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Role of cell-mediated immunity in the protection against nocardiosis and mycobacteriosis in fish

Research Project

Project/Area Number 25450305
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionKagoshima University

Principal Investigator

Araki Kyosuke  鹿児島大学, 農水産獣医学域水産学系, 助教 (30409073)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsノカルジア症 / ミコバクテリア症 / 細胞性免疫 / ワクチン / ブリ / カンパチ / IL-12 / IFNgamma / Interleukin-12 / Interferon gamma / Interleukin-10 / 細胞内寄生細菌 / ミコバクテリウム症 / アジュバント / 結節
Outline of Final Research Achievements

In this project, we showed 1) interferon-γ and interleukin-12 which are related to the cell-mediated immunity, play a crucial role in the protection against Nocardia seriolae infection in amberjack, 2) interleukin-10 is related to induction of humoral immune response against Nocardia seriolae infection, 3) IL-12 is a promising adjuvant for inactivated vaccine against Nocardiosis in amberjack, 4) 6 kDa early secretory target (ESAT-6) and 10 kDa culture filtrate protein (CFP-10) from Mycobacterium sp. have a potential for the protective antigen against Mycobacterium sp. infection in fish.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Recombinant sialidase NanA (rNanA) cleaves alpha2-3 linked sialic acid of host cell surface N-linked glycoprotein to promote Edwardsiella tarda infection2015

    • Author(s)
      Chigwechokha, P.K., M. Tabata, S. Shinyoshi, K. Oishi, K. Araki, M. Komatsu, T. Itakura, K. Shiozaki
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 47 Issue: 1 Pages: 34-45

    • DOI

      10.1016/j.fsi.2015.08.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of Edwardsiella tarda in ginbuna crucian carp (Carassius auratus langsdorfii)2015

    • Author(s)
      Yamasaki, M., K. Araki, K. Maruyoshi, M. Matsumoto, C. Nakayasu, T. Moritomo, T. Nakanishi, A. Yamamoto
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 45 Issue: 2 Pages: 437-442

    • DOI

      10.1016/j.fsi.2015.04.038

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role of CD4+ and CD8α+ T cells in protective immunity against2014

    • Author(s)
      Yamasaki M, Araki K, Nakanishi T, Nakayasu C, Yamamoto A.
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 36 Issue: 1 Pages: 299-304

    • DOI

      10.1016/j.fsi.2013.11.016

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serum GlucNAc-binding IgM of fugu (Takifugu rubripes) suppresses2013

    • Author(s)
      Tsutsui, S., Ariji, T., Sato, A., Yoshida, T., Yamamura, N., Odaka, T., Araki, K., Suetake, H., Miyadai, T., Nakamura, O.
    • Journal Title

      Dev. Comp. Immunol.

      Volume: 41 Issue: 1 Pages: 20-26

    • DOI

      10.1016/j.dci.2013.03.018

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] カンパチのInterleukin-10による細胞性免疫の抑制2016

    • Author(s)
      松本萌・Mahmoud Tanekhy Amer・早志和真・西谷篤・今岡慶明・柳宗悦・荒木亨介・山本淳
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 魚類ミコバクテリア症原因細菌が産生するESAT-6およびCFP-10の細胞性免疫誘導能の解析2016

    • Author(s)
      早志和真・松本萌・中西照幸・荒木亨介・山本淳
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ブリ類のInterferon-γ遺伝子の単離および機能解析2015

    • Author(s)
      松本萌・荒木亨介・早志和真・下野友未・劉倩・柳宗悦・今岡慶明・山本淳
    • Organizer
      平成27年度日本水産学会九州支部大会
    • Place of Presentation
      宮崎大学(宮崎県宮崎市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] 魚類肝臓におけるシアリダーゼNeu1の意義2015

    • Author(s)
      高瀬諒・龍薗せな・若松麗菜・Petros Kingstone Chigwechokha・本田晃伸・荒木亨介・小松正治・塩崎一弘
    • Organizer
      平成27年度日本水産学会九州支部大会
    • Place of Presentation
      宮崎大学(宮崎県宮崎市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] E. tardaの感染予防を目的としたシアリダーゼNanA阻害物質の探索2015

    • Author(s)
      新吉沙弥香、若松麗菜、Petros Kingstone Chigwechokha、松崎光輝、荒木亨介、小松正治、塩崎一弘
    • Organizer
      平成27年度日本水産学会九州支部大会
    • Place of Presentation
      宮崎大学(宮崎県宮崎市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] カンパチのノカルジア症不活化ワクチンにおけるInterleukin-12のアジュバント効果2015

    • Author(s)
      松本萌・荒木亨介・丸吉浩太・早志和真・末武弘章・柳宗悦・今岡慶明・山本淳
    • Organizer
      平成27年度日本魚病学会秋季大会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ブリ類の抗酸菌症不活化ワクチン接種後の免疫応答2015

    • Author(s)
      荒木亨介・下野友未・丸吉浩太・松本萌・早志和真・山崎雅俊・柳宗悦・今岡慶明・山本淳
    • Organizer
      平成27年度日本魚病学会秋季大会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Edwardsiella tarda感染における宿主細胞のN型糖タンパク糖鎖構造変化の重要性2015

    • Author(s)
      塩崎一弘・Petros Chigwechokha・新吉沙弥香・大木麗菜・荒木亨介・小松正治
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Edwardsiella tarda感染による脱シアリル化機構とその意義2015

    • Author(s)
      Petros Chigwechokha・新吉沙弥香・本田晃伸・大木麗菜・荒木亨介・小松正治・宮城妙子・塩崎一弘
    • Organizer
      第34回日本糖質学会年会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-08-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] カンパチInterleukin-12の機能解析2015

    • Author(s)
      松本萌、荒木亨介、早志和真、丸吉浩太、劉倩、末武弘章、柳宗悦、今岡慶明、山本淳
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2015-03-27 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] SMART理論による魚病細菌の選択培地の設計2015

    • Author(s)
      北野弘、荒木亨介、山本淳
    • Organizer
      平成27年度日本魚病学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2015-03-07 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] カンパチInterleukin12遺伝子の単離および発現解析2014

    • Author(s)
      松本萌、荒木亨介、丸吉浩太、山口愉生也、渡邊勇歩、早志和真、劉倩、柳宗悦、山本淳
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2014-09-19 – 2014-09-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 魚類のホルマリン不活化ワクチンによる液性免疫誘導と細胞性免疫抑制2014

    • Author(s)
      山崎雅俊、荒木亨介、中西照幸、中易千早、松﨑吾朗、山本淳
    • Organizer
      日本比較免疫学会第26回学術集会・第25回日本生体防御学会学術総会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2014-07-09 – 2014-07-11
    • Related Report
      2014 Research-status Report
  • [Presentation] ギンブナのEdwardsiella tardaホルマリン不活化ワクチンおよび弱毒生ワクチンにより誘導される二次免疫応答の比較2014

    • Author(s)
      山崎雅俊、荒木亨介、増田鷹侑、中西照幸、中易千早、山本淳
    • Organizer
      平成26年度日本魚病学会春季大会
    • Place of Presentation
      函館国際ホテル
    • Related Report
      2013 Research-status Report
  • [Presentation] N-acetyl-D-glucosamine-binding immunoglobulin M of fugu (Takifugu rubripes) inhibits the growth of fish pathogenic bacteria: A novel function of teleost antibody.2013

    • Author(s)
      Tsuitsui, S., Ariji, T, Sato, A., Yoshida, T., Yamamura, N., Odaka, T., Araki, K., Suetake, H., Miyadai, T., Nakamura, O.
    • Organizer
      First International Conference of Fish and Shellfish Immunology
    • Place of Presentation
      Vigo, Spain
    • Related Report
      2013 Research-status Report
  • [Presentation] Comparative analysis of adaptive immune response to live and inactivated Edwardsiella tarda in ginbuna crucian carp, Carassius auratus langsdorfii.2013

    • Author(s)
      Yamasaki, M., Araki, K., Nakanishi, T., Nakayasu, C., Yamamoto, A.
    • Organizer
      16th International Conference on Disease of Fish and Shellfish
    • Place of Presentation
      Tampere, Finland
    • Related Report
      2013 Research-status Report
  • [Presentation] Adaptive immune response in yellowtail Seriola quinqueradiata induced by formalin-killed Mycobacterium sp. in oil-adjuvant.2013

    • Author(s)
      Araki, K., Shimono, Y., Yamasaki, M., Matsumoto, S., Yanagi, S., Maeno, K., Yamamoto, A.
    • Organizer
      16th International Conference on Disease of Fish and Shellfish
    • Place of Presentation
      Tampere, Finland
    • Related Report
      2013 Research-status Report
  • [Presentation] ギンブナTh1様細胞のEdwardsiella tardaに対する感染防御における役割2013

    • Author(s)
      山崎雅俊、荒木亨介、中西照幸、中易千早、山本淳
    • Organizer
      平成25年度日本魚病学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ギンブナのヘルパーT細胞および細胞障害性T細胞はEdwardsiella tarda感染に対する防御免疫において重要な役割を果たす2013

    • Author(s)
      山崎雅俊、荒木亨介、中西照幸、中易千早、山本淳
    • Organizer
      平成25年度日本水産増殖学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 魚類用ワクチンアジュバント2016

    • Inventor(s)
      荒木亨介、松本萌、末武弘章
    • Industrial Property Rights Holder
      鹿児島大学、福井県立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-060775
    • Filing Date
      2016-03-24
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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