| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
The present study was conducted to identify an IgY receptor contributing IgY uptake into avian egg yolks. Analysis of comprehensive RNA sequence in chicken ovarian follicles showed expression of 9,848 transcripts. Three already known IgY receptors were included in these transcripts, but their expression levels were low. To proceed an efficient selection of candidate gene from whole transcripts, chicken or quail groups possessing novel IgY uptake ability were explored. IgY-deficient chickens subjected to brusectomy increased IgY uptakes into egg yolks by 3-fold, suggesting usefulness of this chickens model for comprehensive RNA sequencing analysis. No remarkable changes were observed in ability of IgY uptakes into egg yolks among six quail stains.
|
