Pathological studies on newly recognized diseases associated with bovine viral diarrhea virus (BVDV) and retrospective survey for new BVDV-associated disease states

• Project/Area Number: 25450420
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Kobayashi Yoshiyasu 帯広畜産大学, 畜産学部, 教授 (20301971)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Kotaro 帯広畜産大学, 畜産学部, 准教授 (90455709)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 病理・病態 / 牛ウイルス性下痢症ウイルス / 持続感染 / ウイルス遺伝子 / PCR / 神経疾患 / 血管 / 牛ウイルス性下痢・粘膜病 / 糸球体 / 血栓 / 病理 / 中枢神経

Outline of Final Research Achievements

Bovine viral diarrhea virus (BVDV) infection is one of the most important causative agent for digestive tract disease. Intrauterine infection to the fetus without effective immune response causes persistent infection and this state related to various pathologic states. This research revealed the precise pathobiology of newly recognized diseases associated with BVDV persistent infection: 1) leukodystrophy characterized by severe gliosis and hypomyelinogenesis, and 2) systemic glomeruloid angiopathy characterized by intramural proliferation of vascular endothelial cells and smooth muscle cells. In the present research, we also examined whether BVDV persistent infection relate to other uncleared disease conditions, including vascular diseases with fibrinoid change which is one of the characteristic finding for BVDV infection, and central nervous system diseases. Unfortunately, however, we could not find new BVDV persistent infection-related disease.

Research Products

• [Journal Article] Periventricular leukomalacia in a neonatal calf (2016)
  • Author(s): Koyama K, Fujita R, Maezawa M, Fukumoto N, Horiuchi N, Inokuma H, Kobayashi Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 7 Pages: 1175-1177
  • DOI: 10.1292/jvms.16-0034
  • NAID: 130005253795
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A parallelized pumpless artificial placenta system significantly prolonged survival time in a preterm lamb model. (2016)
  • Author(s): Miura Y, Matsuda T, Usuda H, Watanabe S, Kitanishi R, Saito M, Hanita T, Kobayashi Y.
  • Journal Title: Artif Organs Volume: 40 Issue: 5 Pages: E61-E68
  • DOI: 10.1111/aor.12656
  • Peer Reviewed / Open Access
• [Journal Article] Detection of the nonsense mutation of OPA3 gene in Holstein Friesian cattle with dilated cardiomyopathy in Japan (2015)
  • Author(s): Horiuchi, N., Kumagai, D., Matsumoto, K., Inokuma, H., Furuoka, H. and Kobayashi, Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 10 Pages: 1281-1283
  • DOI: 10.1292/jvms.15-0150
  • NAID: 130005107601
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis (2015)
  • Author(s): Miura, S., Horiuchi, N., Matsumoto, K., Kobayashi, Y., Kawazu, S. I. and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 7 Pages: 883-887
  • DOI: 10.1292/jvms.14-0591
  • NAID: 130005089949
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Glomus tumor of the liver in a cow (2015)
  • Author(s): Horiuchi, N., Komagata, M., Shitamura, K., Chiba, C., Matsumoto, K., Inokuma, H., Matsui, T. and Kobayashi, Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 6 Pages: 729-732
  • DOI: 10.1292/jvms.14-0327
  • NAID: 130005085855
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves (2015)
  • Author(s): Kojima, Y., Chiba, S., Horiuchi, N., Kobayashi, Y. and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 5 Pages: 605-607
  • DOI: 10.1292/jvms.14-0578
  • NAID: 130005071880
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Optic pathway degeneration in Japanese black cattle (2015)
  • Author(s): Chiba, S., Funato, S., Horiuchi, N., Matsumoto, K., Inokuma, H., Furuoka, H. and Kobayashi, Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 2 Pages: 147-154
  • DOI: 10.1292/jvms.14-0299
  • NAID: 130004704139
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ホルスタイン種子牛にみられた小脳膿瘍の１症例. (2015)
  • Author(s): 中島永成, 二宮理沙, 福田茂夫, 藤井貴志, 堀内雅之, 古林与志安, 猪熊壽.
  • Journal Title: 獣医畜産新報 Volume: 68(1) Pages: 42-46
  • Peer Reviewed
• [Journal Article] ホルスタイン種乳牛における脳幹部腫瘍の1症例. (2014)
  • Author(s): 三浦沙織, 千葉史織, 高橋裕之, 福田茂夫, 藤井貴志, 新井鐘蔵, 古林与志安, 猪熊 壽.
  • Journal Title: 日獣会誌 Volume: 67(11) Pages: 850-853
  • NAID: 130004888050
  • Peer Reviewed
• [Journal Article] ホルスタイン種育成牛にみられた舌筋および横隔膜筋の変性を伴う心筋症の１症例. (2014)
  • Author(s): 三浦沙織, 熊谷大史郎, 堀内雅之, 下タ村圭市, 古林与志安, 猪熊壽.
  • Journal Title: 獣医畜産新報 Volume: 67(6) Pages: 445-448
  • Peer Reviewed
• [Journal Article] Utility of Serum Thymidine Kinase Activity Measurements for Cases of Bovine Leukosis with Difficult Clinical Diagnoses (2013)
  • Author(s): Tawfeeq, M. M., Miura, S. Horiuchi, N., Kobayashi, Y., Furuoka, H. and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 9 Pages: 1167-1172
  • DOI: 10.1292/jvms.12-0572
  • NAID: 130003362197
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] 牛ウイルス性下痢ウイルス感染に関連するミエリン形成不全により先天性小脳症状を呈したホルスタイン種子牛の5症例. (2013)
  • Author(s): 猪熊壽, 小嶋由夏, 千葉史織, 古林与志安, 高垣勝仁.
  • Journal Title: 産業動物臨床医誌 Volume: 4(1) Pages: 25-29
  • NAID: 130003379742
  • Peer Reviewed
• [Journal Article] Evaluation of Gene Expression in Peripheral Blood Cells as a Potential Biomarker for Enzootic Bovine Leukosis (2013)
  • Author(s): Tawfeeq, M. M., Horiuchi, N., Kobayashi, Y., Furuoka, H. and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 9 Pages: 1213-1217
  • DOI: 10.1292/jvms.13-0109
  • NAID: 130003362188
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] 牛の疾病：牛のアミロイド症と子牛の神経疾患の病理学 (2014)
  • Author(s): 古林与志安
  • Organizer: 平成25年度日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 千葉（幕張メッセ）
  • Invited