Immunological study on bovine leukemia virus infection

• Project/Area Number: 25450468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: University of Miyazaki
• Principal Investigator: Norimine Junzo 宮崎大学, 産業動物リサーチセンター, 教授 (30627667)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 牛白血病 / 牛白血病ウイルス / 抵抗性遺伝子 / テトラマー / CD4T細胞 / MHC class II / ウイルス量 / 免疫防御 / 抵抗性 / DRB3

Outline of Final Research Achievements

Because approximately 70% of cattle infected with bovine leukemia virus remain healthy for their lives, it has been thought that there exist resistant genes exist against this disease. This study confirmed one of the resistant genes in both dairy and Japanese black cattle. The field study has demonstrated that cattle with the resistant gene could be utilized as a protectiveon wall for the infection. The investigator also developed the first immunological analytic tool, a bovine immunological “tetramer”, and is currently studying the resistant mechanisms using the tool.

Research Products

• [Journal Article] Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan (2015)
  • Author(s): Mekata H, Sekiguchi S, Konnai S, Kirino Y, Horii Y, Norimine J
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 9 Pages: 1115-1120
  • DOI: 10.1292/jvms.14-0624
  • NAID: 130005100718
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of the natural perinatal transmission of bovine leukaemia virus (2015)
  • Author(s): Mekata H, Sekiguchi S, Konnai S, Kirino Y, Honkawa K, Nonaka N, Horii Y, Norimine J.
  • Journal Title: Vet Rec Volume: 176(10) Issue: 10 Pages: 254-254
  • DOI: 10.1136/vr.102464
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Eradication of Bovine Leukaemia Virus infection at a regional level in Kyushu, Japan: Scenario tree modelling of the surveillance system. (2015)
  • Author(s): Yumi Kirino, Takashi Taniguchi, Sayaka Ueno, Hiroshi Kudoh, Hirohisa Mekata, Nariaki Nonaka, Yoichiro Horii, Junzo Norimine, Satoshi Sekiguchi
  • Organizer: 14th International Symposium for Veterinary Epidemiology and Economics
  • Place of Presentation: Merida Mexico
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] 牛白血病ウイルスの感染ウイルス量と予後の関係, (2015)
  • Author(s): 目堅博久, 関口敏, 今内覚, 堀井洋一郎, 乗峰潤三
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学 青森県
  • Year and Date: 2015-09-07
• [Presentation] 妊娠期及び分娩時の牛白血病ウイルス垂直感染リスクの解析 (2015)
  • Author(s): 目堅博久、関口敏、本川和幸、今内覚、堀井洋一郎、乗峰潤三
  • Organizer: 日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 岡山コンベンションセンター（岡山）
  • Year and Date: 2015-02-13 – 2015-02-15
• [Presentation] 若齢の白血病発症牛で認められた牛白血病ウイルスのenv領域188アミノ酸の脱落 (2014)
  • Author(s): 目堅博久、保田昌宏、張維東、関口敏、今内覚、乗峰潤三
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 地域レベルでの牛白血病ウイルス清浄化対策から明らかになったこと (2014)
  • Author(s): 目堅博久、関口敏、桐野有美、今内覚、堀井洋一郎、乗峰潤三
  • Organizer: 日本獣医師会学会
  • Place of Presentation: 千葉
• [Presentation] 牛白血病ウイルス感染に関する定性的リスク評価モデルの確立とその実用性の検討 (2014)
  • Author(s): 高木信乃、目堅博久、桐野有美、堀井洋一郎、乗峰潤三、関口敏
  • Organizer: 日本獣医師会学会
  • Place of Presentation: 千葉