| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
In this research, we have carried out structural study of a DNA glycosylase which repairs damaged DNA coupled with DNA replication. DNA glycosylase was expressed and prepared with high purity for crystallization. Crystals of the DNA glycosylase and DNA complex were obtained after the crystallization screening and modification of the conditions. X-ray diffraction experiments were carried out on beam lines at SPring-8 and Photon factory, and the crystal structure was determined. In addition, we measured the glycosylase activity of the wild type and its mutants, and revealed the structural insight into the DNA repair mechanism of the DNA glycosylase.
|
