Study of virulence inhibitory factor in Toxoplasma gondii

• Project/Area Number: 25450419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: IGARASHI Makoto 帯広畜産大学, 原虫病研究センター, 教授 (60374766)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: トキソプラズマ / 病原性 / 慢性感染 / シスト

Outline of Final Research Achievements

This study had been conducted to elucidate the role of TgDPA molecule as a virulence inhibitory factor in Toxoplasma gondii. The findings are summarized below. 1) TgDPA overexpressing strain was successfully produced. 2) The crystal structure of the TgDPA molecule was determined, suggesting that the enzyme activity was lost during evolution. 3) The pathogenicity varied depending on the presence or absence of TgDPA. 4) Induction of TgDPA expression was inhibited by deletion of lactate dehydrogenase gene (LDH1). 5) TgDPA did not affect proliferation / differentiation of protozoa. 6) TgDPA molecule was involved in gene expression fluctuation during cyst formation. 7) We succeeded in producing a double knockout strain with TgDERA, paralog of TgDPA gene.

Research Products

• [Journal Article] Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis. (2017)
  • Author(s): Abdelbaset, A.E., Alhasan, H., Salman, D., Karram, M.H., Ellah Rushdi, M.A., Xuenan, X., Igarashi, M.
  • Journal Title: Exp Parasitol. Volume: 172 Pages: 1-4
  • DOI: 10.1016/j.exppara.2016.11.003
  • NAID: 120006390995
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Lactate dehydrogenase in Toxoplasma gondii controls virulence, bradyzoite differentiation, and chronic infection. (2017)
  • Author(s): Abdelbaset E. Abdelbaset, Barbara A. Fox, Mohamed H. Karram, Mahmoud R. Abd Ellah, David J. Bzik, Makoto Igarashi.
  • Journal Title: PLoS One Volume: 12 Issue: 3 Pages: e0173745-e0173745
  • DOI: 10.1371/journal.pone.0173745
  • NAID: 120006391009
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Serological Survey and Factors Associated with Toxoplasma gondii Infection in Domestic Goats in Myanmar (2016)
  • Author(s): Bawm Saw, Maung Wint Yi, Win Myat Yee, Thu May June, Chel Hla Myet, Khaing Tin Aye, Wai Soe Soe, Htun Lat Lat, Myaing Tin Tin, Tiwananthagorn Saruda, Igarashi Makoto, and Katakura Ken
  • Journal Title: Scientifica Volume: 2016 Pages: 1-4
  • DOI: 10.1155/2016/4794318
  • NAID: 120005725149
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and functional divergence of the aldolase fold in Toxoplasma gondii. (2015)
  • Author(s): Tonkin, M.L., Halavaty, A.S., Ramaswamy, R., Ruan, J., Igarashi, M., Ngô, H.M., Boulanger, M.J.
  • Journal Title: J. Mol. Biol. Volume: 427 Issue: 4 Pages: 840-852
  • DOI: 10.1016/j.jmb.2014.09.019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of Toxoplasma gondii glyoxalase 1 and evaluation of inhibitory effects of curcumin on the enzyme and parasite cultures. (2015)
  • Author(s): Youn-Kyoung Goo, Junya Yamagishi, Akio Ueno, Mohamad Alaa Terkawi, Gabriel Oluga Aboge, Dongmi Kwak, Yeonchul Hong, Dong-Il Chung, Makoto Igarashi, Yoshifumi Nishikawa and Xuenan Xuan.
  • Journal Title: Parasites & Vectors Volume: 8：654 Issue: 1 Pages: 1-8
  • DOI: 10.1186/s13071-015-1268-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalences of <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in Pet Rabbits in Japan (2014)
  • Author(s): Salman, D., Oohashi, E., Mohamed, A.E.E., El-Mottelib, A.E.A., Okada, T., Igarashi, M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 6 Pages: 855-862
  • DOI: 10.1292/jvms.13-0632
  • NAID: 130003391296
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] A novel dense granule protein, GRA22, is involved in regulating parasite egress in Toxoplasma gondii. (2013)
  • Author(s): Okada T, Marmansari D, Li ZM, Adilbish A, Canko S, Ueno A, Shono H, Furuoka H, Igarashi M.
  • Journal Title: Mol Biochem Parasitol. Volume: 189 Issue: 1-2 Pages: 5-13
  • DOI: 10.1016/j.molbiopara.2013.04.005
  • Peer Reviewed
• [Presentation] Analysis of theRoleof Toxoplasma gondiiLactate dehydrogenase during stage conversion using Knockout techniques. (2016)
  • Author(s): Abdelbaset Abdelbaset、五十嵐 慎
  • Organizer: 第８５回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-18
• [Presentation] トキソプラズマのシスト形成に寄与する因子の探索 (2016)
  • Author(s): 村田 優穂、高野 量、五十嵐 慎、加藤 健太郎
  • Organizer: 第８５回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-18
• [Presentation] Molecular characterization of Toxoplasma gondii ANK1 (2015)
  • Author(s): Abdelbaset E. Abdelbaset, Doaa Salman, Mohamed H. Karram, Mahmoud R. Abd Ellah, 齋木選射, 五十嵐慎
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Prevalence and genetic characterization of Toxoplasma gondii isolate from Tokachi subprefecture in Japan. (2014)
  • Author(s): Salman Doaa、大橋英二、Abdelbaset Abdelbaset E、五十嵐慎
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Novel bradyzoite-specific genes affect the virulence of Toxoplasma gondii (2014)
  • Author(s): Doaa Salman、Muller Compaore、Suphasawatt Puranaveja、岡田只士、五十嵐慎
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学
• [Presentation] Characterization of Toxoplasma gondii DPA knock out and over expressing parasites. (2013)
  • Author(s): Muller Compaore、Doaa Salman、Suphasawatt Puranaveja、岡田只士、五十嵐慎
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学