| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Prototheca zopfii is an achlorophyllic alga that causes bovine mastitis, resulting in a reduction in milk production and the secretion of thin, watery milk with white flakes. This study evaluated the use of an ELISA system for distinguishing cows with mastitis due to P. zopfii genotype 2 from cows with chronic candidal mastitis and healthy cows. Moreover, we investigated the transitional changes of specific antibody titer of healthy cows injected with inactivated P. zopfii genotype-2 cells. This ELISA system exhibited the highest sensitivity (94%) and specificity (100%) for the chronic protothecal mastitis cows when the positive cutoff value was set at 43.4 EU. Anti-protothecal IgG titers were positive in all cows after inoculation with inactivated P. zopfii genotype 2 cells. These results indicated that ELISA detection of anti-prototheca IgG in serum provided specificity and sensitivity sufficient for use in diagnosing protothecal mastitis.
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