Study of oral vaccine prevent from neosporosis
Project/Area Number |
25450432
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Veterinary medical science
|
Research Institution | Nippon Veterinary and Life Science University |
Principal Investigator |
Ike Kazunori 日本獣医生命科学大学, 獣医学部, 教授 (50159597)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | Neospora caninum / ネオスポラ症 / ワクチン / 経口投与 / アジュバント / 腸管免疫 |
Outline of Final Research Achievements |
The vaccine for neosporosis was developed using alga Chlamydomonas reinhardtii expressed with Neospora caninum surface antigens. C. reinhardtii cells were co-expressed with both Escherichia coli heat-labile enterotoxin B subunit (LT-B) and N. caninum surface antigen, NcSAG1 or NcBAG1. The C. reinhardtii suspensions were inactivated with heating 56℃ for 15 min, and prepared on concentration 5×10^6/mL as the vaccines. The vaccines were orally inoculated by needle with 0.5 mL/head twice a week. The mice were bled from a caudal vein every week, and antibodies were measured by ELISA. The antibodies were appeared from 3 week after inoculation, and so on. These results suggested that vaccine using alga C. reinhardtii was useful for antibody production by oral.
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Report
(4 results)
Research Products
(12 results)
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[Book] 寄生虫病学2014
Author(s)
寄生虫病学共通テキスト編集委員会(池 和憲 他)
Total Pages
222
Publisher
緑書房
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