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Analysis of the maturational process caused by temperature stimuli using an adequate model fish, the mummichog

Research Project

Project/Area Number 25450312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionJapan Fisheries Research and Education Agency

Principal Investigator

Shimizu Akio  国立研究開発法人水産総合研究センター, 中央水産研究所, グループ長 (90371830)

Co-Investigator(Kenkyū-buntansha) TSUTSUI Kazuyoshi  早稲田大学, 教育・総合科学学術院, 教授 (20163842)
Co-Investigator(Renkei-kenkyūsha) MEKICHI Miyuki  国立研究開発法人水産総合研究センター, 中央水産研究所・水産遺伝子解析センター, 研究員 (40594007)
YASUIKE Motoshige  国立研究開発法人水産総合研究センター, 中央水産研究所・水産遺伝子解析センター, 研究員 (20604820)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマミチョグ / FSH / LH / GnRH / 成熟 / 水温 / 生殖腺刺激ホルモン / 生殖内分泌系 / 水温刺激
Outline of Final Research Achievements

To examine temperature effects on mummichog (Fundulus heteroclitus) reproduction, adult fish were kept under low (8C) or moderate (16C) temperature conditions. After 6 weeks, both females and males were fully matured in the 16C group, whereas maturity was unchanged in the 8C group. Amounts of FSHbeta-mRNA showed considerable value throughuout the experiment, and showed little difference between the two temperatures. Amounts of LHbeta-mRNA was very low at the beginning of the experiment, and showed very high values in the 16C group in the latter part of the experiment. Plasma FSH levels showed high values in the 16C group in the latter part of the experiment, while plasma LH levels did not show distinct changes. These results indicate that FSH and LH have different roles for stimulating the maturation caused by the elevation of water temperature.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Gonadotropin-inhibitory hormone in the cichlid fish Cichlasoma dimerus: structure, brain distribution and differential effects on the secretion of gonadotropins and growth hormone2016

    • Author(s)
      Yorio, M.P.D., Sirkin, D.I.P., Delgadin, T.H., Shimizu, A., Tsutsui, K., Somoza, G.M., Vissio, P.G.
    • Journal Title

      J. Neuroendocrinol.

      Volume: 28

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reproductive physiology of the mummichog Fundulus heteroclitus - an excellent experimental fish2014

    • Author(s)
      Akio Shimizu
    • Journal Title

      Aqua-BioScience Monographs

      Volume: 7 Issue: 3 Pages: 79-116

    • DOI

      10.5047/absm.2014.00703.0079

    • NAID

      40020222917

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression and localization of gonadotropic hormone subunits (Gpa, Fshb, and Lhb) in the pituitary during gonadal differentiation in medaka2014

    • Author(s)
      Tohru Kobayashi, Yoshifumi Horie, Akio Shimizu, Shinji Adachi
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] マミチョグFSH及びLHに対するGnRHアナログの作用2016

    • Author(s)
      大久保 誠・清水昭男
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] マミチョグFundulus heteroclitus GTHR免疫陽性細胞の発生及び雌の生殖年周期に伴う変化2015

    • Author(s)
      清水昭男
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] マミチョグFundulus heteroclitusにおけるGnRH-GnRHR系の機能解析2014

    • Author(s)
      大久保誠・清水昭男
    • Organizer
      日本水産学会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] マミチョグFundulus heteroclitusにおけるGnRH及びGTHの年周変化2014

    • Author(s)
      大久保誠・清水昭男
    • Organizer
      日本水産学会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] マミチョグGTH受容体の発現解析2014

    • Author(s)
      大久保 誠・清水昭男
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      北海道大学水産学部
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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