| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In this study, we cultivated C. roseus in an enclosed artificial lighting plant factory and tried to develop the light controlling technique to improve production of vinblastine which was widely used in cancer chemotherapy. Red light compared with blue light and a mixture of red light and blue light (red PPFD: blue PPFD = 1:1) promoted to the growth and the concentrations of the monomeric precursors of vindoline and catharanthine in leaves. Vinblastine was accumulated in the leaves of the plants irradiated with UV-A light for 5 d and its content was increased until UV-A light intensity reached 10 W/m2. Vinblastine content in the younger leaves was greater than that in the older leaves. In conclusion, we constructed cultivation methods for efficient vinblastine production, as follows. Firstly, C. roseus plants are grown under 150 micro mol/m2/s red light only for one month, and then these plants are irradiated to red light supplemented with UV-A light of 10 W/m2 for 7 d.
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