Structural analysis of asymmetrically branched glycans

• Project/Area Number: 25460054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yamaguchi Yoshiki 国立研究開発法人理化学研究所, 糖鎖構造生物学研究チーム, チームリーダー (90323451)
• Co-Investigator(Kenkyū-buntansha): 李 秀栄 国立研究開発法人理化学研究所, 生命システム研究センター, 上級研究員 (50390670)
• Co-Investigator(Renkei-kenkyūsha): HANASHIMA Shinya 理化学研究所, 糖鎖構造生物学研究チーム, 研究員 (50373353) ; NAGAE Masamichi 理化学研究所, 糖鎖構造生物学研究チーム, 研究員 (60619873) ; SUGITA Yuji 理化学研究所, 杉田理論分子科学研究室, 主任研究員 (80311190)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖鎖 / レクチン / ダイナミクス / NMR / 分枝構造 / 相互作用 / Ｘ線結晶構造解析 / 分子動力学 / 立体構造 / Dectin-1 / βグルカン / 気相電気泳動法 / 大量発現 / Calsepa / PHA-E / DCIR / ZG16p / PIM / N型糖鎖 / バイセクトGlcNAc / GnT-III / CLEC-2 / DCIR2

Outline of Final Research Achievements

The presence of branched structures is a characteristic of glycans, rarely found in other biomolecules such as proteins or nucleic acids. To understand the biological significance of arm-specific carbohydrate recognition by lectins, it is a prerequisite to evaluate interactions with the asymmetric branched glycans. In this study, we focused on the asymmetry of each glycan branch and analyzed in detail the structure, dynamics and interaction based on experimental (NMR, ion mobility spectrometry) and theoretical (molecular dynamics simulation) approaches. We revealed the structural basis of branch asymmetry and its role in lectin recognition.

Research Products

• [Int'l Joint Research] Max Planck Institute(Germany)
• [Int'l Joint Research] Max Planck Institute(Germany)
• [Int'l Joint Research] Imperial College London(英国)
• [Journal Article] 3D structural analysis of protein O-mannosyl kinase, POMK, a causative gene product of dystroglycanopathy (2017)
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• [Journal Article] Crystallographic analysis of murine p24γ2 Golgi dynamics domain (2017)
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• [Journal Article] The muscular dystrophy gene TMEM5encodes a ribitol β1-4 Xylosyltransferase required for the functional glycosylation of dystroglycan. (2016)
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  • Journal Title: J. Biol. Chem Volume: 291 Issue: 47 Pages: 24618-24627
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  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 3D Structure and Interaction of p24β and p24δ Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport. (2016)
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• [Journal Article] Enhancement of solubility and yield of a β-glucan receptor Dectin-1 C-type lectin-like domain in Escherichia coli with a solubility-enhancement tag. Protein (2016)
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  • Journal Title: Expr Purif. Volume: 123 Pages: 97-104
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• [Journal Article] Crystal structure of human dendritic cell inhibitory receptor (DCIR) C-type lectin domain reveals the binding mode with N-glycan (2016)
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  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Atomic visualization of a flipped-back conformation of bisected glycans bound to specific lectins (2016)
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  • Journal Title: Sci Rep. Volume: 6 (22973) Issue: 1 Pages: 1-11
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• [Journal Article] Identification of a post-translational modification with ribitol-phosphate and its defect in muscular dystrophy (2016)
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  • NAID: 120005728438
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sugar recognition and protein-protein interaction of mammalian lectins conferring diverse functions (2015)
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  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defining the interaction of human soluble lectin ZG16p and mycobacterial phosphatidylinositol mannosides (2015)
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  • Journal Title: ChemBioChem Volume: 16 Issue: 10 Pages: 1502-11
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  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm (2015)
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  • Journal Title: Journal of Biological Chemistry Volume: 9 Issue: 9 Pages: 5484-5501
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  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Self-Assembled, π-Stacked Complex as a Finely-Tunable Magnetic Aligner for Biomolecular NMR Applications (2015)
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• [Journal Article] An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer’s disease (2015)
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  • Journal Title: EMBO Mol. Med. Volume: 7(2) Issue: 2 Pages: 175-89
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  • NAID: 120006799362
  • Peer Reviewed / Open Access
• [Journal Article] In situ visualization of a glycoform of transferrin: Localization of α2,6-sialylated transferrin in the liver (2015)
  • Author(s): Yuka Matsumoto, Toshie Saito, Kyoka Hoshi, Hiromi Ito, Yoshinobu Kariya, Masamichi Nagae, Yoshiki Yamaguchi, Yoshiaki Hagiwara, Noriaki Kinoshita, Ikuo Wada, Kiyoshi Saito, Takashi Honda and Yasuhiro Hashimoto
  • Journal Title: J. Biochem. Volume: 157(4) Issue: 4 Pages: 211-216
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  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Backbone 1H, 13C, and 15N resonance assignments of the Fc fragment of human immunoglobulin G glycoprotein (2015)
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  • Journal Title: Biomol. NMR Assign. Volume: - Issue: 2 Pages: 383-6
  • DOI: 10.1007/s12104-014-9586-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A platform of C-type lectin-like receptor CLEC-2 for binding O-glycosylated podoplanin and nonglycosylated rhodocytin (2015)
  • Author(s): Nagae M, Morita-Matsumoto K, Kato M, Kaneko MK, Kato Y, Yamaguchi Y.
  • Journal Title: Structure Volume: 22 Issue: 12 Pages: 1711-1722
  • DOI: 10.1016/j.str.2014.09.009
  • Peer Reviewed / Open Access
• [Journal Article] Different IVIG glycoforms affect in vitro inhibition of anti-ganglioside antibody-mediated complement deposition (2014)
  • Author(s): Sudo, M., Yamaguchi, Y, Spath, P.J., Matsumoto-Morita, K., Ong, B.K., Shahrizaila, N., and Yuki, N.
  • Journal Title: PLoS ONE Volume: 9(9) Issue: 9 Pages: e107772-e107772
  • DOI: 10.1371/journal.pone.0107772
  • Peer Reviewed / Open Access
• [Journal Article] Site-specific classification of N-linked oligosaccharides of the extracellular regions of Fcγ receptor IIIb expressed in baby hamster kidney cells (2014)
  • Author(s): N.Kawasaki, T.Okumoto, Y.Yamaguchi, N.Takahashi, W.H.Fridman, C.Sautes-Fridman, H.Yagi, and K.Kato
  • Journal Title: J. Glycomics Lipidomics Volume: 4 Issue: 02 Pages: 116-116
  • DOI: 10.4172/2153-0637.1000116
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synthesis of N-glycan units for assessment of substrate structural requirements of N-acetylglucosaminyltransferase III (2014)
  • Author(s): 2.Shinya Hanashima, Hiroaki Korekane, Naoyuki Taniguchi, Yoshiki Yamaguchi
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 24 Issue: 18 Pages: 4533-4537
  • DOI: 10.1016/j.bmcl.2014.07.074
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interaction of platelet endothelial cell adhesion molecule (PECAM) with α2,6-sialylated glycan regulates its cell surface residency and anti-apoptotic role (2014)
  • Author(s): Kitazume, S., Imamaki, R., Kurimoto, A., Ogawa, K., Kato, M., Yamaguchi, Y., Tanaka, K., Ishida, H., Ando, H., Kiso, M., Hashii, N., Kawasaki, N. and Taniguchi, N.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 40 Pages: 27604-27613
  • DOI: 10.1074/jbc.m114.563585
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Osteopontin O-glycosylation contributes to its phosphorylation and cell-adhesion properties (2014)
  • Author(s): Yoshinobu Kariya, Mayumi Kanno, Kana Matsumoto-Morita, Midori Konno, Yoshiki Yamaguchi and Yasuhiro Hashimoto
  • Journal Title: Biochem. J. Volume: 463(1) Issue: 1 Pages: 93-102
  • DOI: 10.1042/bj20140060
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The α-helical region in p24γ2 subunit of p24 protein cargo receptor is pivotal for the recognition and transport of glycosylphosphatidylinositol-anchored proteins. (2014)
  • Author(s): Theiler R, Fujita M, Nagae M, Yamaguchi Y, Maeda Y, Kinoshita T.
  • Journal Title: J Biol Chem. Volume: 289 Issue: 24 Pages: 16835-16843
  • DOI: 10.1074/jbc.m114.568311
  • Peer Reviewed / Open Access
• [Journal Article] Structural Basis for Multiple Sugar Recognition of Jacalin-Related Human ZG16p Lectin (2014)
  • Author(s): Kanagawa, M., Liu, Y., Hanashima, S., Ikeda, A., Chai, W., Nakano, Y., Kojima-Aikawa, K., Feizi, T., and Yamaguchi, Y.
  • Journal Title: J Biol Chem. Volume: 289(24) Issue: 24 Pages: 16954-65
  • DOI: 10.1074/jbc.m113.539114
  • Peer Reviewed / Open Access
• [Journal Article] Structural change of N-glycan exposes hydrophobic surface of human transferrin (2014)
  • Author(s): Masamichi Nagae, Kana Morita-Matsumoto, Seisuke Arai, Ikuo Wada, Yuka Matsumoto, Kiyoshi Saito, Yasuhiro Hashimoto and Yoshiki Yamaguchi
  • Journal Title: Glycobiology Volume: 24(8) Issue: 8 Pages: 693-702
  • DOI: 10.1093/glycob/cwu033
  • Peer Reviewed
• [Journal Article] Binding assay between murine Dectin-1 and β-glucan/DNA complex with quartz-crystal microbalance. (2014)
  • Author(s): Mochizuki S., Morishita H., Adachi Y., Yamaguchi Y., and Sakurai K.
  • Journal Title: Carbohydr Res. Volume: 391 Pages: 1-8
  • DOI: 10.1016/j.carres.2014.03.019
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional structural aspects of protein-polysaccharide interactions. (2014)
  • Author(s): Nagae M, Yamaguchi Y.
  • Journal Title: Int J Mol Sci. Volume: 15(3) Issue: 3 Pages: 3768-83
  • DOI: 10.3390/ijms15033768
  • Peer Reviewed
• [Journal Article] Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold (2014)
  • Author(s): M. Nagae, K. Soga, K. Morita-Matsumoto, S. Hanashima, A. Ikeda, K. Yamamoto, and Y. Yamaguchi
  • Journal Title: Glycobiology Volume: 24 Issue: 4 Pages: 368-78
  • DOI: 10.1093/glycob/cwu004
  • Peer Reviewed
• [Journal Article] NMR study of short β(1-3)-glucans provides insights into the structure and interaction with Dectin-1. (2014)
  • Author(s): Hanashima S, Ikeda A, Tanaka H, Adachi Y, Ohno N, Takahashi T, Yamaguchi Y.
  • Journal Title: Glycoconjugate Journal Volume: 31 Issue: 3 Pages: 199-207
  • DOI: 10.1007/s10719-013-9510-x
  • Peer Reviewed
• [Journal Article] Recognition of Bisecting N-Acetylglucosamine: Structural basis for asymmetric interaction with the mouse lectin dendritic cell inhibitory receptor 2 (2013)
  • Author(s): Masamichi Nagae
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 47 Pages: 33598-33610
  • DOI: 10.1074/jbc.m113.513572
  • Peer Reviewed
• [Journal Article] Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid : insight into the binding preference for polysialic acid. (2013)
  • Author(s): Nagae M, Ikeda A, Halle M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 47 Pages: 33784-33796
  • DOI: 10.1074/jbc.m113.496224
  • Peer Reviewed
• [Journal Article] Expression and structural characterization of anti-T-antigen single chain antibodies (scFvs) and analysis of their binding to T-antigen by surface plasmon resonance and NMR spectroscopy (2013)
  • Author(s): N. Yuasa, T. Koyama, G.P. Subedi, Y. Yamaguchi, M. Matsushita, and Y. Fujita-Yamaguchi
  • Journal Title: The Journal of Biochemistry Volume: 154 Issue: 6 Pages: 521-9
  • DOI: 10.1093/jb/mvt089
  • Peer Reviewed
• [Journal Article] NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. (2013)
  • Author(s): Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y.
  • Journal Title: Bioorg. Med. Chem. Volume: 21 Issue: 19 Pages: 6069-76
  • DOI: 10.1016/j.bmc.2013.07.023
  • Peer Reviewed
• [Journal Article] Structural analysis of N-glycans attached to pig kidney Na(+)/K(+)-ATPase (2013)
  • Author(s): M. Kanagawa, K. Matsumoto, N. Iwasaki, Y. Hayashi, and Y. Yamaguchi
  • Journal Title: Journal of Glycomics and Lipidomics Volume: S5 Issue: S5
  • DOI: 10.4172/2153-0637.s5-005
  • Peer Reviewed
• [Presentation] Mammalian C-type lectin receptors: ligand recognition and signaling (2017)
  • Author(s): Yamaguchi Y
  • Organizer: ACADEMIA Sinica
  • Place of Presentation: 理化学研究所（埼玉県和光市）
  • Year and Date: 2017-03-30
  • Int'l Joint Research
• [Presentation] ありふれた糖鎖がシグナルになる秘密： CLEC-2の立体構造から学ぶ (2017)
  • Author(s): 山口 芳樹
  • Organizer: 第3回レクチン利用技術研究会
  • Place of Presentation: 秋葉原コンベンションホール（東京都千代田区）
  • Year and Date: 2017-03-11
  • Invited
• [Presentation] 予想もできなかった糖鎖認識の巧妙さ：バイセクト糖鎖結合レクチンを中心に (2016)
  • Author(s): 山口 芳樹
  • Organizer: 第2回レクチン利用技術研究会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2016-12-05
  • Invited
• [Presentation] 生体内糖鎖認識の基盤と次世代セラノスティクス (2016)
  • Author(s): 山口芳樹、田中克典
  • Organizer: 第14回糖質学会コンソーシアムシンポジウム（JCGG)
  • Place of Presentation: ソラシティコンファレンスセンター（東京都千代田区）
  • Year and Date: 2016-11-01
• [Presentation] Glycan dynamics analysed by combined NMR and X-ray crystallography (2016)
  • Author(s): Yamaguchi Y, Nagae M, Hanashima S
  • Organizer: 8th ACGG Annual Conference
  • Place of Presentation: Wuxi, China
  • Year and Date: 2016-10-15
  • Int'l Joint Research
• [Presentation] レクチンを利用したN-結合型糖鎖の準安定構造の解析 (2016)
  • Author(s): 山口 芳樹、長江 雅倫、花島 慎弥
  • Organizer: 第17回若手NMR研究会in箱根
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄下郡）
  • Year and Date: 2016-09-11
• [Presentation] 分岐型糖鎖の非対称性とダイナミクス (2016)
  • Author(s): 山口 芳樹
  • Organizer: 第35回 日本糖質学会年会 ワークショップ
  • Place of Presentation: 高知市文化プラザかるぽーと（高知県高知市）
  • Year and Date: 2016-09-03
• [Presentation] An Approach to Detect a Metastable Minor Conformation of Branched N-glycans by Combined Analysis of Solution NMR and X-ray Crystallography (2016)
  • Author(s): Yamaguchi Y
  • Organizer: ICMRBS2016 International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-08-25
  • Int'l Joint Research
• [Presentation] Understanding glycan dynamics and interaction by NMR and X-ray crystallography (2016)
  • Author(s): Yamaguchi Y
  • Organizer: Warren Workshop Ⅵ 2016
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-08-25
  • Int'l Joint Research / Invited
• [Presentation] Ion mobility spectrometry separation of isomeric N-glycan structures (2016)
  • Author(s): Yamaguchi Y, Hirose K, Nishima W, Re S, Sugita Y
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology ５th Annual Symposium
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2016-04-18
  • Int'l Joint Research
• [Presentation] Atomic level visualization of flipped back conformation of bisected glycan, implication for the conformer selection mechanism by lectins (2016)
  • Author(s): Masamichi Nagae
  • Organizer: The 3rd International Symposium on Glyco-Neuroscience
  • Place of Presentation: 淡路夢舞台国際会議場 （兵庫県淡路市）
  • Year and Date: 2016-01-14
  • Int'l Joint Research
• [Presentation] Glycan conformation, dynamics and interaction with lectin receptors: a structural glycobiology approach (2015)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド （兵庫県神戸市）
  • Year and Date: 2015-12-01
  • Int'l Joint Research
• [Presentation] Special topics lecture 1 "antibody structure, glycosylation and function" (2015)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: City of Hope
  • Place of Presentation: Duarte, USA
  • Year and Date: 2015-11-16
  • Invited
• [Presentation] NMRと分子力場計算を併用した糖鎖の立体構造とダイナミクスに関する研究 (Study on 3D structure and dynamics of sialo-glycans using NMR and MD) (2015)
  • Author(s): 鵜澤 洵
  • Organizer: 第54回NMR討論会(2015)
  • Place of Presentation: 千葉工業大学 津田沼キャンパス （千葉県習志野市）
  • Year and Date: 2015-11-08
• [Presentation] Atomic-level Visualization of Flipped-back Conformation of Bisected N-glycans (2015)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: ACADEMIA Sinica
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-10-15
  • Int'l Joint Research / Invited
• [Presentation] 分岐型糖鎖の非対称性とダイナミクス (2015)
  • Author(s): 山口芳樹
  • Organizer: 第9回東北薬科大学附属分子生体膜研究所、第9回東北糖鎖研究会 合同シンポジウム
  • Place of Presentation: 東北薬科大学 70周年記念講堂 （宮城県仙台市）
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] ヒト髄液中に存在する糖タンパク質の構造解析 (2015)
  • Author(s): 山口芳樹
  • Organizer: ENGase研究会第1回シンポジウム
  • Place of Presentation: 理化学研究所 大河内ホール （埼玉県和光市）
  • Year and Date: 2015-07-30
• [Presentation] アミロイド繊維と低分子化合物の相互作用 (Molecular interactions between amyloid fibrils and low-molecular-weight compounds) (2015)
  • Author(s): 山口 芳樹
  • Organizer: 第15回日本蛋白質科学年会
  • Place of Presentation: あわぎんホール （徳島県徳島市）
  • Year and Date: 2015-06-26
• [Presentation] Glycan conformation, dynamics and interaction with lectin receptors: a structural biology approach (2015)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, 4th Annual Symposium
  • Place of Presentation: ニチイ学館神戸ポートアイランドセンター (兵庫県神戸市）
  • Year and Date: 2015-05-12
  • Int'l Joint Research
• [Presentation] The indirect effect of bisecting GlcNAc on binding to PHA-E lectin: A possible role for conformer selection (2014)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: 6th Asian Communmity of Glycoscience and Glycotechnology
  • Place of Presentation: University of Hyderabad （Hyderabad, India）
  • Year and Date: 2014-12-12
  • Invited
• [Presentation] 糖鎖のタンパク質による認識－構造生物学によるアプローチ (2014)
  • Author(s): 山口芳樹
  • Organizer: 第12回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京医科歯科大学 （東京都）
  • Year and Date: 2014-12-05
  • Invited
• [Presentation] 糖鎖の立体構造とダイナミクス：機能との関係 (2014)
  • Author(s): 山口芳樹
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館 （京都府）
  • Year and Date: 2014-10-17
• [Presentation] 糖鎖の構造と相互作用：構造生物学によるアプローチ (2014)
  • Author(s): 山口芳樹
  • Organizer: 神戸大学医学部学術講演
  • Place of Presentation: 神戸大学 （兵庫県）
  • Year and Date: 2014-10-16
  • Invited
• [Presentation] 糖鎖構造生物学における未解明問題への取り組み (2014)
  • Author(s): 山口芳樹
  • Organizer: NIRFプロジェクト第7回研究会
  • Place of Presentation: 一橋大学 （東京都）
  • Year and Date: 2014-08-30
  • Invited
• [Presentation] イオンモビリティーとLC-MSの組み合わせによる迅速な糖鎖異性体の同定 (2014)
  • Author(s): 山口芳樹
  • Organizer: MSフォーラム
  • Place of Presentation: 日本ウォーターズ （東京都）
  • Year and Date: 2014-07-22
  • Invited
• [Presentation] The effect of bisecting GlcNAc is indirect on binding to PHA-E lectin: A possible role for conformer selection (2014)
  • Author(s): Masamichi Nagae, Keisuke Soga, Kana Morita-Matsumoto, Shinya Hanashima, Akemi Ikeda, Kazuo Yamamoto and Yoshiki Yamaguchi
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology 3rd Annual Symposium
  • Place of Presentation: Max Planck (Kreuth, Germany)
  • Year and Date: 2014-05-22
  • Invited
• [Presentation] H/D exchange：Applications in NMR and MS (2014)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: 第5回生体分子相互作用解析フォーラム ”HDX-MSワークショップ”
  • Place of Presentation: 日本ウォーターズ （大阪府）
  • Year and Date: 2014-04-22
  • Invited
• [Presentation] Defining the interaction of human soluble lectin ZG16p and bacterial phosphatidylinositol mannosides
  • Author(s): S. Hanashima, S. Götze, Y. Liu, M. Kato, A. Ikeda, K. Kojima-Aikawa, N. Taniguchi, D.V. Silva, T. Feizi, P. H. Seeberger and Y. Yamaguchi
  • Organizer: RIKEN-MAXPLANCK JOINT RESEARCH CENTER, 2nd Symposiuim
  • Place of Presentation: 理化学研究所 （埼玉県和光市）
• [Presentation] Confident Identification of Isomeric N-glycan Structures by Combined Ion Mobility Mass Spectrometry and Hydrophilic Interaction Liquid Chromatograph
  • Author(s): Y. Yamaguchi, K. Hirose, W. Nishima, S. Re and Y. Sugita
  • Organizer: American Society for Mass Spectrometry (ASMS)
  • Place of Presentation: Minneapolis, USA
• [Presentation] Experimental and theoretical approaches to understand the conformational differences in isomeric
  • Author(s): Y. Yamaguchi, K. Matsumoto, K. Hirose, W. Nishima, S. Re, Y. Sugita
  • Organizer: 22nd International Symposium on Glycoconjugates
  • Place of Presentation: Dalian, China
  • Invited
• [Presentation] Application of NMR to glycan interaction and dynamics
  • Author(s): Y. Yamaguchi
  • Organizer: The 3rd Austria / Japan Seminar on Comparative and Developmental Glycobiology
  • Place of Presentation: 理化学研究所 （埼玉県和光市）
• [Presentation] Temperature-dependent NOE analysis for the elucidation of glycan dynamics
  • Author(s): J. Uzawa, S. Hanashima, H. Seki, and Y. Yamaguchi
  • Organizer: The 3rd Austria / Japan Seminar on Comparative and Developmental Glycobiology
  • Place of Presentation: 理化学研究所 （埼玉県和光市）
• [Presentation] 糖鎖の立体構造とダイナミクス：機能との関係
  • Author(s): 山口芳樹
  • Organizer: Glyco TOKYO 2013 シンポジウム
  • Place of Presentation: 成蹊大学 （東京都武蔵野市）
  • Invited
• [Presentation] 合成基質を用いたシアロ糖鎖のNOEと縦緩和時間（T１）解析による ダイナミックス研究
  • Author(s): 鵜澤洵、阿部大典、関宏子、 桝飛雄真、山口芳樹
  • Organizer: Glyco TOKYO 2013 シンポジウム
  • Place of Presentation: 成蹊大学 （東京都武蔵野市）
• [Presentation] βグルカンの立体構造と機能：タンパク質との相互作用との関係
  • Author(s): 山口芳樹
  • Organizer: 第1回 β-グルカンフォーラム
  • Place of Presentation: 東京薬科大学 （東京都千代田区）
• [Presentation] NMRによるシアロ糖鎖の緩和機構とダイナ ミクスの研究
  • Author(s): 鵜澤洵、関宏子、桝飛雄真、 山口芳樹
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 石川県金沢市
• [Presentation] Glycan dynamics and interaction: From a 3D structural view
  • Author(s): 山口芳樹
  • Organizer: 理研-糖鎖インフォマティクス若手の会合同セミナー
  • Place of Presentation: 理化学研究所 （埼玉県和光市）
• [Presentation] 6位にグリコシド結合を持つ糖鎖のNMRによる配座解析
  • Author(s): 鵜澤洵、関宏子、桝飛雄真、 山口芳樹
  • Organizer: 日本農芸化学会2014年度
  • Place of Presentation: 明治大学 （神奈川県川崎市）
• [Book] NMR in Glycoscience and Glycotechnology, Chpter8 (2017)
  • Author(s): Yamaguchi, Y., Yagi, H., and Kato, K.
  • Total Pages: 458
  • Publisher: Royal Society of Chemistry
• [Book] Glycoscience: Biology and Medicine Indirect detection of hydroxy proton exchange through deuterium-induced 13C-NMR isotope shifts (2014)
  • Author(s): Hanashima, S.and Yamaguchi, Y.
  • Publisher: Springer
• [Book] Adv Neurobiol Structural Analysis of oligosaccharides and glycoconjugates using NMR (2014)
  • Author(s): Yamaguchi, Y., Yamaguchi, T., and Kato, K.
  • Total Pages: 566
  • Publisher: Springer
• [Remarks] 筋ジストロフィー症の原因となる糖鎖構造を解明
  • URL: http://www.tmghig.jp/J_TMIG/images/press/pdf/press20161017.pdf
• [Remarks] 報道発表資料 腸管免疫システムにおけるレクチンの新たな機能を発見
  • URL: http://www.riken.jp/pr/press/2015/20150521_1/
• [Remarks] 報道発表資料 折れ曲がった形の糖鎖を可視化
  • URL: http://www.riken.jp/pr/press/2016/20160323_2/
• [Remarks] 理化学研究所 研究紹介 糖鎖構造生物学研究チーム
  • URL: http://www.riken.jp/research/labs/grc/riken_max_planck/sys_glycobiol/struct_glycobiol/