Analyses of energy metabolism adapted to a low oxygen environment in developmental stages of Eimeria

• Project/Area Number: 25450436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University (2015-2016); National Agriculture and Food Research Organization (2013-2014)
• Principal Investigator: Matsubayashi Makoto 大阪府立大学, 生命環境科学研究科, 准教授 (00321076)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: コクシジウム / 呼吸鎖 / 低酸素 / ミトコンドリア / Eimeria tenella / 鶏 / エネルギー代謝 / RNA sequencing / real-time PCR

Outline of Final Research Achievements

Control of chicken coccidia is important issue in poultry industry. The object of this study is focused on producing the safe and new anti-coccidiostat. Consequently, we successfully constructed the methods of purification of Eimeria tenella mitochondria and assay system of　the mitochondria activity. Using the systems, we could found some compounds could inhibit the activities in vitro. Furthermore, these compounds could kill the purified sporozoites and merozoites. These results can show utility for clarifying the respiratory chain activity of Eimeria spp. and finding seed compounds against the parasites.

Research Products

• [Journal Article] Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves (2017)
  • Author(s): Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Acta Parasitologica Volume: 62(1) Issue: 1 Pages: 214-220
  • DOI: 10.1515/ap-2017-0026
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody (2016)
  • Author(s): Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Hyun S. Lillehoj, Haruo Matsuda, Shigeo Takenaka, Takeshi Hatta, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Parasitology Research Volume: 115(11) Pages: 4123-4128
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transcriptional profiles of virulent and precocious strains of Eimeria tenella at sporozoite stage; novel biological insight into attenuated asexual development (2016)
  • Author(s): Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Junya Yamagishi, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji
  • Journal Title: Infection, Genetics and Evolution Volume: 40 Pages: 54-62
  • DOI: 10.1016/j.meegid.2016.02.021
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] First report of molecular identification of Cystoisospora suis in a piglet with lethal diarrhea in Japan (2016)
  • Author(s): Makoto Matsubayashi, Hideko Takayama, Masahiro Kusumoto, Misato Murata, Yuka Uchiyama, Masaya Kaji, Kazumi Sasai, Ryosaku Yamaguchi, Tomoyuki Shibahara
  • Journal Title: Acta Parasitologica Volume: 61(2) Issue: 2 Pages: 406-11
  • DOI: 10.1515/ap-2016-0054
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice (2016)
  • Author(s): Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo
  • Journal Title: Acta Parasitologica Volume: 115(1) Issue: 1 Pages: 211-5
  • DOI: 10.1007/s00436-015-4737-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A serological survey of antobodied against Leptospira spp. from wild raccoons (Procyon lotor) in Osaka, Japan (2016)
  • Author(s): Saeki Jun, Nakanishi Hitoshi, Masubuchi Katsuo, Matsubayashi Makoto, Furuya Masaru, Tani Hiroyuki, Sasai Kazumi
  • Journal Title: Asian Journal of Animal and Veterinary Advances Volume: 未定
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic identification of Entamoeba polecki subtype 3 from pigs in Japan and characterisation of its pathogenic role in ulcerative colitis (2015)
  • Author(s): Makoto Matsubayashi, Naoko Murakoshi, Tetsuya Komatsu, Masaharu Tokoro, Makoto Haritani, Tomoyuki Shibahara
  • Journal Title: Infection, Genetics and Evolution Volume: 36 Pages: 8-14
  • DOI: 10.1016/j.meegid.2015.08.030
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localization of heat shock protein 110 in canine mammary gland tumor (2015)
  • Author(s): Satoru Okada, Masaru Furuya, Shigeo Takenaka, Ayano Fukui, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 167(3-4) Issue: 3-4 Pages: 139-46
  • DOI: 10.1016/j.vetimm.2015.07.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Onchocerca takaokai n. sp. (Nematoda: Filarioidea) in Japanese wild boars (Sus scrofa leucomystax): Description and molecular identification of intradermal females (2015)
  • Author(s): Shigehiko Uni, Masako Fukuda, Takeshi Agatsuma, Odile Bain, Yasushi Otsuka, Jun Nakatani, Makoto Matsubayashi, Masashi Harada, Hasmahzaiti Omar, Rosli Ramli, Rosli Hashim, Mohd Sofian Azirun, Hiroyuki Takaoka
  • Journal Title: Parasitology International Volume: 64 Issue: 6 Pages: 493-502
  • DOI: 10.1016/j.parint.2015.07.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] First molecular identification of Entamoeba polecki in a piglet in Japan and implications for aggravation of ileitis by coinfection with Lawsonia intracellularis (2015)
  • Author(s): Makoto Matsubayashi, Kenta Kanamori, Masayuki Sadahiro, Masaharu Tokoro, Niichiro Abe, Makoto Haritani, Tomoyuki Shibahara
  • Journal Title: Parasitology Research Volume: 114(8) Issue: 8 Pages: 3069-73
  • DOI: 10.1007/s00436-015-4509-9
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The detection of toxigenic Corynebacterium ulcerans from cats with nasal inflammation in Japan. (2015)
  • Author(s): Saeki J, Katsukawa C, Matsubayashi M, Nakanishi H, Furuya M, Tani H, Sasai K.
  • Journal Title: Epidemiol Infect. Volume: 1 Issue: 12 Pages: 1-6
  • DOI: 10.1017/s0950268814003550
  • Peer Reviewed / Open Access
• [Journal Article] 豚の消化器疾患におけるEntamoeba poleckiとEntamoeba suisの病原性 (2015)
  • Author(s): 金森健太, 貞弘真行, 松林 誠, 所 正治, 阿部 仁一郎, 播谷 亮, 芝原友幸
  • Journal Title: 日本豚病研究会報 Volume: 67 Pages: 28-33
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 離乳後問題となる疾病 (2015)
  • Author(s): 芝原友幸, 宮﨑綾子, 生澤充隆, 山口遼作, 松林 誠
  • Journal Title: 日本豚病研究会報 Volume: 66 Pages: 22-25
  • NAID: 50009481708
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] パラフィン包埋切片を用いた種特異的PCRによる子牛の出血性直腸炎におけるコクシジウムの遺伝子学的同定 (2015)
  • Author(s): 是枝輝紀，野尻麻里子，堂下さつき，松林 誠，芝原友幸，岡野良一
  • Journal Title: 日本獣医師会雑誌 Volume: 68 Pages: 297-300
  • NAID: 130005078180
  • Peer Reviewed
• [Journal Article] 牛のクリプトスポリジウム下痢症 「牛のクリプトスポリジウム症」 (2015)
  • Author(s): 松林 誠
  • Journal Title: 臨床獣医 Volume: 33(3) Pages: 12-15
• [Journal Article] 豚のアメーバ症；出血性大腸炎の豚から同定されたEntamoeba suis (2015)
  • Author(s): 鈴田史子、松林 誠、寺山好美、下條憲吾、油井 武、播谷 亮、芝原友幸
  • Journal Title: 日本豚病研究会報 Volume: 65 Pages: 44-50
  • NAID: 50009252442
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents (2015)
  • Author(s): Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Masashi Tsujio, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Kozo Fujisaki, Toshihiro Matsui and Tomohide Matsuo
  • Journal Title: Acta Parasitologica Volume: 60(2) Pages: 190-195
  • Peer Reviewed / Open Access
• [Journal Article] DB-AT: a 2015 update to the Full-parasites database brings a multitude of new transcriptomic data for apicomplexan parasites (2014)
  • Author(s): Jakalski, M, Wakaguri H, Kischka T, Nishikawa Y, Kawazu S, Matsubayashi M, Kawahara F, Tsuji N, Cao S, Sunaga F, Xuan X, Okubo K, Igarashi I, Tuda J, Mongan A, Eshita Y, Maeda R, Makaｌowski W, Suzuki Y, Yamagishi J
  • Journal Title: Nucleic Acids Research Volume: 43 Issue: D1 Pages: D631-D636
  • DOI: 10.1093/nar/gku1240
  • NAID: 120005602520
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Unusual ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity (2014)
  • Author(s): Makoto Matsubayashi, Fumiko Suzuta, Yoshimi Terayama, Kengo Shimojo, Takeshi Yui, Makoto Haritani, Tomoyuki Shibahara
  • Journal Title: Parasitology Research Volume: 113(8) Issue: 8 Pages: 3023-8
  • DOI: 10.1007/s00436-014-3965-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic analysis of Enterobius vermicularis isolated from a chimpanzee with lethal hemorrhagic colitis and pathology of the associated lesions (2014)
  • Author(s): Yuji Yaguchi, Sachi Okabayashi, Niichiro Abe, Haruhisa Masatou, Shinya Iida, Isao Teramoto, Makoto Matsubayashi, Tomoyuki Shibahara
  • Journal Title: Parasitology Research Volume: 113(11) Issue: 11 Pages: 4105-9
  • DOI: 10.1007/s00436-014-4080-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre-and post-weaned piglets and adult pigs in Japan. (2014)
  • Author(s): Takeshi Yui, Toshiyuki Nakajima, Norishige Yamamoto, Marina Kon, Niichiro Abe, Makoto Matsubayashi, Tomoyuki Shibahara.
  • Journal Title: Parasitology Research Volume: 113(1) Issue: 1 Pages: 359-65
  • DOI: 10.1007/s00436-013-3662-2
  • Peer Reviewed
• [Journal Article] Survey on gastrointestinal parasites and detection of Cryptosporidium spp. on cattle in West Java, Indonesia (2014)
  • Author(s): Sylvia Maharani Ananta, Suharno, Adi Hidayat, Makoto Matsubayashi.
  • Journal Title: Asian Pacific Journal of Tropical Medicine. Volume: 7(3) Issue: 3 Pages: 197-201
  • DOI: 10.1016/s1995-7645(14)60020-1
  • Peer Reviewed
• [Journal Article] Localization of Eimeripain, an <i>Eimeria tenella</i> Cathepsin B-Like Cysteine Protease, during Asexual and Sexual Intracellular Development in Chicken Ceca (2014)
  • Author(s): Makoto Matsubayashi, Takeshi Hatta, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Kayoko Yamaji, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 4 Pages: 531-537
  • DOI: 10.1292/jvms.13-0509
  • NAID: 130003382415
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Elongation factor-1α is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum. (2013)
  • Author(s): Matsubayashi M, Teramoto-Kimata I, Uni S, Lillehoj HS, Matsuda H, Furuya M, Tani H, Sasai K.
  • Journal Title: J Biol Chem. Volume: 288(47) Issue: 47 Pages: 34111-34120
  • DOI: 10.1074/jbc.m113.515544
  • Peer Reviewed
• [Journal Article] High-throughput RNA sequencing profiles and transcriptional evidence of aerobic respiratory enzymes in sporulating oocysts and sporozoites of Eimeria tenella. (2013)
  • Author(s): Makoto Matsubayashi, Takeshi Hatta, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji.
  • Journal Title: Infection, Genetics and Evolution Volume: 18 Pages: 269-76
  • DOI: 10.1016/j.meegid.2013.06.002
  • Peer Reviewed
• [Presentation] Cryptosporidium parvum における呼吸鎖関連酵素の特性解析 (2017)
  • Author(s): 髙橋 和瑛、松林 誠、稲岡 ダニエル 健、八田 岳士、寺本 勲、 金子 明、所 正治、古家 優、谷 浩行、辻 尚利、笹井 和美、北 潔
  • Organizer: 第86回日本寄生虫学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2017-05-28
• [Presentation] Cryptosporidium parvum における呼吸鎖関連酵素の特性解析 (2016)
  • Author(s): 高橋 和瑛、松林 誠、稲岡 ダニエル 健、八田 岳士、寺本 勲、金子 明、所 正治、古家 優、谷 浩行、辻 尚利、笹井 和美、北 潔.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Presentation] 幼雛におけるEimeria tenella弱毒株の作出および病理組織学的解析 (2016)
  • Author(s): 松林 誠、川原 史也、八田 岳士、山岸 潤也、磯部 尚、寺本 勲、金子 明、笹井 和美、北 潔、辻 尚利
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ(宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 豚の大腸から見出されたEntamoeba属原虫の種および遺伝子型の同定と病理組織学的解析 (2016)
  • Author(s): 芝原 友幸、鈴田 史子、金森 健太、村越 奈穂子、小松 徹也、松林 誠、阿部 仁一郎、播谷 亮、所 正治
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ(宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 抗鶏コクシジウム症薬の開発を目指したEimeria tenella Dihydroorotate dehydrogenaseの発現・精製と結晶化 (2015)
  • Author(s): 長濱 まどか、佐藤 暖、志波 智生、松林 誠、 稲岡 ダニエル健、辻 尚利、北 潔、原田 繁春
  • Organizer: 第88回日本生化学会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2015-12-01
• [Presentation] Eimeria tenella のミトコンドリア呼吸鎖酵素活性の測定と阻害剤による虫体殺滅効果 (2015)
  • Author(s): 松林 誠、稲岡 ダニエル 健、小松谷 啓介、八田 岳士、三好 猛晴、磯部 尚、川原 史也、山岸 潤也、彦坂 健児、佐藤 暖、志波 智生、原田 繁春、北 潔、辻 尚利
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北里大学(十和田）
  • Year and Date: 2015-09-07
• [Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella (2014)
  • Author(s): Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Takeharu Miyoshi, Junya Yamagishi, Anisuzzaman, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Akira Iwata, Naotoshi Tsuji
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Disease
  • Place of Presentation: Dresden University of Technology (Dresden, Germany)
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella (2014)
  • Author(s): Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Takeharu Miyoshi, Junya Yamagishi, Anisuzzaman, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Akira Iwata, Naotoshi Tsuji
  • Organizer: 11th International Coccidiosis Conference
  • Place of Presentation: Dresden University of Technology (Dresden, Germany)
  • Year and Date: 2014-09-26 – 2014-09-30
• [Presentation] Eimeria tenella スポロゾイトのミトコンドリア精製法の確立と呼吸鎖酵素活性 (2014)
  • Author(s): 松林 誠、稲岡 ダニエル 健、小松谷 啓介、八田 岳士、三好 猛晴、磯部 尚、川原 史也、山岸 潤也、彦坂 健児、佐藤 暖、志波 智生、原田 繁春、北 潔、辻 尚利
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(北海道札幌市)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察 (2014)
  • Author(s): 小野 結菜、松林 誠、川口 博明、辻尾 祐志、田仲 哲也、三好 宣彰、松井 利博、松尾 智英
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(北海道札幌市)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 次世代シークエンサーを用いたEimeria tenella 強毒株と弱毒株スポロゾイトの遺伝子発現プロファイリング (2014)
  • Author(s): 松林 誠、川原 史也、八田 岳士、三好 猛晴、山岸 潤也、Anisuzzaman M、磯部 尚、北 潔、岩田 晃、辻 尚利.
  • Organizer: 第83回日本寄生虫学会大会学術集会
  • Place of Presentation: 愛媛大学
• [Presentation] Eimeria tenella 強毒株と弱毒株スポロゾイトの比較トランスクリプトーム解析. 、238. (2013)
  • Author(s): 松林 誠、川原 史也、八田 岳士、三好 猛晴、山岸 潤也、M アニスザマン、志村 亀夫、磯部 尚、岩田 晃、辻 尚利.
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] Eimeria tenella 強毒株と弱毒株スポロゾイトの網羅的遺伝子発現比較解析 (2013)
  • Author(s): 松林 誠、川原史也、八田岳士、三好猛晴、山岸潤也、アニスザマン、志村亀夫、磯部 尚、北潔、岩田 晃、辻 尚利.
  • Organizer: 第11回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 長崎大学