| Project/Area Number |
25450489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MASE Keisuke 日本大学, 文理学部, 教授 (60414942)
YAMAHAMA Yumi 浜松医科大学, 医学部, 教務員 (90242784)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Bombyx mori / diapause / HCl / DMSO / カイコガ / 休眠 / 浸酸処理 / カルシウム / 休眠移行阻害 / DMSO処理 / 塩酸処理 / カイコ / 休眠卵 |
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| Outline of Final Research Achievements |
To elucidate the mechanism for preventing entry into embryonic diapause of the diapause of Bombyx mori by HCl, the quantitative analysis of Ca2+ and Mg2+ in the chorion and egg content was performed using ICP-AES. When diapause eggs were treated with HCl solution, the amount of Ca2+ in the chorion and egg content after HCl treatment was reduced to one-seventh, as compared with the amount before treatment. In contrast, there was no change in the amount of Mg2+ with HCl treatment. The amount of Ca2+ in the HCl solution after the diapause eggs were treated increased 7.5-fold, as compared with that of eggs treated with water. The quantitative analysis was also revealed that the amount of Ca2+ in diapause eggs was more than 2.4-fold larger than in non-diapause eggs.
Meanwhile, a gene of the mitochondrial Ca2+-dependent solute carrier protein (MCaSC) was also examined with HCl-treated diapause eggs. As a result, expression of MCaSC gene was induced by HCl treatment.
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