| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In order to develop oral inactivated multivalent Salmonella vaccine, the fusion proteins of cholera toxin B subunit (CTB) or heat-labile enterotoxin B subunit (LTB) of ETEC, which are the suitable candidates for mucosal adjuvant, and fliC as Salmonella Enteritidis (SE) vaccine candidate protein were expressed on the surface of E. coli by using E. coli surface display system. The chickens were immunized by oral, intra-muscular or intra-ocular route with the inactivated E. coli expressed fusion proteins. SE flagellar specific serum IgG titer and intestinal mucous IgA concentration were increased after immunization. The immunized chickens with intra-ocular administration of inactivated E. coli expressed LTB and fliC fusion protein showed significantly decreased bacterial counts in the cecal contents. Our findings obtained in this study can be applied to development of simple and safety mucosal Salmonella vaccine.
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