Development of diagnostic examination for cat obesity

• Project/Area Number: 25450456
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Yamamoto Ichiro 日本獣医生命科学大学, 獣医学部, 准教授 (00424763)
• Co-Investigator(Renkei-kenkyūsha): NAKAO Nobuhiro 日本獣医生命科学大学, 応用生命科学部, 准教授 (60377794)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ネコ / GPR120 / GPR40 / 肥満

Outline of Final Research Achievements

Obesity due to lifestyle changes in domestic animals has increased noticeably in the cat is known to significantly increase the risk of type 2 diabetes. It is definite advantage to use of genetic or biochemical examination for preventing obese in cats. In this research project, we challenged to develop a new diagnostic examination of free-fatty acid receptor 4 (FFAR4 / GPR120) gene for preventing cat obesity.

Research Products

• [Journal Article] Genetic variants of the unsaturated fatty acid receptor GPR120 relating to obesity in dogs (2015)
  • Author(s): Miyabe M, Gin A, Onozawa E, Daimon M, Yamada H, Oda H, Mori A, Momota Y, Azakami D, Yamamoto I, Mochizuki M, Sako T, Tamura K, Ishioka K
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 10 Pages: 1201-1206
  • DOI: 10.1292/jvms.15-0031
  • NAID: 130005107589
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of free fatty acid receptors GPR40/FFAR1 and GPR120/FFAR4 in a domestic cat. (2015)
  • Author(s): Habara M, Tamanuki M, Ishikawa S, Takemitsu H, Mori N, Okada Y, Nakao N, Kawasumi K, Ishioka K, Arai T.
  • Journal Title: Asian Journal of Animal and Veterinary Advances Volume: 10 Issue: 4 Pages: 185190-185190
  • DOI: 10.3923/ajava.2015.185.190
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sirtuin 1 suppresses nuclear factor κB induced transactivation and pro-inflammatory cytokine expression in cat fibroblast cells (2015)
  • Author(s): Ishikawa, Takemitsu H, Habara M, Mori N, Yamamoto I, Arai T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 12 Pages: 1681-1684
  • DOI: 10.1292/jvms.15-0245
  • NAID: 130005115338
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Changes in fatty acid composition in tissue and serum of obese cats fed a high fat diet. (2015)
  • Author(s): Fujiwara M, Mori N, Sato T, Tazaki H, Ishikawa S, Yamamoto I, Arai T.
  • Journal Title: BMC Veterinary Research Volume: 11 Issue: 1 Pages: 200-200
  • DOI: 10.1186/s12917-015-0519-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Preliminary analysis of modified low density lipoproteins in the serum of healthy and obese dogs and cats. (2015)
  • Author(s): Mori N, Okada Y, Tsuchida N, Hatano Y, Habara M, Ishikawa S, Yamamoto I, Arai T.
  • Journal Title: Frontiers in veterinary Science Volume: 17 Pages: 34-34
  • DOI: 10.3389/fvets.2015.00034
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Age effects on plasma cholesterol and triglyceride profiles and metabolite concentrations in dogs. (2014)
  • Author(s): Kawasumi K, Kashiwado N, Okada Y, Sawamura M, Sasaki Y, Iwazaki E, Mori N, Yamamoto I, Arai T.
  • Journal Title: BMC Vet Res Volume: 10 Issue: 1 Pages: 57-57
  • DOI: 10.1186/1746-6148-10-57
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of plasma lipoprotein profiles and malondialdehyde between hyperlipidemia dogs with/without treatment. (2014)
  • Author(s): Li G, Kawasumi K, Okada Y, Ishikawa S, Yamamoto I, Arai T, Mori N.
  • Journal Title: BMC Vet Res Volume: 10 Issue: 1 Pages: 67-67
  • DOI: 10.1186/1746-6148-10-67
  • Peer Reviewed / Open Access
• [Journal Article] Effects of administration of Licorice flavonoid supplement on lipid metabolism in obese dogs. (2014)
  • Author(s): Kawasumi K, Okada Y, Kashiwado N, Iwasaki E, Mori N, Yamamoto I, Arai T.
  • Journal Title: Asian Journal of Animal and Veterinary Advances. Volume: 9 Pages: 721-721
  • Peer Reviewed
• [Journal Article] Changes in plasma fatty acid composition in hyperlipidemia dogs. (2013)
  • Author(s): Fujiwara M, Sato T, Tazaki H, Yamamoto I, Kawasumi K, Arai T.
  • Journal Title: Asian J. Anim. Vet. Adv. Volume: 1 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Pilot Study to Determine Peripheral Blood Leukocyte mRNA Expression Profile of Energy Homeostasis Genes to Identify Potential Predictive Biomarkers of Obesity in Dogs (2013)
  • Author(s): Li G, Lee P, Yamamoto I, Mori N, Arai T.
  • Journal Title: J Anim Vet Advance Volume: 12 Pages: 69-75
  • Peer Reviewed
• [Journal Article] Changes in expression levels of neurotensin precursor and receptor mRNA in chicken intestinal tissues and liver during late embryonic and posthatching development (2013)
  • Author(s): Minoru Tanaka, Nobuhiro Nakao, Ichiro Yamamoto, Nobumiti Tsushima, Yoshiyuki Ohta
  • Journal Title: Poultry Science Volume: 92 Issue: 10 Pages: 2765-2771
  • DOI: 10.3382/ps.2012-02939
  • Peer Reviewed
• [Journal Article] Mechanism of insulin production in canine bone marrow derived mesenchymal stem cells. (2013)
  • Author(s): Takemitsu H, Zhao D, Ishikawa S, Michishita M, Arai T, Yamamoto I.
  • Journal Title: Gen Comp Endocrinol. Volume: 189 Pages: 1-6
  • DOI: 10.1016/j.ygcen.2013.04.009
  • Peer Reviewed
• [Journal Article] Change in mRNA expression of sirtuin 1 and sirtuin 3 in cats fed on high fat diet. (2013)
  • Author(s): Ishikawa S, Li G, Takemitsu H, Fujiwara M, Mori N, Yamamoto I, Arai T.
  • Journal Title: BMC Vet Res. Volume: 9 Pages: 187-187
  • Peer Reviewed
• [Journal Article] A comparison of metabolic parameters between obese and non-obese healty domestic dogs in japan. (2013)
  • Author(s): Mori N, Takemitsu H, Okada Y, Yamamoto I, Arai T.
  • Journal Title: Asian J. Anim. Vet. Adv. Volume: 8 Issue: 7 Pages: 863-873
  • DOI: 10.3923/ajava.2013.863.873
  • Peer Reviewed
• [Presentation] イヌおよびネコNF-κBp65サブユニットの機能解析とSirtuin1の影響 (2014)
  • Author(s): 石川真悟、羽原誠、武光浩史、森伸子、山本一郎、新井敏郎
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] イヌとネコにおける脂肪酸受容体GPR120のcDNAクローニングおよび各組織におけるmRNA発現量の解析 (2014)
  • Author(s): 羽原誠、石川真悟、李格賓、武光浩史、岡田ゆう紀、森伸子、川角浩、山本一郎、新井敏郎
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] cDNA CLONING AND mRNA EXPRESSION OF CAT GPR40. (2014)
  • Author(s): Ichiro Yamamoto, Makoto Habara, Shingo Ishikawa, Gebin Li, Hiroshi Takemitsu, Yuki Okada, Nobuko Mori, Nobuhiro Nakao, Koh Kawasumi, Toshiro Arai.
  • Organizer: 16th International Society for Animal Clinical Pathology
  • Place of Presentation: コペンハーゲン大学
  • Year and Date: 2014-06-25 – 2014-06-29
• [Presentation] 猫の肥満発症メカニズムとその対策 (2014)
  • Author(s): 新井敏郎、森伸子、川角浩、山本一郎
  • Organizer: 日本獣医内科学アカデミー第10回記念学術集会
  • Place of Presentation: 横浜
• [Presentation] 高脂肪食給与ネコにおけるSirtuin1, 3遺伝子発現量の変動 (2013)
  • Author(s): 石川真悟、李格賓、武光浩史、藤原めぐみ、森伸子、山本一郎、新井敏郎
  • Organizer: 第156回日本獣医学会
  • Place of Presentation: 岐阜