| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Cells of Clostridium butyricum, C. cellulovorans, C. perfringens were grown on GS medium containing 1% wheat straw powder, and the enhancing effect of starch addition on hydrogen production was investigated. Hydrogen production by all Closrtidia tested, derived from wheat straw, were reached 4-fold volume compared to the culture without starch addition.
Activities of amylases and cellulases from C. cellulovorans culture with starch addition were increased up to 19.2-fold than the culture without starch addition, however, degradation of holocellulose in wheat straw was not enhanced. These results strongly suggested that sugar metabolism of C. cellulovorans would be altered to high hydrogen-yielding state.
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