Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Outline of Final Research Achievements |
We clarified that Methotrexate (MTX) exposure during gestation day (GD) 11-12 induced hypoplasia of placental basal cell layer in rats. In the present study, the histopathological and molecular biological examinations were performed to clarify the pathogenesis of MTX-induced hypoplasia of placental basal cell layer. The suggestions of the present study were as follows: (1) Apoptosis in hypoplasia of placental basal cell layer was induced by ① both extrinsic and intrinsic pathways, ② oxidative damage of protein and unsaturated fatty acid, and ③ activation of JNK(c-Jun N-terminal kinase)pathway, (2) There were no relation between sensibility to MTX of component cells in placenta and expression levels of dihydrofolate reductase.
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