Pathological study on pathogenicity of emerging arboviruses to cattle

• Project/Area Number: 25450435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Tanaka Shogo 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, ユニット長 (10355216)
• Co-Investigator(Kenkyū-buntansha): 梁瀬 徹 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 上級研究員 (90355214)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Kumiko 国立研究開発法人農研機構, 動物衛生研究部門・病態研究領域・病理ユニット, 上級研究員 (30391444) ; SHIRAFUJI Hiroaki 国立研究開発法人農研機構, 動物衛生研究部門・越境性感染症研究領域・暖地疾病防除ユニット, 主任研究員 (40414726) ; KUBOTA Chikara 鹿児島大学, 共同獣医学部, 教授 (80420652)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アルボウイルス / 感染実験 / 病原性 / ピートンウイルス / 牛胎子 / 牛異常産 / 感染時胎齢 / 髄膜脳脊髄炎 / 牛胎子骨格筋由来筋芽細胞 / 感染親和性 / 牛胎子感染実験 / 牛胎子由来培養筋芽細胞

Outline of Final Research Achievements

To clarify pathogenicity of emerging arboviruses suspected as a cause of cattle congenital abnormalities, cultured myoblast lines derived from an early, middle- and full-term gestational bovine fetus were established, and compared viral tropism on each period by measuring the arbovirus-specific gene amount after inoculation. We also inoculated with Peaton virus (PEAV) in middle-term bovine fetuses and attempted to reproduce the congenital abnormalities, and pathologically examined other bovine fetuses on the 10-day after inoculation. As a result, PEAV showed high proliferative affinity to myoblasts derived from the early fetus, suggesting high sensitivity to early bovine fetuses. In addition, although the congenital anomalies could not reproduce by experimental infection with PEAV in the middle-term bovine fetuses, pathogenicity to cattle was confirmed by the onset of non-suppurative meningoencephalomuyelitis, detection of PEAV genes and antigen localization in the affected fetuses.

Research Products

• [Journal Article] 牛胎子由来培養筋芽細胞におけるオルソブニャウイルスの増殖親和性の相違 (2018)
  • Author(s): 田中省吾、加藤友子、山川 睦、白藤浩明、梁瀬 徹
  • Journal Title: 鹿児島県獣医師会会報 Volume: 61
  • Peer Reviewed
• [Journal Article] Bovine Aroboviruses in Culicoides Biting Midges and Sentinel Cattle in Southern Japan from 2003 to 2013 (2015)
  • Author(s): Kato,T., Shirafuji,H., Tanaka,S., Sato,M., Yamakawa,M., Tsuda,T. and Yanase,T
  • Journal Title: Transbound Emerg Dis Volume: なし Issue: 6 Pages: e160-e172
  • DOI: 10.1111/tbed.12324
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 牛胎子由来培養筋芽細胞における国内新規アルボウイルスの感染動態の比較 (2017)
  • Author(s): 田中省吾、加藤友子、白藤浩明、梁瀬 徹
  • Organizer: 第161回日本獣医学会学術集会