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The role of N-terminal fragments generated by enzymatic cleavage of bovine placental lactogen

Research Project

Project/Area Number 25450404
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionIwate University

Principal Investigator

Takahashi Toru  岩手大学, 農学部, 教授 (20355738)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords胎盤 / ウシ / プロラクチン / 血管新生 / カテプシン / MMP / 胎盤性ラクトジェン / プロラクチン活性 / 妊娠
Outline of Final Research Achievements

Bovine placental lactogen (bPL) is a member of a placental prolactin (PRL) family that exhibits lactogenic activity. Bovine PL is a potential substrate for placental enzymes such as MMPs and cathepsins. These enzymes cleaved bPL and generated 25 kDa N-terminal fragments. Cathepsins and MMPs were detected in bovine placental conditioned medium (BPCM) and co-incubation of recombinant bPL with BPCM resulted in the generation of N-terminal bPL fragments. Lactogenic activity of intact bPL was ceased after enzymatic cleavage. Bioactivity for pro- and anti-angiogenesis of cleaved bPL was studied by cell proliferation assay using bovine brain vascular endothelial cells (BBMC). The cleaved bPL inhibited the proliferation of BBMC whereas intact bPL showed no significant effects in terms of BBMC proliferation. Anti-angiogenic activity of cleaved bPL might be shared in the regulation of bovine placental angiogenesis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (6 results)

  • [Journal Article] Effects of supplemented sericin on the development, cell number, cryosurvival, and number of lipid droplets in cultured bovine embryos2016

    • Author(s)
      Hosoe M, Inaba Y, Hashiyada Y, Imai K, Kajitani K, Hasegawa Y, Irie M, Teramoto H, Takahashi T, Niimura S.
    • Journal Title

      Animal Science Journal

      Volume: 87 Issue: 2 Pages: 241-247

    • DOI

      10.1111/asj.12628

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a programmable piggyback syringe pump and four-times- a-day injection regimen for superovulation in non-lactating Holstein cows2015

    • Author(s)
      Irshad AR, Sasaki T, Kubo T, Odashima N, Katano T, Osawa T, Takahashi T, Izaike Y.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Pages: 485-488

    • NAID

      130005104115

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ウシの繁殖研究の新展開2014

    • Author(s)
      高橋 透
    • Journal Title

      家畜診療

      Volume: 61 Pages: 267-273

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta.2013

    • Author(s)
      Hayashi KG, Hosoe M, Sakumoto R, Takahashi T.
    • Journal Title

      Reprod Biol Endocrinol.

      Volume: 11 Issue: 1 Pages: 62-62

    • DOI

      10.1186/1477-7827-11-62

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] RNA採血管によるウシの妊娠情報の収集と早期妊娠判定への適用2015

    • Author(s)
      吉野仁美、木崎景一郎、平田統一、山岸則夫、高橋透、居在家義昭、佐々木恒弥、橋爪一善
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学農学部
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 時間分解蛍光免疫測定法にによるウシ妊娠関連糖タンパク質2の測定系の構築2015

    • Author(s)
      和田夏海、会津満理奈、居在家義昭、高橋透
    • Organizer
      第109回日本繁殖生物学会
    • Place of Presentation
      宮崎大学農学部
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] カラードプラ超音波検査を用いた妊娠初期ウシ黄体の血流動態解析2015

    • Author(s)
      窪友瑛、金澤朋美、居在家義昭、高橋透
    • Organizer
      第109回日本繁殖生物学会
    • Place of Presentation
      宮崎大学農学部
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホルスタイン種受胚牛における黄体血流量と受胎性の関連について2015

    • Author(s)
      金澤朋美、窪友瑛、関元秀、石山敬貴、金田義之、居在家義昭、高橋透
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学獣医学部
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bovine placental lactogen is cleaved by matrix metalloproteinases and resulted 25k N-terminal fragments inhibit the proliferation of vascular endothelial cells2014

    • Author(s)
      Shobu Sasaki, Ken-Go Hayashi, Misa Hosoe, Keiichiro Kizaki, Kazuyoshi Hashizume and Toru Takahashi
    • Organizer
      World Congress of Reproductive Biology
    • Place of Presentation
      英国 エジンバラ市
    • Year and Date
      2014-09-03 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Bovine placental lactogen is cleaved by matrix metalloproteinases and resulted 25k N-terminal fragments inhibit the proliferation of vascular endothelial cells2014

    • Author(s)
      Shobu Sasaki, Ken-Go Hayashi, Misa Hosoe, Keiichiro Kizaki, Kazuyoshi Hashizume and Toru Takahashi
    • Organizer
      World Congress of Reproductive Biology
    • Place of Presentation
      連合王国 エジンバラ市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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