Studies on the mechanism of persistent infection of Bombyx mori macula-like virus in silkworm-derived cell lines.
Project/Area Number |
25450482
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
|
Research Institution | Utsunomiya University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | BmMLV / マキュラウイルス / カイコ / 持続感染 / 培養細胞 / チモウイルス |
Outline of Final Research Achievements |
The Bombyx mori macula-like virus (BmMLV) is a member of the genus Maculavirus, family Tymoviridae, and contains a positive-sense single-stranded RNA genome. Previously, we reported that almost all B. mori-derived cell lines have already been contaminated with BmMLV via an unknown infection route. To understand the mechanism of BmMLV propagation, we identified a novel subgenomic RNA of BmMLV. Also, we determined several viral inactivation methods by using the physical conditions. Furthermore, we revealed that BmMLV did not replicate in nine mammalian-derived cell lines. RNA-seq analysis was also performed to clarify the mechanism of BmMLV persistent infection.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Infection studies of nontarget mammalian cell lines with Bombyx mori macula-like virus.2016
Author(s)
Innami, K., Aizawa, T., Tsukui, T., Katsuma, S., Imanishi, S., Kawasaki, H., Iwanaga, M.
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Journal Title
J. Virol. Methods
Volume: 229
Pages: 24-26
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Inactivation of Bombyx mori macula-like virus under physical conditions.2016
Author(s)
Uchiyama, K., Fujimoto, H., Katsuma, S., Imanishi, S., Kato, A., Kawasaki, H., Iwanaga, M.
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Journal Title
In Vitro Cell. Dev. Biol. Anim.
Volume: 52
Issue: 3
Pages: 265-270
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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