| Project/Area Number |
25450439
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
KAKU Yoshihiro 国立感染症研究所, 獣医科学部, 主任研究官 (70392321)
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| Research Collaborator |
KAWAHARA Masahiro 東京大学, 大学院工学系研究科, 准教授
ASAI Tomohiro 静岡県立大学, 薬学部, 准教授
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 狂犬病 / intrabody / 治療 / ドラッグデリバリー / ナノ粒子 / 狂犬病ウイルス / scFv / 遺伝子治療 / 血液脳関門 / 人獣共通感染症 |
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| Outline of Final Research Achievements |
Rabies virus (RABV) travels via retrograde fast axonal transport within axons in peripheral nerves to central nervous system. After clinical symptoms appear, the mortality rate is almost 100% and no reliable treatment is available. In this study, aiming to develop the treatment of rabies after the onset, several single chain variable fragments (scFvs) against RABV were produced, and some of which were confirmed to bind to RABV protein inside the cells and inhibit viral propagation. Also, nanoparticle-based in vitro delivery system was constructed to deliver scFv-expressing plasmid DNAs to neural cells.
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