Research and development of the light controlling technique to produce periwinkle alkaloids high-efficiently
| Project/Area Number |
25450373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Agricultural environmental engineering/Agricultural information engineering
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| Research Institution | Tamagawa University |
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Principal Investigator |
KANEKO Keiko 玉川大学, 学術研究所, 教授 (50332599)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Kazumasa 大阪大学, 大学院薬学研究科, 教授 (30199062)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ニチニチソウ / アルカロイド / 植物工場 / 発光ダイオード / 赤色光 / UV-A光 / 光強度 / 環境制御 / UV-A / ビンブラスチン / 葉齢 / 老化 / UVカットフィルム / CrPRX1遺伝子 / ビンドリン / カタランチン |
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| Outline of Final Research Achievements |
In this study, we cultivated C. roseus in an enclosed artificial lighting plant factory and tried to develop the light controlling technique to improve production of vinblastine which was widely used in cancer chemotherapy. Red light compared with blue light and a mixture of red light and blue light (red PPFD: blue PPFD = 1:1) promoted to the growth and the concentrations of the monomeric precursors of vindoline and catharanthine in leaves. Vinblastine was accumulated in the leaves of the plants irradiated with UV-A light for 5 d and its content was increased until UV-A light intensity reached 10 W/m2. Vinblastine content in the younger leaves was greater than that in the older leaves. In conclusion, we constructed cultivation methods for efficient vinblastine production, as follows. Firstly, C. roseus plants are grown under 150 micro mol/m2/s red light only for one month, and then these plants are irradiated to red light supplemented with UV-A light of 10 W/m2 for 7 d.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Improvement of vinblastine production by controlling light conditions in Catharanthus roseus2016
Author(s)
Fukuyama, T., Ohashi-Kaneko, K., Hirata, K., Harada, K., Muraoka, M., Ono, E., Watanabe, H.
Organizer
8th International symposium on Light in Horticulture
Place of Presentation
Michigan, USA
Year and Date
2016-05-22
Related Report
Int'l Joint Research
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