| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Fission yeast Pot1 binds telomere DNA, and Rqh1 is required for resolution of recombination intermediate. We have shown that the double mutant between pot1 and the helicase-dead point mutant of the RecQ helicase Rqh1 (rqh1-hd) is sensitive to microtubule drug and accumulates recombination intermediates at telomeres. In this research, we found that Spindle assembly checkpoint (SAC)-dependent prometaphase arrest occurred frequently in pot1 rqh1-hd double mutants. Our results indicate that the accumulation of recombination intermediates induces SAC-dependent prometaphase arrest.
|
