Activation mechanism of unfolded protein response by membrane lipid saturation
Project/Area Number |
25460060
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | The University of Tokyo |
Principal Investigator |
Kono Nozomu 東京大学, 薬学研究科(研究院), 講師 (50451852)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | リン脂質 / 脂肪酸 / 小胞体ストレス応答 |
Outline of Final Research Achievements |
Increased levels of saturated fatty acids in membrane phospholipids (membrane lipid saturation) activated IRE1α and PERK, but did not activate ATF6α. A conventional ER stressor induced clustering of fluorescently tagged IRE1α fusion protein, but membrane lipid saturation did not. These results suggest membrane lipid saturation and unfolded proteins activate the UPR through different mechanisms. Analysis of an ER membrane protein involved in the UPR activation upon membrane lipid saturation revealed a homeostatic mechanism against membrane lipid saturation.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling. Exp. Cell Res. 342, 1-10.2016
Author(s)
Henmi, Y., Oe, N., Kono, N., Taguchi, T., Takei, K., and Tanabe, K.
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Journal Title
Exp. Cell Res.
Volume: 342
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Age-related changes of vitamin E: α-tocopherol levels in plasma and various tissues of mice and hepatic α-tocopherol transfer protein.2016
Author(s)
Takahashi, K., Takisawa, S., Shimokado, K., Kono, N., Arai, H., Ishigami, A.
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Journal Title
Eur J Nutr.
Volume: 印刷中
Issue: 3
Pages: 1317-1327
DOI
Related Report
Peer Reviewed
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[Journal Article] Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids.2016
Author(s)
Akagi, S., Kono, N., Ariyama, H., Shindou, H., Shimizu, T., and Arai, H.
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Journal Title
FASEB J.
Volume: 30
Issue: 5
Pages: 2027-2039
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] ABCB4 exports phosphatidylcholine in a sphingomyelin-dependent manner2015
Author(s)
Zhao, Y., Ishigami, M., Nagao, K., Hanada, K., Kono, N., Arai, H., Matsuo, M., Kioka, N., and Ueda, K.
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Journal Title
J. Lipid Res.
Volume: 56
Issue: 3
Pages: 644-652
DOI
Related Report
Peer Reviewed
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[Journal Article] Saturated fatty acid palmitate aggravates neointima formation by promoting smooth muscle phenotypic modulation.2013
Author(s)
Shen H, Eguchi K, Kono N, Fujiu K, Matsumoto S, Shibata M, Oishi-Tanaka Y, Komuro I, Arai H, Nagai R, Manabe I.
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Journal Title
Arterioscler Thromb Vasc Biol.
Volume: 33
Pages: 2596-2607
Related Report
Peer Reviewed
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[Journal Article] Mitochondria-type GPAT is required for mitochondrial fusion2013
Author(s)
Yohsuke Ohba, Takeshi Sakuragi, Eriko Kage-Nakadai, Naoko H Tomioka, Nozomu Kono, Rieko Imae, Asuka Inoue, Junken Aoki, Naotada Ishihara, Takao Inoue, Shohei Mitani and Hiroyuki Arai
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Journal Title
The EMBO Journal
Volume: (in press)
Issue: 9
Pages: 1265-1279
DOI
Related Report
Peer Reviewed / Open Access
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