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Molecular basis of host regulation by symbiotic Spiroplasma

Research Project

Project/Area Number 25450492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

Kageyama Daisuke  国立研究開発法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 主任研究員 (60401212)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsスピロプラズマ / 共生細菌 / 昆虫 / 培養 / 垂直伝播
Outline of Final Research Achievements

During the process of searching for suitable Spiroplasma strains for research from nature, I found a Spiroplasma strain from a predatory flower bug of the genus Orius. This Spiropalsma strains was found cultivable in liquid as well as solid artificial media. I found some interesting features of proliferation that differ according to the different culture conditions-i.e., within insects, in the liquid medium, and on the solid medium, which requires further research. Attempt to extract plasmid genome following mass culture failed, probably indicating that this Spiroplasma strain lacks plasmid. On the other hand, a male-killing Spiroplasma strain derived from a lacewing of the genus Mallada was not cultivable under the above conditions, reflecting the differences in the nutritional requirements and/or degree of intimacy with insects.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Open Access: 1 results,  Peer Reviewed: 5 results) Presentation (12 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] キタキチョウと共生細菌ボルバキア2016

    • Author(s)
      陰山大輔
    • Journal Title

      昆虫と自然

      Volume: 51(1) Pages: 5-8

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Double trouble: combined action of meiotic drive and Wolbachia feminisation in Eurema butterflies2015

    • Author(s)
      Kern P, Cook JM, Kageyama D, Riegler M
    • Journal Title

      Biology Letters

      Volume: 11 Issue: 5 Pages: 20150095-20150095

    • DOI

      10.1098/rsbl.2015.0095

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 昆虫の生殖を操作する共生細菌Wolbachiaの機能と特徴2014

    • Author(s)
      陰山大輔
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 243-249

    • Related Report
      2014 Research-status Report
    • Open Access
  • [Journal Article] ワラジムシ目の体内に生息する共生微生物の多様性と機能2014

    • Author(s)
      陰山大輔
    • Journal Title

      日本土壌動物学会誌Edaphologia

      Volume: 95 Pages: 7-14

    • NAID

      110009872648

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel strain of Spiroplasma found in flower bugs of the genus Orius (Hemiptera: Anthocoridae): transovarial transmission, coexistence with Wolbachia and varied population density2014

    • Author(s)
      Watanabe M, Yukuhiro F, Maeda T, Miura K, Kageyama D
    • Journal Title

      Microbial Ecology

      Volume: 67 Issue: 1 Pages: 219-228

    • DOI

      10.1007/s00248-013-0335-8

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Natural variation in population densities and vertical transmission rates of a Spiroplasma endosymbiont in Drosophila hydei.2013

    • Author(s)
      Osaka R, Ichizono T, Kageyama D, Nomura M, Watada M
    • Journal Title

      Symbiosis

      Volume: 60 Issue: 2 Pages: 73-78

    • DOI

      10.1007/s13199-013-0242-2

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Detection of Spiroplasma from the mite Macrocheles sp. (Acari; Macrochelidae) ectoparasitic to the fly Drosophila hydei (Diptera; Drosophilidae): a possible route of horizontal transmission?2013

    • Author(s)
      Osaka R, Watada M, Kageyama D, Nomura M
    • Journal Title

      Symbiosis

      Volume: 60 Issue: 2 Pages: 79-84

    • DOI

      10.1007/s13199-013-0241-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 細胞内共生細菌Wolbachiaの生殖操作の違いによる宿主ミトコンドリアの分化への影響キタキチョウにおける2系統のWolbachia(wCIとwFem)について2016

    • Author(s)
      宮田真衣, 陰山大輔, 野村昌史
    • Organizer
      日本昆虫学会第76回大会・第59回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 性決定システムの多様性:何が急速な進化をもたらしたか?2016

    • Author(s)
      陰山大輔
    • Organizer
      日本昆虫学会第76回大会・第59回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] オカダンゴムシ由来のイリドウイルスはコシビロダンゴムシに感染するか?2016

    • Author(s)
      加藤朱莉, 陰山大輔
    • Organizer
      日本昆虫学会第76回大会・第59回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 共生微生物によって操られる昆虫の性2015

    • Author(s)
      陰山大輔
    • Organizer
      2015年ショウジョウバエ多様性研究会
    • Place of Presentation
      国立遺伝研
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] オス殺し、メス化、マイオティックドライブ:共生細菌ボルバキアによる宿主生殖操作メカニズム対する進化的考察2015

    • Author(s)
      陰山大輔
    • Organizer
      日本昆虫学会第75回大会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダンゴムシを青くするイリドウイルスを共生細菌ボルバキアは抑えることができるか?2015

    • Author(s)
      加藤朱莉, 陰山大輔
    • Organizer
      日本昆虫学会第75回大会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 共生細菌による昆虫の生殖操作:そのメカニズムと生態への影響2014

    • Author(s)
      陰山大輔
    • Organizer
      第162回日本昆虫学会・第99回日本応用動物昆虫学会合同東海支部会,公開シンポジウム「昆虫のゲノム・発生・生態に深くかかわる共生細菌たち」
    • Place of Presentation
      静岡大学
    • Year and Date
      2014-12-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 種子島のキタキチョウにおけるメスに偏った性比異常:ボルバキアは宿主を絶滅させるか?2014

    • Author(s)
      陰山大輔
    • Organizer
      日本昆虫学会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Mechanism of Wolbachia-induced feminizing effect in Lepidoptera2014

    • Author(s)
      Kageyama D
    • Organizer
      8th International Wolbachia Conference
    • Place of Presentation
      Igls, Innsbruck, Austria
    • Year and Date
      2014-06-06 – 2014-06-11
    • Related Report
      2014 Research-status Report
  • [Presentation] A new molecular sexing technique for butterflies – does Wolbachia really feminise genetic males in Eurema?2014

    • Author(s)
      Kern P, Kageyama D, Cook JM, Riegler M
    • Organizer
      8th International Wolbachia Conference
    • Place of Presentation
      Igls, Innsbruck, Austria
    • Year and Date
      2014-06-06 – 2014-06-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 共生細菌ボルバキアによるキタキチョウのメス化と野外における性比異常2014

    • Author(s)
      陰山大輔
    • Organizer
      昆虫DNA研究会第11回研究集会
    • Place of Presentation
      つくば
    • Year and Date
      2014-05-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 共生細菌ボルバキアが昆虫の生態、行動、進化に与える影響2014

    • Author(s)
      陰山大輔
    • Organizer
      日本生物地理学会
    • Place of Presentation
      立教大学
    • Year and Date
      2014-04-13
    • Related Report
      2014 Research-status Report
  • [Book] 消えるオス2015

    • Author(s)
      陰山大輔
    • Total Pages
      208
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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