In situ monitoring of drug action using electrochemical sensors
| Project/Area Number |
25460036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Yasuda Women's University (2014-2015)
Okayama University (2013) |
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Principal Investigator |
KATSU Takashi 安田女子大学, 薬学部, 教授 (40112156)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tsuyoshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (40370134)
KATO Hisato 就実大学, 薬学部, 助手 (70639228)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | センサー / アセチルコリン / 海馬 / 覚醒マウス / 光増感剤 / 光不活性化 / シクロデキストリン / ライソシン / 乳頭体上核 / クロリン系光増感剤 / 膜透過 / イオンセンサー / 酸素電極 / ポルフィリン / 形態変化 / シクロデキストリン誘導 |
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| Outline of Final Research Achievements |
We have developed a miniaturized acetylcholine sensor with high sensitivity and high selectivity and applied it to monitor changes in the concentration of acetylcholine in the hippocampus of awake mice in situ. Also, we have applied electrochemical sensors, such as a K+ sensor and an oxygen electrode, to analyze how porphyrin and chlorin dyes photoinactivate cell membranes. Furthermore, we have applied a K+ sensor to cell membrane permeability assay to clarify the action mechanism of antimicrobial cyclodextrin derivatives with low hemolytic activity and a new antibiotic, lysocin, that targets menaquinone in the bacterial membrane.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Lysocin E is a new antibiotic that targets menaquinone in the bacterial membrane.2015
Author(s)
Hamamoto H, Urai M, Ishii K, Yasukawa J, Paudel A, Murai M, Kaji T, Kuranaga T, Hamase K, Katsu T, Su J, Adachi T, Uchida R, Tomoda H, Yamada M, Souma M, Kurihara H, Inoue M, Sekimizu K.
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Journal Title
Nature Chemical Biology
Volume: 11(2)
Issue: 2
Pages: 127-133
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Acetylcholine regulates epilepsy in a model of hippocampal sclerosis2015
Author(s)
Fujii, S., Urakawa, K., Sada, N., Wakasa, A., Katsu, T., Inoue, T.
Organizer
Neuroscience 2015
Place of Presentation
Kobe Convention Center, Kobe, Hyogo
Year and Date
2015-07-29
Related Report
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