Analysis of the function of the special equilibrium organ of the bird

• Project/Area Number: 25450463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tottori University
• Principal Investigator: Kitamura Naoki 鳥取大学, 農学部, 准教授 (80301951)
• Co-Investigator(Kenkyū-buntansha): SHIBUYA Izumi 鳥取大学, 農学部, 教授 (50162649)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経生理学 / 鳥類 / 平衡感覚 / イオンチャネル / 受容体 / パッチクランプ / 細胞内カルシウム / アクセサリーローブ / アセチルコリン / ムスカリン型受容体 / 細胞内カルシウム濃度変動 / 電位依存性カルシウムチャネル / 電気生理学 / アトロピン / N型電位依存性Ca2+チャネル / CaV2.2 / ω-conotoxin GVIA

Outline of Final Research Achievements

Electrical activity and changes in the intracellular Ca2+ concentration were measured from spinal accessory lobe (AL) neurons of the chick.
AL neurons showed no significant response to the stimulation with the hypertonic solution that caused increase in the cell volume and stretch of the cell membrane. The stimulation with acetylcholine activated muscarinic receptors and evoked rises in the intracellular Ca2+ concentration. Amplitudes of currents through voltage-gated Ca2+ channels in AL neurons were much larger than those of other neurons. Ca2+ channel blocker against N-type channels, omega-conotoxin GVIA reduced the current by about 90%, suggesting that N-type Ca2+ channels are expressed at high density in AL neurons.
These results indicate that chick AL neurons expressed functional muscarinic acetylcholine receptors and N-type Ca2+ channels.

Research Products

• [Journal Article] Combination of serum phosphorylated neurofilament heavy subunit and hyperintensity of intramedullary T2W on magnetic resonance imaging provides better prognostic value of canine thoracolumbar intervertebral disc herniation (2015)
  • Author(s): T. Mashita, H. Kamishina, Y. Nakamoto, Y. Akagi, A. Nakanishi, Y. Harasaki, T. Ozawa, T. Uemura, Y. Kobatake, S. Shimamura, N. Kitamura, S. Maeda, Y. Uzuka, J. Yasuda
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 4 Pages: 433-438
  • DOI: 10.1292/jvms.14-0582
  • NAID: 130005067025
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurons in the rat supraoptic nucleus (2015)
  • Author(s): Taiki Moriya, Rina Shibasaki, Tomohiko Kayano, Nami Takebuchi, Momoko Ichimura, Naoki Kitamura, Atsushi Asano, Yoshinao Z. Hosaka, Oksana Forostyak, Alexei Verkhratsky, Govindan Dayanithi, Izumi Shibuya
  • Journal Title: Cell Calcium Volume: 57 Issue: 1 Pages: 25-37
  • DOI: 10.1016/j.ceca.2014.11.003
  • Peer Reviewed
• [Journal Article] Activation of muscarinic acetylcholine receptors elevates intracellular Ca2+ concentrations in accessory lobe neurons of the chick. (2015)
  • Author(s): 32.K. Takahashi K, Kitamura N, Suzuki Y, Yamanaka Y, Shinohara H, Shibuya I
  • Journal Title: J Comp Physiol A Neuroethol Sens Neural Behav Physiol Volume: 201 Issue: 4 Pages: 385-394
  • DOI: 10.1007/s00359-014-0971-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gymnopilins, a product of a hallucinogenic mushroom, inhibit the nicotinic acetylcholine receptor (2014)
  • Author(s): Tomohiko Kayano, Naoki Kitamura*, Shunsuke Miyazaki, Tsuyoshi Ichiyanagi, Norihiro Shimomura, Izumi Shibuya, Tadanori Aimi
  • Journal Title: Toxicon Volume: 81 Pages: 23-31
  • DOI: 10.1016/j.toxicon.2014.01.014
  • Peer Reviewed
• [Journal Article] Voltage-gated Ca2+ channels in accessory lobe neurons of the chick. (2014)
  • Author(s): Suzuki Y, Kitamura N, Yamanaka Y, Shibuya I
  • Journal Title: J Comp Physiol A Neuroethol Sens Neural Behav Physiol Volume: 200 Issue: 8 Pages: 739-748
  • DOI: 10.1007/s00359-014-0917-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Oral administration of royal jelly restores tear secretion capacity in rat blink-suppressed dry eye model by modulating lacrimal gland function. (2014)
  • Author(s): Imada T, Nakamura S, Kitamura N, Shibuya I, Tsubota K
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: e106338-e106338
  • DOI: 10.1371/journal.pone.0106338
  • Peer Reviewed
• [Journal Article] Analysis of G-porotein-activated inward rectifying K+ (GIRK) channel currents upon GABAb receptor activation in rat supraoptic neurons. (2014)
  • Author(s): Nobuya Harayama, Tomohiko Kayano, Taiki Moriya, Naoki Kitamura, Izumi Shibuya, Keiko Tanaka-Yamamoto, Y Uezono, Yoichi Ueta, T Sata.
  • Journal Title: Brain Research Volume: 1591 Pages: 1-13
  • DOI: 10.1016/j.brainres.2014.10.022
  • Peer Reviewed
• [Journal Article] Chronic NGF treatment induces somatic hyperexcitability in cultured dorsal root ganglion neurons of the rat. (2013)
  • Author(s): T. Kayano, N. Kitamura, T. Moriya, T. Kuwahara, Y. Komagiri, E. C. Toescu, I. Shibuya
  • Journal Title: Biomedical Research Volume: 34 Pages: 329-342
  • NAID: 130004470855
  • Peer Reviewed
• [Presentation] 体性感覚ニューロンのcapsaicin応答に対するnoradrenalineの抑制作用 (2015)
  • Author(s): 松下有美，坂本恵，北村直樹，澁谷泉
  • Organizer: 第67回日本生理学会中国四国地方会
  • Place of Presentation: 米子コンベンションセンター（米子市）
  • Year and Date: 2015-10-24
• [Presentation] ラット視索上核ニューロンに発現する中枢性浸透圧感知分子の解明 (2015)
  • Author(s): 中島純一，守屋大樹，柴崎梨奈，北村直樹，浅野淳，澁谷泉
  • Organizer: 第67回日本生理学会中国四国地方会
  • Place of Presentation: 米子コンベンションセンター（米子市）
  • Year and Date: 2015-10-24
• [Presentation] Stable cultivation of poisonous mushroom, Gymnopilus junonius. (2015)
  • Author(s): Ryuta Sato, Yoshio Sakamoto, Naoki Kitamrua, Tsuyoshi Ichiyanagi, Tadanori Aimi, Norihiro Shimomura
  • Organizer: The 8th Meeting of Asia for Mushroom Science
  • Place of Presentation: 米子コンベンションセンター（米子市）
  • Year and Date: 2015-10-20
  • Int'l Joint Research
• [Presentation] ラット背根神経節ニューロンのcapsaicin応答に対するnoradrenalineの抑制作用 (2015)
  • Author(s): 松下有美，坂本恵，北村直樹，澁谷泉
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（十和田市）
  • Year and Date: 2015-09-07
• [Presentation] ラット視索上核ニューロンに発現するTRPV1関連分子の探索及び機能解析 (2015)
  • Author(s): 中島純一，守屋大樹，柴崎梨奈，北村直樹，浅野淳，澁谷泉
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（十和田市）
  • Year and Date: 2015-09-07
• [Presentation] RT-PCR and Ca2+ imaging analyses of osmoreceptor molecules in the rat SON (2014)
  • Author(s): 3. Taiki Moriya, Rina Shibasaki, Tomohiko Kayano, Nami Takebuchi, Momoko Ichimura, Naoki Kitamura, Atsushi Asano, Yoshinao Z. Hosaka, Oksana Forostyak, Alexej Verkhratsky, Govindan Dayanithi, Izumi Shibuya
  • Organizer: Neuroscience 2014
  • Place of Presentation: コンベンションセンター・ワシントンDC・米国
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] ラット視索上核ニューロンに発現するTRPV1の機能解析および浸透圧受容器発現細胞の解析 (2014)
  • Author(s): 1. 守屋大樹，柴崎梨奈，萓野智彦，竹渕奈美，市村桃子，北村直樹，浅野淳，保坂善真，Govindan Dayanithi，澁谷泉
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学・札幌市
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 神経成長因子により誘発された感覚ニューロンの異常興奮性へのTRPV1の関与 (2014)
  • Author(s): 2. 萓野智彦，永見英利香，北村直樹，澁谷泉
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学・札幌市
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 鶏accessory lobeニューロンの電位依存性Ca2+チャネル電流 (2013)
  • Author(s): 鈴木夕貴，北村直樹，澁谷泉
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] ラット視索上核ニューロンの浸透圧感知機構へのTRPV1の機能的な関与について (2013)
  • Author(s): 柴崎梨奈，竹渕奈美，北村直樹，澁谷泉
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] 三朝温泉水のアトピー性皮膚炎症状改善効果:NC/Ngaマウスを用いた科学的検証 (2013)
  • Author(s): 辻野久美子，椎名克秀，岡崎良，岡本芳晴，北村直樹，杉山晶彦，田中あかね，松田浩珍，日笠喜朗
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学