Mechanism that telomere defect make cell sensitive to anti-microtubule drug and their application
| Project/Area Number |
25450517
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
Ueno Masaru 広島大学, 先端物質科学研究科, 准教授 (90293597)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | テロメア / スピンドルチェックポイント / 分裂酵母 / 微小管阻害剤 / 抗がん剤 / 組換え / rDNA / DNA複製 / DNA組換え |
|---|
| Outline of Final Research Achievements |
Fission yeast Pot1 binds telomere DNA, and Rqh1 is required for resolution of recombination intermediate. We have shown that the double mutant between pot1 and the helicase-dead point mutant of the RecQ helicase Rqh1 (rqh1-hd) is sensitive to microtubule drug and accumulates recombination intermediates at telomeres. In this research, we found that Spindle assembly checkpoint (SAC)-dependent prometaphase arrest occurred frequently in pot1 rqh1-hd double mutants. Our results indicate that the accumulation of recombination intermediates induces SAC-dependent prometaphase arrest.
|
Report
(4 results)
Research Products
(14 results)
-
[Journal Article] Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in S. pombe.2015
Author(s)
Habib, G., K., A., Masuda, K., Yukawa, M., Tsuchiya, E., and *Ueno, M
-
Journal Title
Biochem. Biophys. Res. Commun.
Volume: 464
Issue: 1
Pages: 140-146
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Fission yeast Exo1 and Rqh1-Dna2 redundantly contribute to resection of uncapped telomeres2015
Author(s)
Nanbu, T., Nguyen, L. C., Habib, G., K., A., Hirata, N., Ukimori, S., Tanaka, D., Masuda, K., Takahashi, K., Yukawa, M., Tsuchiya, E., and *Ueno, M
-
Journal Title
PLoS One
Volume: 10
Issue: 10
Pages: e0140456-e0140456
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
