Structure and function of MytiLec-1, an alpha-galactose-binding lectin from the mussel Mytilus galloprovincialis.
Project/Area Number |
25450306
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Aquatic life science
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Research Institution | Yokohama City University |
Principal Investigator |
Ozeki Yasuhiro 横浜市立大学, 生命ナノシステム科学研究科, 教授 (70275022)
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Research Collaborator |
Imtiaj Hasan 横浜市立大学, 大学院, 博士後期課程
Koide Yasuhiro 横浜市立大学, 大学院, 博士後期課程
Kawsar S. M. Abe JSPS, 外国人招へい研究者
Fujii Yuki 長崎国際大学, 薬学部, 助教
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | レクチン / MytiLec-1 / MytiBase / トランスクリプト―ム / 海産無脊椎動物 / ムラサキイガイ / α-ガラクトース / 細胞内情報伝達 / 海洋無脊椎動物 / ガラクトース / パターン認識受容体 / 情報伝達 / アポトーシス / 微生物増殖抑制 / グロボトリオース / b-トレフォイル構造 / バーキットリンパ腫細胞 / 二枚貝 / 棘皮動物 / 細胞増殖 / 国際情報交換 Bangladesh |
Outline of Final Research Achievements |
Whole structural information including the gene, mRNA (GenBank LC125182) and 3-D structures (PDB 3wu and 3wv) of MytiLec-1, an alpha-galactose-binding lectin from the Mediterranean mussel Mytilus galloprovincialis have been provided by transcriptome, cloning and X-ray crystallographic analysis, respectively in addition to the primary structure (B3EWR1). Structural properties of MytiLec-1 have two exons in the gene, alternative-N-terminal extension in the full-length mRNA and five essential amino acids in subunit of the polypeptide. MytiLec-1 multiply activated both classic MAP-kinase (MEK/ERK) and stress activated kinase (JNK and p38) pathways in addition to the activation of caspase-9/3. The potency of MytiLec-1 suggested that the lectin has a possibility to act as a pattern recognition receptor which induce cellular regulations of the mussel cells.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Purification and characterization of a novel chitinase from Trichosanthes dioica seed with antifungal activity2016
Author(s)
Syed Rashel Kabir, Musfikur Rahmana, Shahnim, Tasnim, Rezaul Karim, Nazma Khatun, Imtiaj Hasan, Ruhul Amin, Shaikh Shohidul Islam, Nurujjaman, Ahmad Humayun Kabir, Niranjan Kumar Sana, Yasuhiro Ozeki, A.K.M. Asaduzzaman
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Journal Title
International Journal of Biological Macromolecules
Volume: 84
Pages: 62-68
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] MytiLec, a mussel R-type lectin, interacts with surface glycan Gb3 on Burkitt’s lymphoma cells to trigger apoptosis through multiple pathways2015
Author(s)
Imtiaj Hasan, Shigeki Sugawara, Yuki Fujii, Yasuhiro Koide, Daiki Terada, Naoya Iimura, Toshiyuki Fujiwara, Keisuke G. Takahashi, Nobuhiko Kojima, Sultana Rajia, Sarkar M. A. Kawsar, Robert A. Kanaly, Hideho Uchiyama, Masahiro Hosono, Yukiko Ogawa, Hideaki Fujita, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki
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Journal Title
Marine Drugs
Volume: 13
Issue: 12
Pages: 7377-7389
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Chemically modified uridine molecules incorporating acyl residues to enhance antibacterial and cytotoxic activities2015
Author(s)
Sarkar M. A. Kawsar, Hamida A. Ara, Sheikh Aftab Uddin, Mohammed K. Hossain, Shagir A. Chowdhury, Abul F. M. Sanaullah, Mohammad A. Manchur, Imtiaj Hasan, Yukiko Ogawa, Yuki Fujii, Yasuhiro Koide, Yasuhiro Ozeki
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Journal Title
International Journal of Organic Chemistry
Volume: 5
Issue: 04
Pages: 232-245
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A galactose-binding lectin isolated from Aplysia kurodai (sea hare) eggs Inhibits streptolysin-induced hemolysis2015
Author(s)
Hasan I, Watanabe M, Ishizaki N, Sugita-Konishi Y, Kawakami Y, Suzuki J, Dogasaki C, Rajia S, Kawsar SMA, Koide Y, Kanaly RA, Sugawara S, Hosono M, Ogawa Y, Fujii Y, Iriko H, Hamako J, Matsui T, Ozeki Y
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Journal Title
Molecules (Basel)
Volume: 19(9)
Issue: 9
Pages: 13990-14003
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] MytiLec, an R-type lectin from Mediterranean mussel regulates cancer cell growth through glycan-dependent signaling.2015
Author(s)
Hasan I, Fujii Y, Ogawa Y, Sugawara S, Hosono M, Koide Y, Rajia S, Kawsar S M A, Kanaly R A, Fujiwara T, Fujita H, Ozeki Y.
Organizer
23rd International Glycoconjugates Symposium (GLYCO23)
Place of Presentation
Sprit, Croatia
Year and Date
2015-09-15
Related Report
Int'l Joint Research
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[Presentation] Purification of novel a-galactoside-binding lectins from aquatic animals and their distinct cell regulatory activities.2013
Author(s)
Hasan I, Koide Y, Kawsar SMA, Matsumoto R, Fujii Y, Ogawa Y, Sugawara S, Hosono M, Nitta K, Ozeki Y.
Organizer
22nd International Symposium on Glycoconjugates
Place of Presentation
Dalian, China
Related Report
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[Book] Marine Glycobiology, Principles and Applications. Se-Kwon Kim ed. Chapter 33 "Structural glycobiology for lectin to promote advanced biomedical research" ISBN: 97814987096132016
Author(s)
Hasan I, Fujii Y, Rajia S, Kawsar S M A, Sugawara S, Ogawa Y, Hosono M, Koide Y, Kanaly R A, Ozeki Y.
Total Pages
15
Publisher
CRC press (Taylor & Francis Group)
Related Report
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[Book] Marine OMICS, Principles and Applications. Se-Kwon Kim ed. Chapter 21 "SUEL/RBL-lectins and their biomedical applications" ISBN 97814822582022016
Author(s)
Koide Y, Hasan I, Fujii Y, Kawsar S M A, Rajia S, Sugawara S, Ogawa Y, Hosono M, Kanaly R A, Ozeki Y.
Total Pages
11
Publisher
CRC press (Taylor & Francis Group)
Related Report
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