| Project/Area Number |
25450306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Yokohama City University |
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Principal Investigator |
Ozeki Yasuhiro 横浜市立大学, 生命ナノシステム科学研究科, 教授 (70275022)
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| Research Collaborator |
Imtiaj Hasan 横浜市立大学, 大学院, 博士後期課程
Koide Yasuhiro 横浜市立大学, 大学院, 博士後期課程
Kawsar S. M. Abe JSPS, 外国人招へい研究者
Fujii Yuki 長崎国際大学, 薬学部, 助教
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | レクチン / MytiLec-1 / MytiBase / トランスクリプト―ム / 海産無脊椎動物 / ムラサキイガイ / α-ガラクトース / 細胞内情報伝達 / 海洋無脊椎動物 / ガラクトース / パターン認識受容体 / 情報伝達 / アポトーシス / 微生物増殖抑制 / グロボトリオース / b-トレフォイル構造 / バーキットリンパ腫細胞 / 二枚貝 / 棘皮動物 / 細胞増殖 / 国際情報交換 Bangladesh |
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| Outline of Final Research Achievements |
Whole structural information including the gene, mRNA (GenBank LC125182) and 3-D structures (PDB 3wu and 3wv) of MytiLec-1, an alpha-galactose-binding lectin from the Mediterranean mussel Mytilus galloprovincialis have been provided by transcriptome, cloning and X-ray crystallographic analysis, respectively in addition to the primary structure (B3EWR1). Structural properties of MytiLec-1 have two exons in the gene, alternative-N-terminal extension in the full-length mRNA and five essential amino acids in subunit of the polypeptide. MytiLec-1 multiply activated both classic MAP-kinase (MEK/ERK) and stress activated kinase (JNK and p38) pathways in addition to the activation of caspase-9/3. The potency of MytiLec-1 suggested that the lectin has a possibility to act as a pattern recognition receptor which induce cellular regulations of the mussel cells.
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