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Development of methods to evaluate molecular characteristics of livestock spermatozoa by the detection of aberrant fertility-regulating factors

Research Project

Project/Area Number 25450394
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionKobe University

Principal Investigator

HARAYAMA HIROSHI  神戸大学, (連合)農学研究科(研究院), 教授 (30281140)

Co-Investigator(Kenkyū-buntansha) MURASE Tetsuma  岐阜大学, 応用生物科学部, 教授 (30303514)
FUKUSHIMA Moriyuki  兵庫県立農林水産技術総合センター, 北部農業技術センター, 主任研究員 (60463395)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords応用動物 / 畜産学 / 雄性繁殖能力 / 精子 / 人工授精 / ウシ / ブタ
Outline of Final Research Achievements

The aim of the present study was to develop a new method to evaluate sperm molecular characteristics for identifying AI-subfertile males of livestock. In this study, we succeeded in the development of the method to evaluate molecular characteristics of bull (Japanese Black bull) cryopreserved and freshly ejaculated spermatozoa by the observation of acrosomal tyrosine-phosphorylated proteins. Moreover, we also showed that outcomes of AI and in vitro fertilization can be predicted by this evaluation for the spermatozoa.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (28 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 6 results) Presentation (20 results) (of which Invited: 3 results)

  • [Journal Article] Involvement of cyclic AMP-dependent unique signaling cascades in the decrease of serine/threonine-phosphorylated proteins in boar sperm head2016

    • Author(s)
      Ayane Isono, Shunsuke Tate, Kazumi Nakamura-Mori, Taichi Noda, Sho Ishikawa and Hiroshi Harayama
    • Journal Title

      Theriogenology

      Volume: 85 Issue: 6 Pages: 1152-1160

    • DOI

      10.1016/j.theriogenology.2015.11.031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Individual differences in the distribution of sperm acrosome associated 1 proteins among male patients of infertile couples; possible their impacts on outcomes of conventional in vitro fertilization2016

    • Author(s)
      Kazumi Kishida, Hiroshi Harayama, Fuminori Kimura and Takashi Murakami
    • Journal Title

      Zygote

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Individual differences in the distribution of acrosomal tyrosine-phosphorylated proteins in epididymal and ejaculated spermatozoa of Japanese Black bulls2016

    • Author(s)
      Miyuki M. Arai, Kenta Minami, Yukari Ogura, Nagisa Otsuka, Shohei Hama, Hiroshi Harayama, Mitsuhiro Sakase and Moriyuki Fukushima
    • Journal Title

      Reproduction, Fertility and Development

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of acrosomal conditions of frozen-thawed spermatozoa on the results of artificial insemination in Japanese Black cattle2015

    • Author(s)
      Kazumi Kishida, Mitsuhiro Sakase, Kenta Minami, Miyuki M. Arai, Reiko Syoji, Namiko Kohama, Takayuki Akiyama, Akio Oka, Hiroshi Harayama and Moriyuki Fukushima
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 6 Pages: 519-524

    • DOI

      10.1262/jrd.2015-073

    • NAID

      130005113570

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct segment-specific functions of calyculin A-sensitive protein phosphatases in the regulation of cAMP-triggered events in ejaculated bull spermatozoa2015

    • Author(s)
      Yohei Mizuno, Ayane Isono, Aya Kojima, Miyuki M. Arai, Taichi Noda, Mitsuhiro Sakase, Moriyuki Fukushima and Hiroshi Harayama
    • Journal Title

      Molecular Reproduction and Development

      Volume: 82 Issue: 3 Pages: 232-250

    • DOI

      10.1002/mrd.22465

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Roles of extracellular Ca2+ in the occurrence of full-type hyperactivation in boar ejaculated spermatozoa pre-incubated to induce the cAMP-triggered events2015

    • Author(s)
      Aya Kojima, Yuki Matsushita, Yukari Ogura, Sho Ishikawa, Taichi Noda, Tetsuma Murase and Hiroshi Harayama
    • Journal Title

      Andrology

      Volume: 3 Issue: 2 Pages: 321-331

    • DOI

      10.1111/andr.12005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression patterns of the activator type of cAMP-responsive element modulator in testicular germ cells of Japanese Black bulls2014

    • Author(s)
      Taichi Noda, Kenta Minami, Aya Kojima, Yohei Mizuno, Ayane Isono, Mitsuhiro Sakase, Moriyuki Fukushima, Hiroshi Harayama
    • Journal Title

      Theriogenology

      Volume: 81 Issue: 8 Pages: 1012-1020

    • DOI

      10.1016/j.theriogenology.2014.01.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of Intracellular Cyclic AMP Signal Transduction in the Capacitation and Subsequent Hyperactivation of Mouse and Boar Spermatozoa2013

    • Author(s)
      Hiroshi Harayama
    • Journal Title

      Journal of Reproduction and Development

      Volume: 59 Issue: 5 Pages: 421-430

    • DOI

      10.1262/jrd.2013-056

    • NAID

      130003381850

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ブタ精子での先体反応誘起に伴う頭部タンパク質SPACA1の変化2016

    • Author(s)
      小倉 有香里,原山 洋
    • Organizer
      日本畜産学会第121回大会
    • Place of Presentation
      日本獣医生命大学(東京都・武蔵野市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ウシ新鮮射出精子における先体チロシンリン酸化タンパク質の分布状態2016

    • Author(s)
      荒井 美由紀,原山 洋
    • Organizer
      第4回関西生殖医学集談会・第48回関西アンドロロジーカンファレンス合同研究会
    • Place of Presentation
      ハービスPLAZA(大阪府・大阪市)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] ウシ新鮮射出精子の分子性状における個体差 -先体チロシンリン酸化タンパク質と人工授精成績との関係-2015

    • Author(s)
      荒井 美由紀,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学農学部(宮崎県・宮崎市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 人工授精成績・体外受精成績に影響を与える哺乳類精子の先体タンパク質2015

    • Author(s)
      原山 洋
    • Organizer
      第60回日本生殖医学会学術講演会 シンポジウム3 「受精に関する最近の話題」
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2015-04-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 哺乳類の精子学 -運動の開始から受精まで-2015

    • Author(s)
      原山 洋
    • Organizer
      第35回関西生殖発生毒性フォーラム 基礎教育講演
    • Place of Presentation
      弁天町 ORC200 生涯学習センター(大阪府・大阪市)
    • Year and Date
      2015-04-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ウシ凍結精子での先体損傷・離脱に伴うIZUMO1の変化2015

    • Author(s)
      福田 匡起,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮市 宇都宮大学
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 低繁殖症雄ウシの精子における欠陥型タンパク質の検出および特性解析2014

    • Author(s)
      原山 洋
    • Organizer
      受胎率向上SIG第1回会合
    • Place of Presentation
      東京 東京大学
    • Year and Date
      2014-12-20
    • Related Report
      2014 Research-status Report
  • [Presentation] The association between spatial distribution patterns of SPACA1 in human ejaculated sperms and outcomes of conventional IVF2014

    • Author(s)
      Kazumi Kishida, Hiroshi Harayama, Fuminori Kimura and Takashi Murakami
    • Organizer
      World Congress of Reproductive Biology 2014
    • Place of Presentation
      EICC, Edinburgh
    • Year and Date
      2014-09-02 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 黒毛和種精子でのチロシンリン酸化タンパク質の分布状態が先体の安定性に及ぼす影響2014

    • Author(s)
      荒井 美由紀,南 健太,小倉 有香里,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広市 帯広畜産大学
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] ブタ精子での先体反応に伴う頭部タンパク質「SPACA1」の分布および分子マスの変化2014

    • Author(s)
      小倉 有香里,高岸 佑樹,小島 彩,石川 翔,原山 洋
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広市 帯広畜産大学
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] ウシ凍結保存精子における先体の損傷・離脱が頭部でのIZUMO1の分布に及ぼす影響2014

    • Author(s)
      福田 匡起,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広市 帯広畜産大学
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] ウシ精巣におけるcAMP依存性転写調節因子CREMの発現パターンの解析2014

    • Author(s)
      野田 大地,南 健太,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広市 帯広畜産大学
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 家畜精子のハイパーアクチベーションに先立つ部位特異的なキャパシテーション2014

    • Author(s)
      原山 洋
    • Organizer
      日本アンドロロジー学会第33回学術大会 シンポジウム 1 「Capacitation の最前線を極める」
    • Place of Presentation
      軽井沢町 軽井沢プリンスホテルウエスト
    • Year and Date
      2014-06-12 – 2014-06-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Full-type hyperactivationの誘起に伴う家畜精子の頚部および中片部における変化2014

    • Author(s)
      原山 洋,水野 洋平,小島 彩
    • Organizer
      日本畜産学会第118回大会
    • Place of Presentation
      つくば市 つくば国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 人工授精で雌を受胎させにくい精子を産生する黒毛和種雄個体の検出法 -凍結保存後の精子先体の正常性と体内受精由来の移植可能胚率の関係-2013

    • Author(s)
      坂瀬 充洋,小路 玲子,小浜 菜美子,秋山 敬孝,岡 章生,原山 洋,福島 護之
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      府中市 東京農工大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ブタ精子におけるカルパインの検出および機能解析2013

    • Author(s)
      小島 彩,石川 翔,設楽 修,原山 洋
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      府中市 東京農工大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト射出精子におけるSPACA1の検出 -先体での検出パターンと体外受精成績との相関性-2013

    • Author(s)
      岸田 和美,原山 洋,木村 文則,村上 節
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      府中市 東京農工大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ウシ精子の鞭毛におけるタンパク質チロシンリン酸化および超活性化運動の誘起2013

    • Author(s)
      水野 洋平,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      関西畜産学会第63回大会
    • Place of Presentation
      彦根市 滋賀県立大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ブタ精子の鞭毛に分布するカルパイン2の役割

    • Author(s)
      小島 彩,原山 洋
    • Organizer
      第2回関西生殖医学集談会
    • Place of Presentation
      大阪市 ハービスPLAZA
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト射出精子の頭部におけるSPACA1の分布状態の個体差 -先体主部での分布状態と体外受精成績との関係-

    • Author(s)
      岸田 和美,原山 洋,木村 文則,村上 節
    • Organizer
      第2回関西生殖医学集談会
    • Place of Presentation
      大阪市 ハービスPLAZA
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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