| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Electrical activity and changes in the intracellular Ca2+ concentration were measured from spinal accessory lobe (AL) neurons of the chick.
AL neurons showed no significant response to the stimulation with the hypertonic solution that caused increase in the cell volume and stretch of the cell membrane. The stimulation with acetylcholine activated muscarinic receptors and evoked rises in the intracellular Ca2+ concentration. Amplitudes of currents through voltage-gated Ca2+ channels in AL neurons were much larger than those of other neurons. Ca2+ channel blocker against N-type channels, omega-conotoxin GVIA reduced the current by about 90%, suggesting that N-type Ca2+ channels are expressed at high density in AL neurons.
These results indicate that chick AL neurons expressed functional muscarinic acetylcholine receptors and N-type Ca2+ channels.
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