| Project/Area Number |
25450454
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Veterinary medical science
|
|---|
| Research Institution | Nippon Veterinary and Life Science University |
|---|
Principal Investigator |
OMI TOSHINORI 日本獣医生命科学大学, 獣医学部, 教授 (40296091)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TUCHIDA Shuichi 日本獣医生命科学大学, 獣医学部, 教授 (20217326)
BONKOBARA Makoto 日本獣医生命科学大学, 獣医学部, 教授 (50343611)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 輸血医療 / 遺伝子検査 / 血液型物質 / 小動物 / 輸血 / 血液型 / イヌＤＥＡ / ネコＡＢ / 抗原エピトープ / 遺伝子 / イヌDEA1.1 / ネコAB / 輸血副作用 / ゲノムワイド関連解析 / 責任候補遺伝子 / 遺伝子発現解析 / イヌDEA式血液型 / ネコＡＢ式血液型 |
|---|
| Outline of Final Research Achievements |
The dog erythrocyte antigen (DEA) and cat AB blood group system are important in transfusion-related research. Here we investigated the molecular basis of dog and cat erythrocyte blood group antigens. Of 282 dogs, 71.6% were positive and 28.4% were negative for DEA 1.1. Of 772 cats, 95.1%, 4.9%, and 0% of cats had the A, B, and AB blood group, respectively. To identify a candidate molecule expressed on the dog erythrocyte membrane associated with dog blood group, we performed the agglutination test using a lectin (anti-NeuGC). Dog RBCs were then divided into two types: positive and negative cells. Results suggested the CMAH gene associated with the NeuGC expression may be associated with the DEA system. In cat,the CMAH gene encodes the antigens in the cat AB blood group system. In the gene, we identified eight SNPs in blood type B cats, including four novel SNPs. These data provide information for developing a DNA-testing method to identify blood groups.
|
