Studies on survival and regrowth of Escherichia coli in cow manure compost
Project/Area Number |
25450415
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Animal production science
|
Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
HANAJIMA Dai 国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター・酪農研究領域, 上級研究員 (20414708)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | 堆肥 / 大腸菌 / 安定同位体標識 / 炭素基質 / SIP法 / 炭素源 / 温度 / REP-PCR |
Outline of Final Research Achievements |
The present study assessed the survival of E. coli in various positions of piled cow manure compost. Key substrates which support E. coli regrowth in compost and the microbial competitors in terms of substrates assimilation were analyzed. E. coli survived longer time on top surface of pile, while E. coli in internal parts of pile eliminated. E. coli strains survived longer time in compost were capable of growing in higher temperature relative to E. coli isolated from raw cow manure. The addition of glucose resulted in higher growth of E. coli in compost. However, the addition of corn powder or dead bacterial biomass did not increase the number of E. coli. Bacterial species which incorporate glucose, corn powder or dead bacterial biomass in compost were identified by using Stable isotope probing method.
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Report
(4 results)
Research Products
(3 results)