Lard-based high-fat diet worsens liver injury but attenuates lung injury in endotoxemic rats

• Project/Area Number: 25450450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kobe University
• Principal Investigator: Miyoshi Makoto 神戸大学, 保健学研究科, 助教 (50433389)
• Co-Investigator(Kenkyū-buntansha): Usami Makoto 神戸大学, 保健学研究科, 教授 (00193855)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 高脂肪食 / 敗血症 / トリブチリン

Outline of Final Research Achievements

Lard-based high-fat diet (HFD) feeding rats in endotoxemia worsened liver injury, but attenuated lung injury. HFD feeding enhanced proinflammatory lipid mediators 1.5 h after lipopolysaccharide injection suggesting relation to liver injury. The elevation of pulmonary secretory leukocyte protease inhibitor, anti-inflammatory protein, might be associated with the reduction of lung injury in these rats.
Tributyrin, prodrug of butyrate, suppressed endotoxin-induced lipid metabolism disorder. Oral tributyrin administration prevents elevation plasma triglyceride, total cholesterol and low-density lipoprotein cholesterol levels through improved fatty acid oxidation, PPARs and histone H3 in the liver in endotoxemic rats.

Research Products

• [Journal Article] Oral tributyrin prevents endotoxin-induced lipid metabolism disorder (2015)
  • Author(s): M. Miyoshi, N. Iizuka, S. Sakai, M. Fujiwara, M. Aoyama-Ishikawa, N. Maeshige, Y. Hamada, M. Takahashi, M. Usami
  • Journal Title: Clinical Nutrition ESPEN Volume: 10 Issue: 2 Pages: e83-e88
  • DOI: 10.1016/j.clnesp.2015.02.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lard-based high-fat diet increases secretory leukocyte protease inhibitor expression and attenuates the inflammatory response of acute lung injury in endotoxemic rats (2015)
  • Author(s): M. Fujiwara, M. Miyoshi, S. Sakai, A. Nishiokada, M. Aoyama-Ishikawa, N. Maeshige, Y. Usami, Y. Hamada, M. Takahashi, M. Usami
  • Journal Title: Clinical Nutrition Volume: 34 Issue: 5 Pages: 997-1009
  • DOI: 10.1016/j.clnu.2014.11.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Changes of hepatic lipid mediators associated with intake of high-fat diet for 12 weeks in endotoxemic rats using LC-ESI-MS/MS (2015)
  • Author(s): A. Nishiokada, M. Miyoshi, M. Fujiwara, M. Aoyama-Ishikawa, N. Maeshige,M. Takahashi, Y. Hamada, Y. Usami, M. Honda, M. Arita, M. Usami
  • Journal Title: Clinical Nutrition Volume: 34 Issue: 4 Pages: 685-693
  • DOI: 10.1016/j.clnu.2014.07.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mild obesity reduces survival and adiponectin sensitivity in endotoxemic rats (2013)
  • Author(s): S. Sakai, N. Iizuka, M. Fujiwara, M. Miyoshi, M. Aoyama, N. Maeshige, Y. Hamada, Y. Usami, M. Usami.
  • Journal Title: Journal of surgical research Volume: 185 Pages: 353-363
  • NAID: 120005457049
  • Peer Reviewed
• [Presentation] 12週間ラード含有高脂肪食摂食が急性炎症下の肝組織中PPARαとPPARγ発現を上昇させる (2015)
  • Author(s): 甲斐元規、三好真琴、西山雄也、梶田歩、石川（青山）倫子、前重伯壮、井上岳人、植村弥希子、山下勇人、古賀由華、宇佐美眞
  • Organizer: 第6回機能油脂懇話会
  • Place of Presentation: 明治大学駿河台キャンパス（東京都・千代田区）
  • Year and Date: 2015-11-14
• [Presentation] Up-regulation of hepatic PPARα and PPARγ in endotoxemic rats by feeding lard-rich high-fat diet for 12 weeks (2015)
  • Author(s): M. Kai, M. Fujiwara, M. Miyoshi, Y. Nishiyama, M. Aoyama-Ishikawa, N. Maeshige, T. Inoue, M. Uemura, H. Yamashita, Y. Koga, M. Usami
  • Organizer: The 37th Congress of the European Society of Parenteral and Enteral Nutrition
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2015-09-05
• [Presentation] 4 週間あるいは 12 週間ラード含有高脂肪食摂食による急性炎症下の肝組織中脂肪酸分画，脂肪酸酸化・合成酵素の変動 (2014)
  • Author(s): 甲斐元規、藤原麻有、三好真琴、西山雄也、青山倫子、前重伯壮、井上岳人、植村弥希子、山下勇人、高橋路子、濱田康弘、宇佐美眞
  • Organizer: 第12回機能性食品医用学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-12-13 – 2014-12-14
• [Presentation] Changes of hepatic lipid mediators associated with intake of high-fat diet for twelve weeks in endotoxemic rats using LC-ESI-MS/MS (2014)
  • Author(s): A. Nishiokada, M. Miyoshi, M. Fujiwara, M. Aoyama-Ishikawa, Y. Nishiyama, M. Kai, N. Maeshige, M. Takahashi, Y. Hamada, Y. Usami, M. Arita, M. Usami
  • Organizer: The 36th Congress of the European Society of Parenteral and Enteral Nutrition.
  • Place of Presentation: The modern Geneva Convention Centre (Geneva, Switzerland)
  • Year and Date: 2014-09-06 – 2014-09-09
• [Presentation] ラード含有高脂肪食投与ラットにおけるエンドトキシン血症下の肝組織中脂質メディエーター変動 -LC-ESI-MS/MS解析- (2014)
  • Author(s): 西山雄也、西岡田亜矢、藤原麻有、三好真琴、甲斐元規、青山倫子、山下勇人、前重伯壮、高橋路子、濱田康弘、有田誠、宇佐美眞
  • Organizer: 第51回日本外科代謝栄養学会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] ラード含有高脂肪食投与によるラット肝障害下の脂肪酸分画の変動 (2014)
  • Author(s): 甲斐元規、藤原麻有、西岡田亜矢、三好真琴、西山雄也、青山倫子、前重伯壮、山下勇人、井上岳人、高橋路子、濱田康弘、宇佐美眞
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学（北海道札幌市）
  • Year and Date: 2014-05-31 – 2014-06-01
• [Presentation] 12週間の高脂肪食投与によるラットエンドトキシン血症下の肝組織中脂質メディエーターと脂肪酸代謝酵素の経時変化 (2014)
  • Author(s): 西山雄也、西岡田亜矢、藤原麻有、三好真琴、甲斐元規、青山倫子、山下勇人、井上岳人、前重伯壮、高橋路子、濱田康弘、有田誠、宇佐美眞
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学（北海道札幌市）
  • Year and Date: 2014-05-31 – 2014-06-01