Analysis of osmoreceptor molecules in the supraoptic nucleus of the hypothalamus

• Project/Area Number: 25450464
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tottori University
• Principal Investigator: Shibuya Izumi 鳥取大学, 農学部, 教授 (50162649)
• Co-Investigator(Kenkyū-buntansha): KITAMURA Naoki 鳥取大学, 農学部, 准教授 (80301951) ; ASANO Atsushi 鹿児島大学, 共同獣医学部, 教授 (90312404)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 浸透圧受容 / バゾプレシン / 視索上核 / パッチクランプ / 細胞内Ca2+ / TRPV1 / 体液浸透圧 / 細胞内Ca2+濃度 / 浸透圧 / Capsaicin / GIRK / オキシトシン / RT-PCR

Outline of Final Research Achievements

The regulatory mechanism for the body fluid osmolality is known to be precise and enable to control the large volume of the body water at a level of a few percent accuracy. The osmoreceptor is the molecule to sense changes in the body osmolality and the central osmoreceptor has not been elucidated to date. The purpose of the present study was to study the molecular identity of the central osmoreceptor. Our results suggest that the central osmoreceptor may be a heterotetramer comprised of the transient receptor potential vanilloid 1 (TRPV1) and its related molecules.

Research Products

• [Journal Article] Activation of muscarinic acetylcholine receptors elevates intracellular Ca2+ concentrations in accessory lobe neurons of the chick. (2015)
  • Author(s): 32.K. Takahashi K, Kitamura N, Suzuki Y, Yamanaka Y, Shinohara H, Shibuya I
  • Journal Title: J Comp Physiol A Neuroethol Sens Neural Behav Physiol Volume: 201 Issue: 4 Pages: 385-394
  • DOI: 10.1007/s00359-014-0971-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gymnopilins, a product of a hallucinogenic mushroom, inhibit the nicotinic acetylcholine receptor. (2014)
  • Author(s): T. Kayano, N. Kitamura, S. Miyazaki, T. Ichiyanagi, N. Shimomura, I. Shibuya, T. Aimi.
  • Journal Title: Toxicon Volume: 81 Pages: 23-31
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Voltage-gated Ca2+ channels in accessory lobe neurons of the chick. (2014)
  • Author(s): Y. Suzuki, N. Kitamura, Y. Yamanaka, I. Shibuya.
  • Journal Title: Journal of Comparative Physiology A Volume: 200 Pages: 739-748
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Oral administration of royal jelly restores tear secretion capacity in rat blink-suppressed dry eye model by modulating lacrimal gland function. (2014)
  • Author(s): T. Imada, S. Nakamura, N. Kitamura, I. Shibuya, K. Tsubota.
  • Journal Title: PLoS ONE Volume: 9
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of G-protein-activated inward rectifying K+ (GIRK) channel currents upon GABAB receptor activation in rat supraoptic neurons. (2014)
  • Author(s): N. Harayama, T. Kayano, T. Moriya, N. Kitamura, I. Shibuya, K. Tanaka-Yamamoto, Y. Uezono, Y. Ueta, T. Sata.
  • Journal Title: Brain Research Volume: 1591 Pages: 1-13
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus. (2014)
  • Author(s): T. Moriya, R. Shibazaki, T. Kayano, N. Takebuchi, M. Ichimura, N. Kitamura, A. Asano, Y. Z. Hosaka, O. Forostyak, A. Verkhratsky, G. Dayanithi, I. Shibuya.
  • Journal Title: Cell Calcium Volume: 57 Pages: 25-37
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Chronic NGF treatment induces somatic hyperexcitability in cultured dorsal root ganglion neurons of the rat. (2013)
  • Author(s): T. Kayano, N. Kitamura, T. Moriya, T. Kuwahara, Y. Komagiri, E. C. Toescu, I. Shibuya.
  • Journal Title: Biomedical Research Volume: 34 Pages: 329-342
  • NAID: 130004470855
  • Peer Reviewed
• [Journal Article] Glutamate evokes firing through activation of kainate receptors in chick accessory lobe neurons. (2013)
  • Author(s): Y. Yamanaka, N. Kitamura, H. Shinohara, K. Takahashi, I. Shibuya.
  • Journal Title: Journal of Comparative Physiology A Volume: 199 Issue: 1 Pages: 35-43
  • DOI: 10.1007/s00359-012-0766-6
  • Peer Reviewed
• [Presentation] 体性感覚ニューロンのcapsaicin応答に対するnoradrenalineの抑制作用 (2015)
  • Author(s): 松下有美，坂本恵，北村直樹，澁谷泉．
  • Organizer: 第67回日本生理学会中国四国地方会
  • Place of Presentation: 米子
  • Year and Date: 2015-10-25
• [Presentation] ラット視索上核ニューロンに発現する中枢性浸透圧感知分子の解明 (2015)
  • Author(s): 中島純一，守屋大樹，柴崎梨奈，北村直樹，浅野淳，澁谷泉
  • Organizer: 第67回日本生理学会中国四国地方会
  • Place of Presentation: 米子
  • Year and Date: 2015-10-25
• [Presentation] ラット背根神経節ニューロンのcapsaicin応答に対するnoradrenalineの抑制作用 (2015)
  • Author(s): 松下有美，坂本恵，北村直樹，澁谷泉．
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 十和田
  • Year and Date: 2015-09-07
• [Presentation] ラット視索上核ニューロンに発現するTRPV1関連分子の探索及び機能解析 (2015)
  • Author(s): 中島純一，守屋大樹，柴崎梨奈，北村直樹，浅野淳，澁谷泉．
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 十和田
  • Year and Date: 2015-09-07
• [Presentation] RT-PCR and Ca2+ imaging analyses of osmoreceptor molecules in the rat SON. (2014)
  • Author(s): Taiki Moriya, Rina Shibasaki, Tomohiko Kayano, Nami Takebuchi, Momoko Ichimura, Naoki Kitamura, Atsushi Asano, Yoshinao Z. Hosaka, Oksana Forostyak, Alexej Verkhratsky, Govindan Dayanithi, Izumi Shibuya.
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] ラット視索上核ニューロンに発現するTRPV1の機能解析および浸透圧受容器発現細胞の解析 (2014)
  • Author(s): 守屋大樹，柴崎梨奈，萓野智彦，竹渕奈美，市村桃子，北村直樹，浅野淳，保坂善真，Govindan Dayanithi，澁谷泉．
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 神経成長因子により誘発された感覚ニューロンの異常興奮性へのTRPV1の関与 (2014)
  • Author(s): 萓野智彦，永見英利香，北村直樹，澁谷泉．
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 鶏accessory lobeニューロンの電位依存性Ca2+チャネル電流 (2013)
  • Author(s): 鈴木夕貴，北村直樹，澁谷泉
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）
• [Presentation] ラット視索上核ニューロンの浸透圧感知機構へのTRPV1の機能的な関与について (2013)
  • Author(s): 柴崎梨奈，竹渕奈美，北村直樹，澁谷泉
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）