| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The vaccine for neosporosis was developed using alga Chlamydomonas reinhardtii expressed with Neospora caninum surface antigens. C. reinhardtii cells were co-expressed with both Escherichia coli heat-labile enterotoxin B subunit (LT-B) and N. caninum surface antigen, NcSAG1 or NcBAG1. The C. reinhardtii suspensions were inactivated with heating 56℃ for 15 min, and prepared on concentration 5×10＾6/mL as the vaccines. The vaccines were orally inoculated by needle with 0.5 mL/head twice a week. The mice were bled from a caudal vein every week, and antibodies were measured by ELISA. The antibodies were appeared from 3 week after inoculation, and so on. These results suggested that vaccine using alga C. reinhardtii was useful for antibody production by oral.
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