| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Membrane proteins are important drug targets. However, driving forces that determine stability of proteins in membranes are poorly understood. In this project, association-dissociation dynamics of transmembrane helices were studied by single molecule FRET in liposomes to elucidate the rule of protein sequences and membrane lipids on helix association. A GXXXG motif was confirmed to drive parallel association of transmembrane helices (AALALAA)3. Moreover, two-transmembrane peptides were found to form four-transmembrane bundle. Membrane cholesterol significantly affected self-association property of the transmembrane helices. These results demonstrate the importance of protein sequence, lipid composition, and their combination on the stability of intramembrane helix-helix interactions.
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