Development of insulin-release nanocapsules based on novel response mechanism

• Project/Area Number: 25460031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Sato Katsuhiko 東北大学, 薬学研究科(研究院), 助教 (80400266)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 機能性薄膜 / ドラックデリバリーシステム / カプセル / インスリン / フェニルボロン酸 / 過酸化水素 / マクロカプセル / インスリンＤＤＳ / ナノカプセル / 交互累積膜 / マイクロカプセル / グルコース

Outline of Final Research Achievements

Glucose-sensitive multilayer thin films were prepared by a layer-by-layer (LbL) deposition of phenylboronic acid (PBA)-bearing polyelectrolytes and poly(vinyl alcohol) (PVA) on the surface of glucose oxidase (GOx)-modified quartz slide. PBA and PVA layers were connected with each other through boronate ester bonds while GOx was immobilized electrostatically interactions. The films were stable in wide pH range from 3.0 to 9.0 in the absence of glucose. In contrast, the films decomposed in the presence of glucose. The glucose-induced decomposition of the film can be rationalized based on the scission of the carbon―boron bond of PBA residues in PBA-polymer chains by hydrogen peroxide (H2O2) produced through the GOx-catalyzed oxidation reaction of glucose. The glucose-sensitive multilayer film may be useful for constructing insulin delivery systems.

Research Products

• [Journal Article] Glucose-induced decomposition of layer-by-layer films composed of phenylboronic acid-bearing poly(allylamine) and poly(vinyl alcohol) under physiological conditions (2015)
  • Author(s): Katsuhiko Sato, Mao Takahashi, Megumi Ito, Eiichi Abe, Jun-Ichi Anzai
  • Journal Title: Journal of Materials Chemistry B Volume: 3 Issue: 39 Pages: 7796-7802
  • DOI: 10.1039/c5tb01006c
  • Peer Reviewed
• [Journal Article] H2O2-Induced Decomposition of Layer-by-Layer Films Consisting of Phenylboronic Acid-Bearing Poly(allylamine) and Poly(vinyl alcohol) (2014)
  • Author(s): Katsuhiko Sato, Mao Takahashi, Megumi Ito, Eiichi Abe, Jun-ichi Anzai
  • Journal Title: Langmuir Volume: 30 Issue: 31 Pages: 9247-9250
  • DOI: 10.1021/la501750s
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loading and release of fluorescent dye from layer-by-layer film-coated magnetic particles in response to hydrogen peroxide (2014)
  • Author(s): Katsuhiko Sato, Eiichi Abe, Mao Takahashi, Jun-ichi Anzai
  • Journal Title: Journal of Colloid and Interface Science Volume: 432 Pages: 92-97
  • DOI: 10.1016/j.jcis.2014.06.039
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Release of Insulin from Calcium Carbonate Microspheres with and without Layer-by-Layer Thin Coatings (2014)
  • Author(s): Katsuhiko Sato, Masaru Seno, Jun-Ichi Anzai
  • Journal Title: Polymers Volume: 14 Issue: 8 Pages: 3100-3105
  • DOI: 10.3390/polym6082157
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multilayer films composed of phenylboronic acid-modified dendrimers sensitive to glucose under physiological conditions (2014)
  • Author(s): Ryota Watahiki, Katsuhiko Sato, Satoshi Niina, Keisuke Suwa, Yuya Egawa, Toshinobu Seki, Jun-ichi Anzai
  • Journal Title: Journal of Materials Chemistry B Volume: 2 Issue: 35 Pages: 5809-5817
  • DOI: 10.1039/c4tb00676c
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Glucose-induced decomposition of multilayer film under physiological conditions (2015)
  • Author(s): Katsuhiko Sato, Eiichi Abe, Jun-ichi Anzai
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-17
• [Presentation] 過酸化水素およびグルコース分解ナノ薄膜の開発 (2015)
  • Author(s): 佐藤勝彦、阿部瑛一、安斉順一
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 福岡
  • Year and Date: 2015-09-11
• [Presentation] 生理条件下でグルコースに応答する多層薄膜の調製 (2015)
  • Author(s): 佐藤勝彦, 高橋麻緒, 安斉順
  • Organizer: 薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-28
• [Presentation] 交互累積法による機能性ナノ薄膜およびミクロカプセルの開発 (2014)
  • Author(s): 佐藤勝彦
  • Organizer: 第36 回東北薬学セミナー
  • Place of Presentation: 仙台
  • Year and Date: 2014-12-05
  • Invited
• [Presentation] 過酸化水素に応答する交互累積膜の調製
  • Author(s): 佐藤勝彦、高橋麻緒、阿部瑛一、安斉順一
  • Organizer: 薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 交互累積膜からのH2O2による色素放出
  • Author(s): 佐藤勝彦、阿部瑛一、安斉順一
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 仙台