Development of vaccines against leishmaniasis using polyvalent CDV vaccine and cytokine adjuvant.

• Project/Area Number: 25450421
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: SATO HIROKI 東京大学, 医科学研究所, 助教 (50418654)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: イヌジステンパーウイルス / リーシュマニア / 二価ワクチン / インターロイキン18 / リバースジェネティクス

Outline of Final Research Achievements

Leishmaniasis is one of the six major tropical diseases documented by WHO. Leishmaniasis is caused by infection with parasite protozoa Leishmania, and dogs harboring Leishmania act as reservoirs. There is presently no vaccine against leishmaniasis.
We generated recombinant canine distemper viruses (rCDVs) that express Leishmania antigens or Th1 cytokine, and evaluated their protective efficacy against Leishmania challenge. As a result, the cocktail of three rCDVs expressing different antigens showed significant vaccine efficiency.

Research Products

• [Journal Article] Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs. (2015)
  • Author(s): Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.
  • Journal Title: PLoS Negl Trop Dis. Volume: 9 Issue: 7 Pages: e0003914-e0003914
  • DOI: 10.1371/journal.pntd.0003914
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Construction of an Expression System for Bioactive IL-18 and Generation of Recombinant Canine Distemper Virus Expressing IL-18 (2014)
  • Author(s): Liu Y, Sato H, Hamana M, Moonan NA, Yoneda M, Xia X, Kai C.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 9 Pages: 1241-1248
  • DOI: 10.1292/jvms.14-0181
  • NAID: 130004693902
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of two recent Japanese field isolates of canine distemper virus and examination of the avirulent strain utility as an attenuated vaccine. (2014)
  • Author(s): Takenaka A, Yoneda M, Seki T, Uema M, Kooriyama T, Nishi T, Fujita K, Miura R, Tsukiyama-Kohara K, Sato H, Kai C.
  • Journal Title: Vet Microbiol Volume: 174(3-4) Issue: 3-4 Pages: 372-81
  • DOI: 10.1016/j.vetmic.2014.10.024
  • Peer Reviewed / Acknowledgement Compliant