Analysis on gene expression profile in oncosheres and early stage metacestodes from Echinococcus multilocularis

• Project/Area Number: 25450425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Tottori University
• Principal Investigator: OKU YUZABURO 鳥取大学, 農学部, 教授 (60133716)
• Co-Investigator(Kenkyū-buntansha): YAGI Kinpei 北海道立衛生研究所, 感染症部, 部長 (70414323)
• Co-Investigator(Renkei-kenkyūsha): KOUGUCHI Hirokazu 北海道立衛生研究所, 感染症部, 研究員 (50435567)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 寄生虫 / 感染防御 / ワクチン開発 / トランスクリプトーム解析 / 次世代シーケンサー / 抗原解析 / 中間宿主 / トランスクリプトーム / エキノコックス / 発育段階 / 診断用抗原 / ワクチン / 虫卵

Outline of Final Research Achievements

Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.

Research Products

• [Journal Article] Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis (2016)
  • Author(s): Fuqiang Huang, Zhisheng Dang, Yutaka Suzuki, Terumi Horiuchi, Kinpei Yagi, Hirokazu Kouguchi, Takao Irie, Kyeongsoon Kim, Yuzaburo Oku
  • Journal Title: PloS Neglected Tropical Diseases Volume: 10 Issue: 4 Pages: 1-22
  • DOI: 10.1371/journal.pntd.0004634
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Expression level analysis of antigen in oncosphere and early stage metecestode from Echinococcus multilocularis (2016)
  • Author(s): Huang fuqiang,八木欣平、孝口裕一、入江隆夫、鈴木穣、堀内映実、金京純、党志勝、奥祐三郎
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2016-03-19
• [Presentation] 次世代シーケンサによる多包条虫の主要抗原の発現ステージのin sillico 解析 (2015)
  • Author(s): 奥祐三郎、黄福強、金京純、八木欣平、孝口裕一、入江隆夫、鈴木穣、堀内映実、党志勝
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 十和田市
  • Year and Date: 2015-09-07