| Project/Area Number |
25450441
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Nagano Masashi 北海道大学, (連合)獣医学研究科, 准教授 (70312402)
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| Research Collaborator |
HUANG Weiping
KANG Sung-Sik
KOYAMA Keisuke
NAGAI Katsuhisa
SAKAGUCHI Kenichiro
YANG Yinghua
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 牛 / 卵子 / 体外発育 / 体外受精 / 卵胞 / 体外発育培養 / 野生動物 / 精子 / IVG / IVM / 初期胞状卵胞 / 受精卵移植 |
|---|
| Outline of Final Research Achievements |
I tried to improve the culture system of oocyte-granulosa complexes (OGCs) derived from bovine early antral follicles. As the results, I produced offspring effectively by my developed system, and it was the third case in the world. The result indicates the embryos derived from the culture system have normality the same as in vivo derived embryos. When I added born morphogenetic protein-4 (BMP-4) to culture medium, the mean diameter of granulosa cells became smaller and progesterone secretion from granulosa cells was inhibited. These mean that BMP-4 inhibits the luteinization of granulosa cells. In addition, I compared the developmental competence of OGCs derived from ovaries having different antral follicle count (AFC). OGCs derived from high and low AFCs showed similar viability during growth culture; however, the mean number of granulosa cells was higher in high AFC group. Oocytes derived from high AFC have higher activity of mitochondria and showed higher nuclear maturation rate.
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