| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The present study assessed the survival of E. coli in various positions of piled cow manure compost. Key substrates which support E. coli regrowth in compost and the microbial competitors in terms of substrates assimilation were analyzed. E. coli survived longer time on top surface of pile, while E. coli in internal parts of pile eliminated. E. coli strains survived longer time in compost were capable of growing in higher temperature relative to E. coli isolated from raw cow manure. The addition of glucose resulted in higher growth of E. coli in compost. However, the addition of corn powder or dead bacterial biomass did not increase the number of E. coli. Bacterial species which incorporate glucose, corn powder or dead bacterial biomass in compost were identified by using Stable isotope probing method.
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