Molecular mechanism of the defense systems against host immunity by causative bacillus of Edwardsiellosis in fishes - Cell biological approach -
| Project/Area Number |
25450304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Nagasaki University |
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Principal Investigator |
OSATOMI Kiyoshi 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40253702)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Kenji 長崎大学, 水産・環境科学総合研究科(水産), 教授 (10039737)
KANAI Kinya 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40145222)
ODA Tatsuya 長崎大学, 水産・環境科学総合研究科(水産), 教授 (60145307)
YOSHIDA Asami 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (80589870)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | flagellin / 抗酸化酵素 / プロモーター / 魚病細菌 / 酸化ストレス / 細胞培養系 |
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| Outline of Final Research Achievements |
In this study, we examined the effects of extracellular products (ECP) from Edwardsiella tarda (E.tarda) strains and recombinant flagellin from virulent strain of E.tarda on macrophages in terms of the induction of TNF-α and NO. Notably, recombinant flagellin from virulent strain of E.tarda stimulated macrophages to induce TNF-α production. From the deletion analysis of the SOD1 promoter, it was suggested that NF-IL6 binding site was the regulatory element of SOD1 against the oxidative stress.
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Report
(4 results)
Research Products
(4 results)
