| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We performed In situ hybridization and immunoelectron analysis on the brain of three dogs in the familial idiopathic epileptic Shetland sheepdogs . Immunohistochemical examination demonstrated a decrease of glutamate transporter (GLT-1) immunolabelings in the cerebral cortex, especially along the sulcus in all familial dogs. In situ hybridization showed GLT-1 mRNA positive reaction in the immunohistochemically decreased area of GLT-1. Immunoelectron examination demonstrated that GLT-1 immunolabelings (immunogold positive) were detected on the endoplasmic reticulum of astrocytes, but few immunolabelings on the plasma membranes of astrocytes in familial dogs, while were clearly detected on the endoplasmic reticulum and plasma membranes of astrocytes in control cases. These results suggest that GLT-1 protein may be normally processed in the endoplasmic reticulum of astrocytes in this canine familial idiopathic epileptic model.
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