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Development of oral inactivated multivalent Salmonella vaccine using E. coli cell surface display system

Research Project

Project/Area Number 25450449
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionOsaka Prefecture University

Principal Investigator

Tani Hiroyuki  大阪府立大学, 生命環境科学研究科, 准教授 (00305658)

Co-Investigator(Kenkyū-buntansha) 笹井 和美  大阪府立大学, 生命環境科学研究科, 教授 (70211935)
古家 優  大阪府立大学, 生命環境科学研究科, 助教 (30500706)
松林 誠  大阪府立大学, 生命環境科学研究科, 准教授 (00321076)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsサルモネラ / 不活化ワクチン / 大腸菌表層ディスプレイ / 粘膜ワクチン / 経口ワクチン
Outline of Final Research Achievements

In order to develop oral inactivated multivalent Salmonella vaccine, the fusion proteins of cholera toxin B subunit (CTB) or heat-labile enterotoxin B subunit (LTB) of ETEC, which are the suitable candidates for mucosal adjuvant, and fliC as Salmonella Enteritidis (SE) vaccine candidate protein were expressed on the surface of E. coli by using E. coli surface display system. The chickens were immunized by oral, intra-muscular or intra-ocular route with the inactivated E. coli expressed fusion proteins. SE flagellar specific serum IgG titer and intestinal mucous IgA concentration were increased after immunization. The immunized chickens with intra-ocular administration of inactivated E. coli expressed LTB and fliC fusion protein showed significantly decreased bacterial counts in the cecal contents. Our findings obtained in this study can be applied to development of simple and safety mucosal Salmonella vaccine.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results)

  • [Presentation] 鶏サルモネラ症防除を目指した大腸菌を用いた組換え不活化経口ワクチンの開発2014

    • Author(s)
      稲垣果歩、谷 浩行、小西 翔、松林 誠、古家 優、笹井和美
    • Organizer
      第157回 日本獣医学会学術集会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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