| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Expression of chicken and turkey prolactin regulatory element binding protein in anterior pituitary gland was investigated during embryogenesis and reproductive cycle. Levels of PREB mRNA generally show the similar profile to PRL mRNA. Especially, high level of PREB mRNA was detected during incubation stage. Since high level PRL mRNA was induced by hypothalamic VIP during incubation stage, effects of VIP on mRNA expression of PREB in the anterior pituitary gland of embryo and laying stage were examined. Levels of PREB and PRL mRNAs were not changed by stimulation of VIP in the anterior pituitary gland of embryonic days of 14. However, induced effects of forskolin was observed in the embryonic day 14.On the other hands, stimulatory effects of VIP and forskolin were observed in the anterior pituitary gland of embryonic day 20. This result clearly indicating that effects of VIP mainly regulated by the mRNA levels of VIP receptor in the anterior pituitary gland.
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