Quantitative Evaluation of Ecosystem/Species diversity using Carbon/Nitrogen Stable Isotope Ratio and Metagenome

• Project/Area Number: 25450367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Rural environmental engineering/Planning
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Mori Atsushi 国立研究開発法人農業・食品産業技術総合研究機構, 農村工学研究部門, 教授 (10414418)
• Co-Investigator(Kenkyū-buntansha): KOIZUMI Noriyuki 国立研究開発法人農業・食品産業技術総合研究機構, 農村工学研究部門・水利工学研究領域, 上級研究員 (60301222)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生物多様性 / 農業農村整備事業 / 生態系配慮 / モニタリング / 生態系の多様性 / 遺伝子の多様性 / 農業水路 / 生物多様性の評価 / 生物多様性評価

Outline of Final Research Achievements

We have concluded that Carbon/Nitrogen stable isotope ratios could be used for evaluation of ecosystem diversity, and metagenome for evaluation of species diversity. It was observed that distribution of carbon/nitrogen isotope ratio of fish population in irrigation and drainage facilities varied with environment. Variation of both ratios in habitats consisting of varied environment elements was wide. It was considered that variation of the ratios indicated ecosystem diversity. Metagenoe analysis was also illustrated the possibility of use for evaluation of species diversity because its capability was higher than previous cloning method. Adaptation of biological community estimation using metagenome analysis in irrigation and drainage facilities is expected to be a epoch-making method that provide us comprehensive information regarding species diversity.

Research Products

• [Journal Article] 農業水路に生息するミシシッピアカミミガメの 炭素・窒素安定同位体比 (2016)
  • Author(s): 森 淳，澤田英司，小出水規行，渡部恵司，竹村武士
  • Journal Title: 農業農村工学会論文集 Volume: 302
  • NAID: 220000164832
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Progress of Rural Aquatic Ecosystem Analysis using Stable Isotope Ratios in Japan (2015)
  • Author(s): Atsushi MORI
  • Journal Title: Japan Agricultural Research Quarterly Volume: ４９（１） Pages: 11-16
  • NAID: 130004886288
  • Peer Reviewed
• [Journal Article] 人為的撹乱を受けない水田環境における食物網と物質フロー (2014)
  • Author(s): 森 淳，森岡 伸介，小出水 規行，Bounsong Vongvichith，渡部 恵司
  • Journal Title: 農村工学研究所技報 Volume: 215 Pages: 131-140
  • NAID: 40020107850
  • Peer Reviewed
• [Journal Article] 河川生態系における流速の多様性がもたらす付着藻類の多様性 (2014)
  • Author(s): 森 淳
  • Journal Title: 水環境学会誌 Volume: 37 Pages: 97-100
• [Presentation] 水田や水路の生物はいったい何を食べているのか？流域としての水田生態系解析の難しさ (2015)
  • Author(s): 森淳,森岡伸介,渡部恵司,小出水規行
  • Organizer: 平成27年度春季水産学会水産環境保全委員会企画シンポジウム
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] 農村生態系の変質と生態系配慮の取り組み (2015)
  • Author(s): 森 淳
  • Organizer: 自然環境復元学会
  • Place of Presentation: 東京農工大学（東京都府中市）
  • Year and Date: 2015-02-09
  • Invited
• [Presentation] 炭素安定同位体比が表すニホンアカガエルの移動に対する圃場整備の影響
  • Author(s): 森 淳，渡部 恵司，小出水 規行，竹村 武士，西田 一也
  • Organizer: 平成25年度農業農村工学会大会講演会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
• [Presentation] カエル類保全に向けた研究の成果
  • Author(s): 森 淳
  • Organizer: 第22回日華農業水利技術検討会
  • Place of Presentation: 福華国際文教会館（中華民国台北市）
  • Invited