: 860-866
  • DOI: 10.1128/aem.02354-12
  • Peer Reviewed
• [Journal Article] Diet-induced changes in alginate- and laminaran-fermenting bacterial levels in the caecal contents of rats (2013)
  • Author(s): An, C., Yazaki, T., Takahashi, H., Kuda, T., & Kimura, B
  • Journal Title: Journal of Functional Foods Volume: 5 Issue: 1 Pages: 389-394
  • DOI: 10.1016/j.jff.2012.11.011
  • Peer Reviewed
• [Presentation] 高大豆たん白質食ラットの盲腸内フローラに及ぼす褐藻類多糖類の影響 (2015)
  • Author(s): 中田徹・高橋肇・木村凡・久田孝
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 仙台市
  • Year and Date: 2015-09-24
• [Presentation] 大豆たん白質食ラットの腸内腐敗産物産生に及ぼすラミナランおよびアルギン酸の影響 (2014)
  • Author(s): 中田徹・安チョア・高橋肇・木村凡・久田孝「
  • Organizer: 第１９回日本食物繊維学会
  • Place of Presentation: 大妻女子大学
  • Year and Date: 2014-11-29 – 2014-11-30