Molecular mechanisms of presynaptic assembly in serotonergic neurons HSN in C. elegans

• Project/Area Number: 25460058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: TARU Hidenori 北海道大学, 薬学研究院, 准教授 (30533731)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経細胞 / シナプス / セロトニン作動性 / 神経科学

Outline of Final Research Achievements

At presynaptic structures in neurons, Cytomatrix at the Active Zone (CAZ)”, a group of unique adapter proteins, assembles and organizes functional molecules specialized for synaptic transmission. This project addressed the molecular mechanisms of presynaptic assembly mainly in serotonergic neurons HSN in nematode C. elegans: SYD-1, a key regulator of presynaptic assembly, negatively regulates GTPase through the binding of RhoGAP-like domain; RIMB-1, previously uncharacterized CAZ protein, regulates the localization of voltage-gated calcium channels at presynaptic structures.

Research Products

• [Journal Article] SYD-1C, UNC-40 (DCC) and SAX-3 (Robo) Function Interdependently to Promote Axon Guidance by Regulating the MIG-2 GTPase (2015)
  • Author(s): Yan Xu, Hidenori Taru, Yishi Jin, Christopher C. Quinn
  • Journal Title: PLoS Genetics Volume: 11 Issue: 4 Pages: e1005185-e1005185
  • DOI: 10.1371/journal.pgen.1005185
  • NAID: 120006318743
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cytoplasmic Fragment of Alcadein α Generated by Regulated Intramembrane Proteolysis Enhances Amyloid β-Protein Precursor (APP) Transport into the Late Secretory Pathway and Facilitates APP Cleavage (2015)
  • Author(s): Norio Takei, Yuriko Sobu, Ayano Kimura, Satomi Urano, Yi Piao, Yoichi Araki, Hidenori Taru, Tohru Yamamoto, Saori Hata, Tadashi Nakaya, Toshiharu Suzuki
  • Journal Title: Journal of Biological Chemistry Volume: 290 Issue: 2 Pages: 987-995
  • DOI: 10.1074/jbc.m114.599852
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport. (2014)
  • Author(s): Chiba K, Araseki M, Nozawa K, Furukori K, Araki Y, Matsushima T, Nakaya T,Hata S, Saito Y, Uchida S, Okada Y, Nairn AC, Davis RJ, Yamamoto T, Kinjo M, Taru, H, Suzuki T.
  • Journal Title: Mol Biol Cell Volume: 25 Issue: 22 Pages: 3569-3580
  • DOI: 10.1091/mbc.e14-06-1111
  • NAID: 120005522339
  • Peer Reviewed / Open Access
• [Journal Article] Increased levels of plasma p3-Alcα35, a major fragment of Alcadeinα by γ-secretase cleavage, in Alzheimer's disease (2014)
  • Author(s): Omori C, Kaneko M, Nakajima E, Akatsu H, Waragai M, Maeda M, Moroshima-Kawashima M, Saito Y, Nakaya T, Taru H, Yamamoto T, Asada T, Hata S, Suzuki T. for J-ADNI
  • Journal Title: J. Alzheimers Dis Volume: 39 Issue: 4 Pages: 861-870
  • DOI: 10.3233/jad-131610
  • Peer Reviewed
• [Journal Article] Liprin-alpha/SYD-2 determines the size of dense projections in presynaptic active zones in C. elegans (2013)
  • Author(s): Maike Kittelmann, Jan Hegermann, Alexandr Goncharov, Hidenori Taru, Mark H. Ellisman, Janet Richmond, Yishi Jin and Stefan Eimer
  • Journal Title: J.Cell Biology Volume: 203 Issue: 5 Pages: 849-863
  • DOI: 10.1083/jcb.201302022
  • NAID: 120005367408
  • Peer Reviewed
• [Journal Article] Mechanism of intramembrane cleavage of Alcadeins by γ-secretase (2013)
  • Author(s): Piao, Y., Kimura, A., Urano, S., Saito, Y., Taru, H., Yamamoto, T., Hata, S. and Suzuki, T
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e62431-e62431
  • DOI: 10.1371/journal.pone.0062431
  • Peer Reviewed
• [Presentation] Characterization of RIM-binding protein RIMB-1 in the presynaptic active zone (2016)
  • Author(s): Yuto Kushibiki, Toshiharu Suzuki, Hidenori Taru
  • Organizer: CeNeuro2016
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Genetic analysis of voltage-gated calcium channel localization and presynaptic structure by rimb-1 (2015)
  • Author(s): Yuto Kushibiki,Toshiharu Suzuki and Hidenori Taru
  • Organizer: 3rd International Life-Science Symposium
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-11-26
• [Presentation] Negative regulation of presynaptic assembly by a non-classical cadherin in serotonergic neurons in C. elegans (2014)
  • Author(s): Hidenori Taru and Toshiharu Suzuki
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: Milano Congressi
  • Year and Date: 2014-07-07
• [Presentation] Identification of a cell adhesion molecule involved in negative regulation of presynaptic assembly in C.elegans
  • Author(s): Hidenori Taru
  • Organizer: Experimental Biology 2013
  • Place of Presentation: ボストン国際会議場（USA)
• [Presentation] 神経シナプス形成機構への遺伝学的アプローチ
  • Author(s): 多留 偉功
  • Organizer: 日本生化学会北海道支部２０１３年度合同シンポジウム
  • Place of Presentation: 北海道大学（札幌）