Elucidation of the important IL-2/15 /15L cytokine family functions in the fish immune system
| Project/Area Number |
25450310
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
中西 照幸 日本大学, 生物資源科学部, 教授 (00322496)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Fish / Immunology / Evolution / Cytokine / Interleukin 2 / Interleukin 15 / Interleukin 15-like / Receptor / fish / cytokine / interleukin / evolution / IL-2 / IL-15 / IL-15L / IL-5 / IL-15-like / Interleukin 15L |
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| Outline of Final Research Achievements |
For IL-2/15/15L cytokines we found that the trout cytokines IL-2, IL-15, and IL-15L cross-react with IL-15Ra across fish-mammal borders, and vice versa, except for mammalian IL-2 which only binds mammalian IL-2Ra. We found that trout cytokines IL-2, IL-15, and IL-15L are N-glycosylated and have functional effects on trout lymphocytes, and that they can signal through chimera receptor chains on co-transfected cells which consist of rainbow trout IL-2Rb, IL-21Ra or IL-2Rg ectodomains that were combined with the transmembrane + cytoplasmic domains of the corresponding bovine receptor chains. We are currently trying to repeat the effect by adding exogenous instead of co-transfected trout cytokines. In addition, we found that recombinant carp IL-2 can help with the establishment of permanent T cell cultures. Furthermore, we were the first to find bona fide genes of the IL-5 cytokine family in teleost fish.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Identification of a gene for an ancient cytokine, interleukin 15-like, in mammals; interleukins 2 and 15 co-evolved with this third family member, all sharing binding motifs for IL-15Rα.2014
Author(s)
Dijkstra JM, Takizawa F, Fischer U, Friedrich M, Soto-Lampe V, Lefèvre C, Lenk M, Karger A, Matsui T, Hashimoto K
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Journal Title
Immunogenetics 66:93-103
Volume: 66
Issue: 2
Pages: 93-103
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species.2014
Author(s)
Takizawa, F., Araki, K., Ohtani, M., Toda, H., Saito, Y., Lampe, V.S., Dijkstra, J.M., Ototake, M., Moritomo, T., and Nakanishi, T.
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Journal Title
Fish & shellfish immunology
Volume: 36
Issue: 1
Pages: 215-222
DOI
Related Report
Peer Reviewed / Open Access
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