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The role of IA-2 family proteins in the endocrine cells of gastrointestinal tract

Research Project

Project/Area Number 25450471
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionNihon University

Principal Investigator

GOMI Hiroshi  日本大学, 生物資源科学部, 教授 (90293240)

Co-Investigator(Renkei-kenkyūsha) TORII Seiji  群馬大学, 生体調節研究所, 准教授 (40312904)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsIA-2ファミリータンパク質 / 消化管内分泌細胞 / フォグリン / 分泌顆粒 / ペプチドホルモン / セクレトグラニン / 膵β細胞 / 膵島 / IA-2ファミリー蛋白質
Outline of Final Research Achievements

IA-2 family proteins (phogrin and IA-2) were identified by immunohistochemistry in the digestive tract of rodents. The difference in expression profile between phogrin and IA-2 was seems to be associated with cell maturity or proliferation. The possibility of phogrin as a gastrointestinal endocrine cell marker was suggested from the expression analysis of the intestinal epithelial cells after recovering from ex vivo enzyme digestion. Involvement of phogrin in the proteolysis of insulin granules and no significant change in cell proliferation at steady-state without continuous secretory stimulus were shown by analyzing the tissue of the pancreatic β-cell-specific phogrin KO mouse provided from cooperation researcher, Dr. S. Torii (Gunma Univ.).
A systemic expression profile of secretogranin III (Sg3), one of the phogrin-binding molecule, was analyzed in chicken. Sg3 was specifically expressed in cells that contain mature peptide hormones by prohormone-processing and bioactive amines.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.2015

    • Author(s)
      Gomi H, Morikawa S, Shinmura N, Moki H, Yasui T, Tsukise A, Torii S, Watanabe T, Maeda Y, Hosaka M
    • Journal Title

      J Histochem Cytochem

      Volume: 印刷中 Issue: 5 Pages: 350-366

    • DOI

      10.1369/0022155415575032

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Immunohistochemical analysis of IA-2 family of protein tyrosine phosphatases in rat gastrointestinal endocrine cells.2013

    • Author(s)
      Gomi H
    • Journal Title

      J Histochem Cytochem

      Volume: 61 Issue: 2 Pages: 156-168

    • DOI

      10.1369/0022155412466872

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ラット腸管内分泌細胞におけるIA2チロシンホスファターゼ分子群の発現様式(Immunohistochemical analysis of IA2 family of protein-tyrosine phosphatases in rat gastrointestinal endocrine cells)

    • Author(s)
      Hiroshi Gomi, Chisato Kubota-Murata, Seiji Torii
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Remarks] 日本大学生物資源科学部獣医学科研究室ホームページ

    • URL

      http://hp.brs.nihon-u.ac.jp/~vethome/laboratory/index.html

    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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