Mode of action of androgenic gland on male reproduction in Marsupenaeus japonicus

• Project/Area Number: 25450311
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Japan Fisheries Research and Education Agency
• Principal Investigator: OKUMURA Takuji 国立研究開発法人水産総合研究センター, 増養殖研究所, 主幹研究員 (30372030)
• Co-Investigator(Kenkyū-buntansha): OOHIRA Tsuyoshi 神奈川大学, 理学部, 准教授 (10361809)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 内分泌 / 性分化 / 精子形成 / クルマエビ / 精巣 / 甲殻類 / 造雄腺ホルモン

Outline of Final Research Achievements

We investigated roles of androgenic gland hormone on male reproduction in kuruma prawn Marsupenaeus japonicus. The gene expression levels of androgenic gland hormone increased in accordance with the growth of male prawn. Bilateral removal of eyestalks from male prawn induced hypertrophy of androgenic gland cells and increase of testis weight. These results indicate that androgenic gland is under inhibitory control of eyestalk factor and stimulates testis development. Gene expression analysis revealed that expression levels of some genes increased in the hypertrophied testis of eyestalk-removed male prawns. This result suggests that the acceleration of androgenic gland hormone secretion by eyestalk removal stimulates expression of these genes.

Research Products

• [Presentation] クルマエビの繁殖生理 (2016)
  • Author(s): 奥村 卓二
  • Organizer: 平成28年度日本水産学会増殖懇話会第一回講演会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2016-03-26
• [Presentation] クルマエビ造雄腺は眼柄因子に抑制的に調節され精子形成促進に働いている (2015)
  • Author(s): 奥村 卓二
  • Organizer: 平成27年度日本水産学会秋季大会
  • Place of Presentation: 東北大学（仙台市）
  • Year and Date: 2015-09-24
• [Presentation] クルマエビ造雄腺ホルモン様分子の遺伝子発現の周年変化 (2014)
  • Author(s): 杉岡浩行、鶴岡慎哉、大平剛
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-21
• [Presentation] クルマエビ造雄腺の摘出技術の確立 (2013)
  • Author(s): 杉岡 浩行、鶴岡 慎哉、朝比奈 潔、石坂 紀子、大平 剛
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ
• [Presentation] Localization of an insulin-like androgenic gland factor (IAG) in the kuruma prawn Marsupenaeus japonicus (2013)
  • Author(s): 杉岡 浩行、鶴岡 慎哉、石坂 紀子、朝比奈 潔、大平 剛
  • Organizer: 17th International Congress of Comparative Endocrinology
  • Place of Presentation: Universitat de Barcelona