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Association dynamics of transemrbane helices detected by single-molecle FRET

Research Project

Project/Area Number 25460034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionKyoto University

Principal Investigator

Yano Yoshiaki  京都大学, 薬学研究科(研究院), 助教 (60402799)

Co-Investigator(Renkei-kenkyūsha) KATSUMI Matsuzaki  京都大学, 薬学研究科, 教授 (00201773)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords膜タンパク質フォールディング / モデル膜貫通へリックス / 一分子FRET / 膜貫通ヘリックス / 一分子計測 / モデル膜貫通ヘリックス / 一分子蛍光エネルギー移動
Outline of Final Research Achievements

Membrane proteins are important drug targets. However, driving forces that determine stability of proteins in membranes are poorly understood. In this project, association-dissociation dynamics of transmembrane helices were studied by single molecule FRET in liposomes to elucidate the rule of protein sequences and membrane lipids on helix association. A GXXXG motif was confirmed to drive parallel association of transmembrane helices (AALALAA)3. Moreover, two-transmembrane peptides were found to form four-transmembrane bundle. Membrane cholesterol significantly affected self-association property of the transmembrane helices. These results demonstrate the importance of protein sequence, lipid composition, and their combination on the stability of intramembrane helix-helix interactions.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer2015

    • Author(s)
      Yano Y, Kondo K, Kitani R, Yamamoto A, Matsuzaki K
    • Journal Title

      Biochemistry

      Volume: 54 Issue: 6 Pages: 1371-1379

    • DOI

      10.1021/bi501528e

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 二回膜貫通へリックス間相互作用の一分子蛍光測定に用いるモデルペプチドの合成2016

    • Author(s)
      渡邊由宇太、近藤小太郎、矢野義明、松崎勝巳
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ペプチドを用いた膜蛋白質の構造形成駆動力計測とイメージング解析2016

    • Author(s)
      矢野義明
    • Organizer
      蛋白質研究所セミナーmechanism of biology on the memebrane
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Single-molecule FRET detection of GXXXG-mediated transmembrane helical interactions2015

    • Author(s)
      Yoshiaki Yano, Kotaro Kondo, and Katsumi Matsuzaki
    • Organizer
      Biophysical society 59th annual meeting
    • Place of Presentation
      Baltimore, ML, USA
    • Year and Date
      2015-02-07 – 2015-02-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 一分子FRET測定法を用いた,膜貫通ヘリックス間相互作用へのGXXXGモチーフの寄与の解明2014

    • Author(s)
      近藤小太郎、矢野義明、松崎勝巳
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 一分子蛍光測定法を用いた,膜貫通ヘリックス間相互作用へのGXXXGモチーフの寄与の解析2014

    • Author(s)
      近藤小太郎、矢野義明、松崎勝巳
    • Organizer
      第12回次世代を担う若手のためのフィジカル・ファーマフォーラム
    • Place of Presentation
      箱根高原ホテル(神奈川県)
    • Year and Date
      2014-07-14 – 2014-07-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Single-molecule detection of association-dissociation dynamics of transmembrane helices induced by cholesterol2013

    • Author(s)
      Yoshiaki Yano, Kotaro Kondo, Ryota Kitani and Katsumim Matsuzaki
    • Organizer
      4th Asia-Pacific International Peptide Symposium
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2013 Research-status Report
  • [Remarks] 京都大学大学院 薬学研究科 薬品機能解析学分野 ホームページ

    • URL

      http://www.pharm.kyoto-u.ac.jp/yakkai/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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